Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   CFK78_RS08485 Genome accession   NZ_CP032633
Coordinates   1693320..1693832 (-) Length   170 a.a.
NCBI ID   WP_035145013.1    Uniprot ID   A0A9N7J0G1
Organism   Latilactobacillus sakei strain ZFM220     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1688320..1698832
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFK78_RS08455 (CFK78_08455) - 1689246..1689611 (+) 366 WP_011374991.1 DUF805 domain-containing protein -
  CFK78_RS08465 (CFK78_08465) - 1690154..1690612 (-) 459 WP_035145008.1 hypothetical protein -
  CFK78_RS08470 (CFK78_08470) - 1690667..1691863 (-) 1197 WP_011374993.1 acetate/propionate family kinase -
  CFK78_RS08475 (CFK78_08475) - 1691885..1692895 (-) 1011 WP_016265372.1 class I SAM-dependent methyltransferase -
  CFK78_RS08480 (CFK78_08480) - 1693019..1693357 (-) 339 WP_035145011.1 hypothetical protein -
  CFK78_RS08485 (CFK78_08485) comGF 1693320..1693832 (-) 513 WP_035145013.1 competence type IV pilus minor pilin ComGF Machinery gene
  CFK78_RS08490 (CFK78_08490) comGE 1693807..1694112 (-) 306 WP_016265375.1 hypothetical protein Machinery gene
  CFK78_RS08495 (CFK78_08495) comGD 1694099..1694503 (-) 405 WP_235596237.1 hypothetical protein Machinery gene
  CFK78_RS08500 (CFK78_08500) comGC 1694523..1694822 (-) 300 WP_025016383.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  CFK78_RS08505 (CFK78_08505) comGB 1694819..1695826 (-) 1008 WP_061827023.1 type II secretion system F family protein Machinery gene
  CFK78_RS08510 (CFK78_08510) comGA 1695819..1696709 (-) 891 WP_025016380.1 competence type IV pilus ATPase ComGA Machinery gene
  CFK78_RS08515 (CFK78_08515) - 1696826..1697557 (-) 732 WP_011375002.1 YebC/PmpR family DNA-binding transcriptional regulator -
  CFK78_RS08520 (CFK78_08520) - 1697648..1698148 (-) 501 WP_016265379.1 VanZ family protein -
  CFK78_RS08525 (CFK78_08525) - 1698245..1698619 (+) 375 WP_011375004.1 hypothetical protein -

Sequence

Protein


Download         Length: 170 a.a.        Molecular weight: 19689.58 Da        Isoelectric Point: 6.9899

>NTDB_id=317400 CFK78_RS08485 WP_035145013.1 1693320..1693832(-) (comGF) [Latilactobacillus sakei strain ZFM220]
MRLIGRPIKHSAFTLIEMVISMAILILTSQLLVATVGVLQKAIKVTQQQQNTINWHISTIQLDQFLADAYFTHHQDQDGS
KITFRKPDTNQSGEEAETVYYLKRMKNEMVVVSQLNGYMPLLGNLKQLQLDYQRPFVQIDATFTDQSRYQHEVFLEEQDE
QAKKKTIKTD

Nucleotide


Download         Length: 513 bp        

>NTDB_id=317400 CFK78_RS08485 WP_035145013.1 1693320..1693832(-) (comGF) [Latilactobacillus sakei strain ZFM220]
TTGCGATTAATTGGACGACCGATTAAGCATTCAGCGTTCACGCTAATTGAAATGGTGATTTCAATGGCGATTCTAATCCT
GACGAGTCAGTTACTGGTGGCAACGGTGGGTGTTTTACAAAAGGCAATTAAAGTCACGCAACAACAGCAAAATACAATCA
ATTGGCATATCAGTACGATTCAGTTAGATCAGTTTTTAGCCGATGCGTATTTTACACATCATCAGGATCAAGATGGCTCC
AAAATCACTTTTCGAAAACCAGACACTAATCAGTCAGGGGAGGAAGCGGAGACTGTTTACTATTTAAAAAGGATGAAAAA
TGAAATGGTTGTTGTCAGCCAATTAAATGGGTATATGCCATTATTAGGCAACCTCAAGCAACTTCAACTTGACTACCAGC
GACCGTTTGTTCAAATCGATGCGACTTTTACGGATCAGAGTCGCTACCAACACGAAGTTTTCTTGGAGGAACAAGATGAA
CAGGCTAAAAAAAAGACGATTAAAACCGATTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(40-149)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Latilactobacillus sakei subsp. sakei 23K

98.235

100

0.982

Multiple sequence alignment