Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   CFM83_RS05170 Genome accession   NZ_CP032640
Coordinates   1024851..1025558 (-) Length   235 a.a.
NCBI ID   WP_226474265.1    Uniprot ID   -
Organism   Latilactobacillus sakei strain ZFM229     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1019851..1030558
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFM83_RS05145 (CFM83_05145) rpsO 1020145..1020414 (-) 270 WP_011374766.1 30S ribosomal protein S15 -
  CFM83_RS05150 (CFM83_05150) rpsT 1020693..1020947 (+) 255 WP_011374767.1 30S ribosomal protein S20 -
  CFM83_RS05155 (CFM83_05155) holA 1021016..1022044 (-) 1029 WP_061826985.1 DNA polymerase III subunit delta -
  CFM83_RS05160 (CFM83_05160) comEC 1022041..1024107 (-) 2067 WP_226474266.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  CFM83_RS05165 (CFM83_05165) - 1024305..1024793 (-) 489 WP_011374770.1 ComE operon protein 2 -
  CFM83_RS05170 (CFM83_05170) comEA 1024851..1025558 (-) 708 WP_226474265.1 helix-hairpin-helix domain-containing protein Machinery gene
  CFM83_RS05175 (CFM83_05175) - 1025632..1026678 (-) 1047 WP_061826983.1 SepM family pheromone-processing serine protease -
  CFM83_RS05180 (CFM83_05180) coaD 1026680..1027177 (-) 498 WP_061826982.1 pantetheine-phosphate adenylyltransferase -
  CFM83_RS05185 (CFM83_05185) rsmD 1027190..1027744 (-) 555 WP_011374774.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  CFM83_RS05190 (CFM83_05190) - 1027749..1028039 (-) 291 WP_011374775.1 YlbG family protein -
  CFM83_RS05195 (CFM83_05195) - 1028063..1029151 (-) 1089 WP_061826981.1 CAP-associated domain-containing protein -
  CFM83_RS05200 (CFM83_05200) - 1029162..1030349 (-) 1188 WP_011374777.1 FtsW/RodA/SpoVE family cell cycle protein -

Sequence

Protein


Download         Length: 235 a.a.        Molecular weight: 26122.75 Da        Isoelectric Point: 9.7683

>NTDB_id=317496 CFM83_RS05170 WP_226474265.1 1024851..1025558(-) (comEA) [Latilactobacillus sakei strain ZFM229]
MMWQDYWQRYRRFWWVPCLVLLGIITGGFYWHQTQQKQKVTHNEQLLATSASKIKPATKKTHQAATKKKGGFVDIKGAVA
YPGIYPITVSETRLFEVLKNAGGPTDAADTKRLNLAQKLTDQLIIYIPKKGEVVTDQELIQAPQQIATTSERTPEQASTG
TSANASSASDTAKIDLNQADSTQLQTLNGIGPKKAEQIIAYRDEQGKFKQIEDLQKVGGIGPKTFEQLQSQICVN

Nucleotide


Download         Length: 708 bp        

>NTDB_id=317496 CFM83_RS05170 WP_226474265.1 1024851..1025558(-) (comEA) [Latilactobacillus sakei strain ZFM229]
ATGATGTGGCAAGATTATTGGCAGCGATATCGGCGTTTTTGGTGGGTGCCTTGCTTAGTGCTATTGGGGATCATCACGGG
TGGCTTTTATTGGCATCAAACGCAACAAAAACAAAAAGTAACGCATAATGAGCAACTGCTAGCAACCAGTGCGTCTAAAA
TAAAACCTGCTACTAAGAAAACACACCAAGCGGCAACCAAGAAAAAAGGCGGTTTTGTCGATATAAAAGGTGCTGTGGCT
TATCCGGGAATTTATCCGATCACAGTCTCTGAAACGCGCTTATTTGAGGTGCTCAAAAATGCAGGTGGTCCGACTGATGC
GGCTGATACCAAGCGGCTTAATTTAGCCCAAAAATTAACCGATCAACTGATTATTTATATTCCGAAAAAAGGAGAGGTGG
TTACTGATCAAGAACTCATTCAAGCGCCACAACAAATTGCAACGACCAGTGAGCGTACGCCAGAACAAGCATCAACTGGG
ACAAGTGCGAATGCATCGAGCGCGAGTGACACTGCTAAAATCGATTTGAATCAGGCTGATAGTACGCAGCTTCAGACCCT
CAATGGCATTGGCCCCAAGAAAGCTGAACAAATAATTGCTTATCGTGATGAACAAGGAAAATTTAAACAGATTGAAGATC
TACAAAAAGTCGGCGGGATTGGTCCGAAGACGTTTGAACAATTGCAATCACAAATTTGTGTGAATTAG

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(73-124)

Helix-hairpin-helix motif (HHH_3)

(172-233)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Latilactobacillus sakei subsp. sakei 23K

97.021

100

0.97

Multiple sequence alignment