Detailed information NTG0317796

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: E8M05_RS02465
• Genome accession: NZ_CP039457
• Coordinates: 439294..441684 (+)
• Length: 796 a.a.
• NCBI ID: WP_069789207.1
• Uniprot ID: -
• Organism: Streptococcus pasteurianus strain WUSP067
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 434294..446684

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E8M05_RS02440 (E8M05_02435)	-	434878..435642 (+)	765	WP_003063521.1	(S)-acetoin forming diacetyl reductase	-
E8M05_RS02445 (E8M05_02440)	-	435721..436206 (-)	486	WP_117479654.1	S-ribosylhomocysteine lyase	-
E8M05_RS02450 (E8M05_02445)	-	436357..437964 (+)	1608	WP_048791287.1	ribonuclease Y	-
E8M05_RS02455 (E8M05_02450)	gmk	438218..438835 (+)	618	WP_003063524.1	guanylate kinase	-
E8M05_RS02460 (E8M05_02455)	rpoZ	438858..439172 (+)	315	WP_013851522.1	DNA-directed RNA polymerase subunit omega	-
E8M05_RS02465 (E8M05_02460)	priA	439294..441684 (+)	2391	WP_069789207.1	primosomal protein N'	Machinery gene
E8M05_RS02470 (E8M05_02465)	fmt	441745..442680 (+)	936	WP_069789206.1	methionyl-tRNA formyltransferase	-
E8M05_RS02475 (E8M05_02470)	rsmB	442670..443992 (+)	1323	WP_069789205.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
E8M05_RS02480 (E8M05_02475)	-	444030..444767 (+)	738	WP_003063530.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
E8M05_RS02485 (E8M05_02480)	pknB	444767..446647 (+)	1881	WP_003063531.1	Stk1 family PASTA domain-containing Ser/Thr kinase	Regulator

Sequence

Protein

• Length: 796 a.a.
• Molecular weight: 91135.34 Da
• Isoelectric Point: 7.0645

>NTDB_id=317796 E8M05_RS02465 WP_069789207.1 439294..441684(+) (priA) [Streptococcus pasteurianus strain WUSP067]
MVKIAQVIVDVPLMQTDKPFSYIIPKDVEDQVTIGSRVHVPFGRGNRLLQGFVVGFSDTFDNTVTDLKAISEVLDFEPVL
NVEQLELAEQMRHTVFSYKISILKSMIPNLLNSQYDKRLTPTESLSSEERLALFGEKESRLYSSFTEEEAKKVARLVQAG
KITVDYLAKDKKNIKTEKYYYIQAEKMAAADISNRAKKRLELRDYLLEHPEEGRLSDLHHLFSRDVVKFFVDNQLITVLE
REKKRSDAYFDVATTDFLDLNAEQAAVVEQVTSQIAQESNPFLLEGVTGSGKTEVYLHIIDKVLKLGKTAIVLVPEISLT
PQMTNRFISRFGKQVAIMHSALSDGEKFDEWRKIKSGQARVVVGARSAIFVPIENIGAIIIDEEHEATYKQESNPRYHAR
DVALLRAKYHKAVLLMGSATPSIESRARASRGVYQFLQLTHRANPMAKIPKVEIVDFRDYVGQQEVSNFTPYLLEKIADR
LEKNEQVVLMLNRRGYSSFIMCRDCGYVDECPNCDISLTLHMDTKTMNCHYCGFEKSIPHTCPNCHSRSIRYYGTGTQKA
YDELVEVFSQARILRMDVDTTRQKGAHQRILDKFGNHEADILLGTQMIAKGLDFPNVTLVGVLNADTSLNLPDFRSSERT
FQLLTQVAGRAGRAEKEGEVLIQTYNPQHYAIQLAQKQDYEAFYAYEMGIRRQLAYPPYYFTVGLTLSHKDEQTVVRKSF
ELLQLLRQQLSDKIKILGPTPKPIARTHNLYHYQIIVKYRFEDNLENVLNQILDLTQLPENKDLRLVIDYEPQNFM

Nucleotide

• Length: 2391 bp

>NTDB_id=317796 E8M05_RS02465 WP_069789207.1 439294..441684(+) (priA) [Streptococcus pasteurianus strain WUSP067]
ATGGTAAAAATTGCTCAGGTCATCGTTGATGTCCCACTTATGCAAACAGATAAACCTTTCTCTTACATCATTCCGAAAGA
TGTTGAAGACCAAGTGACCATCGGTTCACGAGTTCATGTCCCTTTTGGACGTGGTAATCGGCTTTTGCAAGGCTTTGTTG
TCGGTTTTTCTGATACTTTTGATAATACTGTGACTGACTTAAAAGCTATTAGTGAAGTTTTGGATTTTGAGCCTGTGTTG
AATGTAGAACAGCTAGAATTGGCAGAGCAGATGCGTCATACCGTGTTTTCTTATAAAATTTCCATTCTGAAATCGATGAT
TCCTAATCTGCTGAATTCGCAGTATGATAAACGTTTAACGCCTACAGAAAGCTTATCTAGCGAAGAACGTTTGGCATTGT
TTGGTGAGAAAGAGAGCCGACTTTATTCGTCATTCACTGAGGAAGAAGCGAAAAAAGTCGCTCGCTTGGTTCAAGCAGGA
AAAATTACCGTTGATTATTTAGCTAAAGATAAGAAAAATATCAAAACGGAAAAATATTATTACATTCAAGCTGAAAAAAT
GGCTGCCGCAGACATCTCCAATCGTGCTAAAAAAAGATTGGAATTACGTGATTATTTGTTAGAGCATCCTGAAGAAGGAA
GACTGTCAGATTTGCATCATCTTTTTTCACGTGATGTTGTCAAATTTTTTGTGGATAATCAGTTGATAACTGTCCTTGAA
AGAGAGAAAAAACGTTCAGACGCTTATTTTGATGTAGCAACAACAGATTTTCTTGATTTAAATGCTGAACAAGCGGCTGT
CGTTGAACAAGTAACTAGCCAAATTGCTCAGGAAAGTAATCCGTTTTTACTGGAAGGTGTGACAGGTTCAGGAAAAACTG
AAGTTTATCTGCATATCATTGACAAGGTTTTGAAACTCGGTAAAACAGCCATTGTCTTGGTGCCTGAAATTTCTTTGACA
CCACAAATGACCAATCGTTTTATTTCACGTTTCGGCAAGCAAGTGGCTATCATGCACTCTGCTTTGTCAGACGGTGAAAA
GTTTGACGAATGGCGAAAAATCAAGTCTGGACAAGCACGTGTCGTTGTTGGGGCACGCTCTGCCATTTTTGTTCCTATTG
AAAATATTGGTGCCATTATCATTGACGAGGAACATGAGGCGACTTACAAGCAAGAATCCAATCCGCGCTACCATGCGCGT
GATGTGGCTTTGCTTCGTGCTAAATACCATAAAGCTGTGTTGTTAATGGGCTCTGCAACACCAAGTATCGAAAGCCGTGC
TCGGGCTAGTCGAGGTGTTTACCAATTCCTACAATTGACACATCGTGCTAATCCTATGGCAAAGATTCCAAAAGTTGAAA
TTGTCGATTTTCGCGATTATGTCGGGCAGCAAGAGGTTAGCAACTTTACTCCGTATCTCTTAGAAAAAATTGCTGACCGC
TTAGAAAAAAATGAGCAAGTCGTGCTCATGCTCAATCGTCGTGGTTACTCTAGTTTTATCATGTGCCGTGATTGTGGCTA
TGTTGATGAATGTCCAAATTGCGACATTTCCTTGACGCTGCATATGGATACCAAAACCATGAATTGTCATTATTGTGGTT
TTGAAAAATCCATTCCACACACTTGTCCGAATTGTCATAGCCGTAGTATTCGGTATTATGGAACAGGAACACAGAAGGCT
TATGATGAACTCGTGGAAGTTTTTTCACAAGCTCGTATTTTACGAATGGACGTTGATACCACACGTCAAAAAGGGGCTCA
TCAACGTATTTTGGATAAGTTTGGCAACCATGAAGCCGATATTTTACTTGGCACACAAATGATTGCTAAGGGACTTGATT
TCCCAAATGTTACTTTGGTAGGAGTTTTAAATGCGGATACGTCTTTAAATTTACCTGATTTTCGTTCTTCTGAACGCACA
TTCCAATTGTTGACACAAGTTGCAGGGCGTGCAGGACGTGCAGAAAAAGAAGGTGAGGTTCTTATCCAGACTTACAATCC
ACAGCACTATGCTATTCAGCTAGCACAAAAGCAAGACTACGAGGCATTTTACGCTTATGAAATGGGAATCCGTAGGCAAT
TAGCTTATCCGCCTTATTATTTCACGGTCGGATTGACCTTATCACATAAAGATGAGCAGACCGTTGTCCGTAAATCATTT
GAACTCTTGCAATTATTGCGCCAGCAATTATCAGATAAAATCAAAATTCTCGGTCCGACACCAAAACCGATTGCCAGAAC
GCATAATCTTTATCATTATCAAATTATCGTTAAATATCGCTTTGAGGACAATTTAGAGAATGTCTTGAACCAGATTTTAG
ATCTGACACAATTACCAGAAAATAAAGATTTACGCTTAGTCATTGACTATGAGCCACAAAATTTCATGTAG

Domains

Predicted by InterProScan.

• Helicase conserved C-terminal domain (Helicase_C) (570-654)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (6-108)

• Primosomal protein N C-terminal domain (PriA_C) (698-792)

• Type III restriction enzyme, res subunit (ResIII) (259-422)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (511-536)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	46.972	100	0.477