Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   D9C20_RS21385 Genome accession   NZ_CP032867
Coordinates   3988978..3989598 (+) Length   206 a.a.
NCBI ID   WP_014480105.1    Uniprot ID   A0A0D1KQW4
Organism   Bacillus subtilis subsp. subtilis strain N4-2     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3983978..3994598
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D9C20_RS21345 (D9C20_21345) ptsA 3985110..3985616 (-) 507 WP_029726788.1 PTS glucose transporter subunit IIA -
  D9C20_RS21350 (D9C20_21350) ypqA 3985754..3986173 (+) 420 WP_014480103.1 Hsp20/alpha crystallin family protein -
  D9C20_RS21355 (D9C20_21355) sppG 3986194..3986571 (-) 378 WP_004398696.1 YppG family protein -
  D9C20_RS21360 (D9C20_21360) yppF 3986758..3986946 (+) 189 WP_003230690.1 YppF family protein -
  D9C20_RS21365 (D9C20_21365) yppE 3986985..3987356 (-) 372 WP_003246080.1 YppE family protein -
  D9C20_RS21370 (D9C20_21370) yppD 3987402..3987647 (-) 246 WP_004398488.1 YppD family protein -
  D9C20_RS21375 (D9C20_21375) sspM 3987846..3987950 (+) 105 WP_003225606.1 acid-soluble spore protein SspM -
  D9C20_RS21380 (D9C20_21380) yppC 3987975..3988937 (-) 963 WP_033881326.1 DUF2515 domain-containing protein -
  D9C20_RS21385 (D9C20_21385) recU 3988978..3989598 (+) 621 WP_014480105.1 Holliday junction resolvase RecU Machinery gene
  D9C20_RS21390 (D9C20_21390) ponA 3989620..3992364 (+) 2745 WP_004398589.1 PBP1A family penicillin-binding protein -
  D9C20_RS21395 (D9C20_21395) ypoC 3992440..3992934 (-) 495 WP_009967579.1 YpoC family protein -
  D9C20_RS21400 (D9C20_21400) nth 3992931..3993590 (-) 660 WP_009967580.1 endonuclease III -
  D9C20_RS21405 (D9C20_21405) dnaD 3993609..3994307 (-) 699 WP_004398499.1 DNA replication protein DnaD -

Sequence

Protein


Download         Length: 206 a.a.        Molecular weight: 24019.18 Da        Isoelectric Point: 9.6240

>NTDB_id=319843 D9C20_RS21385 WP_014480105.1 3988978..3989598(+) (recU) [Bacillus subtilis subsp. subtilis strain N4-2]
MIRYPNGKTFQPKHSVSSQNSQKRAPSYSNRGMTLEDDLNETNKYYLTNQIAVIHKKPTPVQIVNVHYPKRSAAVIKEAY
FKQSSTTDYNGIYKGRYIDFEAKETKNKTSFPLQNFHDHQIEHMKQVKAQDGICFVIISAFDQVYFLEADKLFYFWDRKE
KNGRKSIRKDELEETAYPIFLGYAPRIDYISIIEQLYFSPSSGAKG

Nucleotide


Download         Length: 621 bp        

>NTDB_id=319843 D9C20_RS21385 WP_014480105.1 3988978..3989598(+) (recU) [Bacillus subtilis subsp. subtilis strain N4-2]
GTGATTCGGTATCCTAACGGAAAAACATTTCAGCCGAAACATTCGGTTTCATCCCAAAACAGTCAGAAAAGGGCCCCGTC
TTACAGTAATCGCGGAATGACCCTCGAAGATGACTTAAACGAAACGAATAAGTATTATCTGACAAACCAAATTGCCGTTA
TACACAAAAAGCCGACACCTGTTCAAATTGTAAATGTCCATTATCCAAAAAGAAGTGCCGCAGTGATTAAAGAAGCTTAC
TTTAAACAATCATCGACAACAGACTACAATGGGATTTACAAAGGGCGGTATATTGATTTTGAGGCGAAAGAAACGAAAAA
CAAGACCTCTTTCCCCCTGCAGAATTTTCATGACCATCAAATCGAGCATATGAAGCAGGTAAAGGCTCAAGACGGTATTT
GTTTTGTTATTATATCCGCTTTCGACCAGGTATATTTTTTGGAAGCCGATAAGCTGTTTTATTTCTGGGACAGAAAAGAA
AAAAACGGCAGAAAATCAATTCGAAAAGATGAGTTGGAAGAAACAGCTTATCCGATTTTTCTTGGATACGCACCCAGAAT
TGATTATATTAGTATTATTGAACAGCTTTATTTTTCGCCATCATCTGGTGCGAAAGGTTGA

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(31-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1KQW4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

99.515

100

0.995

Multiple sequence alignment