Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   D9C10_RS12085 Genome accession   NZ_CP032853
Coordinates   2178444..2180804 (-) Length   786 a.a.
NCBI ID   WP_069837662.1    Uniprot ID   -
Organism   Bacillus subtilis subsp. subtilis strain MH-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2173444..2185804
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D9C10_RS12065 (D9C10_12065) yrvJ 2173518..2175074 (+) 1557 WP_033881623.1 SH3 domain-containing protein -
  D9C10_RS12070 (D9C10_12070) dtd 2175101..2175541 (-) 441 WP_004398689.1 D-aminoacyl-tRNA deacylase -
  D9C10_RS12075 (D9C10_12075) relA 2175554..2177758 (-) 2205 WP_003229747.1 GTP diphosphokinase -
  D9C10_RS12080 (D9C10_12080) aptA 2177926..2178438 (-) 513 WP_003229745.1 adenine phosphoribosyltransferase -
  D9C10_RS12085 (D9C10_12085) recJ 2178444..2180804 (-) 2361 WP_069837662.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  D9C10_RS12090 (D9C10_12090) yrvD 2180871..2181194 (-) 324 WP_003229740.1 lipopolysaccharide assembly LapA domain-containing protein -
  D9C10_RS12095 (D9C10_12095) yrvC 2181270..2181767 (-) 498 WP_014480433.1 cation:proton antiporter regulatory subunit -
  D9C10_RS12100 (D9C10_12100) secDF 2181925..2184138 (-) 2214 WP_121548959.1 protein translocase subunit SecDF -
  D9C10_RS12105 (D9C10_12105) comN 2184177..2184473 (-) 297 WP_004398498.1 post-transcriptional regulator ComN -

Sequence

Protein


Download         Length: 786 a.a.        Molecular weight: 88019.57 Da        Isoelectric Point: 5.0116

>NTDB_id=319255 D9C10_RS12085 WP_069837662.1 2178444..2180804(-) (recJ) [Bacillus subtilis subsp. subtilis strain MH-1]
MLASKMRWEIQRPDQDKVKSLTEQLHITPLVASLLVKRGFDTAESARLFLHTKDADFYDPFEMKGMKEAADRIKQAISQQ
EKIMIYGDYDADGVTSTSVMLHTLQKLSAQVDFYIPDRFKEGYGPNEQAFRSIKERGFSLIITVDTGIAAVHEAKVAKEL
GLDVIITDHHEPGPELPNVHAIVHPKQPGCTYPFKELAGVGVAFKLAHALLDELPDELLDLAAIGTIADLVPLHDENRLI
ATLGLERLRRTNRLGLKELIKLSGGDIGEANEETVGFQLAPRLNAVGRIEQADPAVHLLMSEDSFEAEELAAEIDQLNKE
RQKMVSKMTDEAIEMVEQQGLDQTAIVVAKAGWNPGVVGIVASKLVDRFYRPAIVLGIDEEKGIAKGSARSIRGFNLFES
LSECRDILPHFGGHPMAAGMTLKAEDVPDLRSRLNEIADNTLTEEDFIPVQEVDLVCGVEDITVESIAEMNMLSPFGMLN
PKPHVLVENAVLEDVRKIGANKTHVKMTIRNESSQLDCVGFNKGELEEGIVPGSRISIVGEMSINEWNNRKKPQLMIKDA
AVSEWQLFDLRGKRTWEDTVSALPSAKRAIVSFKEDSTTLLQTEGLRREVHVISSEDQAKAFDLDGAYIVLLDPPPSLDM
LARLLEGKAPERIYFIFLNHEDHFLSTFPARDHFKWYYAFLLKRGAFDVEKHASELAKHKGWSVETINFMTKVFFDLGFV
KIENGVLSVVSGAKKRDLTDSQTYQAKQQLMELDQKLNYSSAEELKEWLNKLMKQDSEAYESTRRT

Nucleotide


Download         Length: 2361 bp        

>NTDB_id=319255 D9C10_RS12085 WP_069837662.1 2178444..2180804(-) (recJ) [Bacillus subtilis subsp. subtilis strain MH-1]
ATGTTAGCGTCAAAAATGCGATGGGAAATCCAGCGACCAGATCAGGATAAGGTCAAATCACTCACAGAACAATTGCATAT
AACACCTCTTGTTGCGTCCCTGCTTGTAAAAAGAGGCTTTGATACGGCAGAAAGCGCTAGGCTGTTTTTACATACAAAGG
ATGCCGATTTTTATGATCCTTTTGAAATGAAAGGCATGAAAGAAGCGGCAGATCGAATAAAACAGGCGATTTCACAACAA
GAAAAGATTATGATATATGGCGATTATGACGCCGATGGGGTTACAAGTACGTCAGTAATGCTTCACACGCTGCAAAAACT
GTCGGCTCAAGTTGATTTTTATATACCTGACCGTTTTAAAGAAGGATATGGACCTAATGAACAGGCGTTTCGTTCGATTA
AAGAACGGGGCTTCTCTTTGATCATTACGGTTGATACAGGAATTGCCGCTGTGCATGAAGCGAAGGTGGCAAAGGAACTC
GGACTGGATGTCATCATTACCGATCATCATGAACCGGGACCGGAGCTTCCGAATGTCCATGCGATCGTCCACCCGAAGCA
GCCGGGCTGCACTTACCCGTTTAAAGAGCTTGCAGGGGTTGGAGTCGCGTTTAAGCTTGCCCACGCTTTATTGGATGAAC
TTCCTGATGAACTGCTTGACCTGGCGGCTATCGGCACCATTGCCGATCTAGTCCCGCTGCATGACGAAAACAGATTAATT
GCAACACTAGGACTTGAAAGGCTTCGACGGACAAATCGGCTCGGCTTAAAAGAGCTGATTAAGCTGTCAGGCGGAGACAT
CGGAGAAGCGAACGAAGAAACTGTCGGATTTCAGCTCGCACCAAGGCTGAATGCCGTCGGAAGAATCGAACAGGCGGATC
CCGCTGTTCATTTGCTTATGTCAGAGGATTCGTTTGAAGCGGAAGAGCTTGCCGCAGAAATTGACCAGCTGAACAAAGAG
CGCCAAAAAATGGTCAGCAAAATGACGGATGAAGCGATTGAAATGGTTGAACAGCAAGGGCTCGATCAAACTGCAATTGT
CGTGGCCAAAGCCGGATGGAACCCGGGAGTCGTCGGGATTGTGGCTTCAAAGCTTGTGGACCGTTTCTACCGACCGGCCA
TTGTTCTTGGTATTGATGAAGAAAAGGGAATCGCTAAGGGTTCGGCCCGAAGCATCAGAGGATTTAATTTATTTGAGAGC
CTTTCTGAATGCAGAGATATTCTTCCTCATTTCGGCGGCCATCCAATGGCTGCGGGGATGACGCTGAAAGCCGAAGATGT
GCCGGATTTGCGAAGCAGGCTGAATGAAATAGCGGACAATACGCTTACGGAAGAGGATTTTATTCCCGTACAGGAAGTCG
ACCTTGTCTGCGGCGTCGAAGACATTACAGTTGAAAGCATCGCAGAAATGAACATGCTGTCTCCTTTTGGCATGCTGAAC
CCGAAACCGCACGTTCTCGTAGAAAACGCAGTACTGGAAGATGTCCGCAAGATAGGGGCAAATAAAACTCATGTCAAAAT
GACGATCAGAAACGAGTCGTCCCAGCTTGATTGCGTCGGTTTTAATAAAGGCGAGCTTGAAGAAGGCATCGTACCGGGTT
CAAGAATTTCCATTGTCGGTGAGATGTCAATCAACGAATGGAACAATCGAAAAAAGCCGCAGCTTATGATAAAGGATGCC
GCTGTTTCTGAATGGCAGCTGTTTGATTTAAGGGGAAAACGGACGTGGGAAGACACAGTTTCCGCCCTTCCATCTGCTAA
ACGGGCCATTGTCTCTTTCAAAGAAGACTCAACAACACTGCTTCAAACTGAAGGCCTTCGCCGAGAAGTGCACGTCATCA
GCTCAGAAGATCAAGCAAAGGCGTTTGACCTCGATGGTGCATATATTGTATTACTGGATCCGCCGCCTTCGCTGGACATG
CTCGCACGTTTGCTGGAAGGAAAGGCGCCCGAGCGGATATATTTTATTTTTCTCAATCATGAGGACCACTTTTTATCAAC
TTTTCCCGCAAGAGACCACTTTAAATGGTATTATGCGTTCTTGCTGAAAAGAGGCGCTTTTGACGTGGAAAAACACGCTT
CAGAGCTTGCAAAACATAAAGGCTGGTCCGTTGAAACAATCAATTTCATGACAAAGGTGTTTTTTGATCTCGGTTTTGTT
AAAATAGAGAATGGTGTGCTGTCTGTTGTGAGCGGAGCAAAGAAACGCGACTTAACAGACTCACAAACATATCAGGCGAA
ACAGCAGCTGATGGAACTGGATCAAAAGCTGAATTATTCTTCTGCTGAAGAGCTGAAGGAATGGCTGAATAAGCTGATGA
AACAAGATTCAGAGGCTTATGAAAGTACGAGGAGGACATAA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(315-437)

RecJ OB domain (RecJ_OB)

(453-559)

DHH family (DHH)

(82-226)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-769)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

98.982

100

0.99

Multiple sequence alignment