Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   OEA_RS03310 Genome accession   NZ_CP032527
Coordinates   646052..648394 (+) Length   780 a.a.
NCBI ID   WP_016765631.1    Uniprot ID   -
Organism   Priestia megaterium NCT-2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 641052..653394
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OEA_RS03295 (OEA_04710) spoVB 641568..643118 (-) 1551 WP_034669037.1 stage V sporulation protein B -
  OEA_RS03300 (OEA_04715) - 643243..643536 (+) 294 WP_016765633.1 post-transcriptional regulator -
  OEA_RS03305 (OEA_04720) secDF 643674..645911 (+) 2238 WP_016765632.1 protein translocase subunit SecDF -
  OEA_RS03310 (OEA_04725) recJ 646052..648394 (+) 2343 WP_016765631.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  OEA_RS03315 (OEA_04730) - 648410..648922 (+) 513 WP_013059309.1 adenine phosphoribosyltransferase -
  OEA_RS03320 (OEA_04735) - 649108..651306 (+) 2199 WP_013059308.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  OEA_RS03325 (OEA_04740) dtd 651322..651783 (+) 462 WP_016765630.1 D-aminoacyl-tRNA deacylase -
  OEA_RS03330 (OEA_04745) - 651774..652676 (-) 903 WP_016765629.1 N-acetylmuramoyl-L-alanine amidase -
  OEA_RS03335 (OEA_04750) - 652825..653001 (+) 177 WP_013059305.1 hypothetical protein -

Sequence

Protein


Download         Length: 780 a.a.        Molecular weight: 87980.59 Da        Isoelectric Point: 4.6748

>NTDB_id=316760 OEA_RS03310 WP_016765631.1 646052..648394(+) (recJ) [Priestia megaterium NCT-2]
MLQSKKRWNVQECDEQLVNEFVENLNITPLVASLLLNRGMTTVEAAREFLHVDAQTFHDPFLLHDMDKAVARIQQAIDHN
EKIMIYGDYDADGVSSTSVMLSALQQLGADTDFYIPNRFTEGYGPNKAAFDKINTGGYQLVITVDTGISAVEETAYAREL
DFDLIITDHHEPGPILPDAYAIIHPKLPGSTYPFKELAGVGVAFKLAHALLGELPEHLLELAVIGTIADLVPLVDENRLI
ARKGLDYLKLTKRTGIEALLSVCGVKREEITEDTIGFAMAPRINAVGRLQDADPAVHLLMTTNAEEAKGLAKEIDALNKV
RQQIVNDITEEAVEFVENMYPPDENSVLIVEGEGWNAGVVGIVASRLVEKFYRPTIVLSIDSENRIAKGSARSIEGFDLF
ANLSKCRDILPHFGGHPMAAGMTLNSDDVEELRTRLNEQASNVLTEQDFIPITSVDTVCTTEEITLENIEQMSALAPFGM
HNPKPKVLIKDVHTTSMRKIGANKNHLKLVFEDSDVSVDAVGFGLGYILDEVSPIAKVSLVGELSINEWNNMRKPQLMVE
DISVDEWQMFDYRSAVDVKRFFQQVFTDQMKVVAFHASTIDKYKSLLPMEHVVLIQDEEQAKECMFAGEQIVLLDLPPLQ
KWLECLFVQGIPSRIYAIFLTEEDHYFDTIPTRDHFKWFYGFLAKKGPFDLKRYKEDLAIHKGWSKETIDFMAKVFFELE
FVTINNGLISLVKNSAKRDLIESATYRQKQQQIEIEKNFIYTSYVQLKNQFQDIFKRSIE

Nucleotide


Download         Length: 2343 bp        

>NTDB_id=316760 OEA_RS03310 WP_016765631.1 646052..648394(+) (recJ) [Priestia megaterium NCT-2]
ATGTTACAGTCAAAAAAACGTTGGAATGTTCAAGAATGTGATGAACAACTTGTTAATGAATTTGTAGAAAACCTCAATAT
TACACCTCTAGTAGCTTCGTTATTACTTAATAGAGGCATGACAACAGTAGAAGCGGCACGTGAGTTTTTACACGTTGATG
CACAAACGTTCCACGATCCGTTTTTATTGCACGATATGGACAAAGCGGTAGCTCGTATTCAGCAAGCAATAGATCATAAT
GAAAAAATTATGATCTATGGAGATTACGATGCGGACGGCGTCAGCAGTACTTCTGTTATGTTATCTGCTTTACAGCAGCT
CGGCGCTGATACTGATTTTTATATTCCTAATCGCTTTACAGAAGGTTATGGGCCGAATAAGGCAGCATTTGATAAAATAA
ACACAGGCGGCTATCAGCTGGTGATTACGGTAGACACAGGTATTTCGGCAGTCGAAGAAACGGCGTATGCACGTGAATTA
GATTTTGATTTAATTATTACGGATCACCACGAGCCGGGTCCGATTTTACCGGATGCGTATGCAATTATTCATCCAAAGCT
GCCGGGAAGTACATATCCATTTAAAGAACTGGCGGGTGTAGGAGTGGCTTTTAAACTCGCTCATGCGCTGTTAGGGGAGC
TTCCGGAACATTTGTTAGAACTAGCAGTGATTGGGACGATTGCTGACTTGGTTCCTTTAGTCGACGAAAACCGCTTAATT
GCTCGCAAAGGTCTTGACTATCTCAAGCTCACGAAGCGCACTGGAATTGAAGCGCTGCTGAGCGTATGTGGAGTAAAACG
CGAAGAAATTACTGAAGATACAATTGGATTTGCTATGGCTCCTCGCATTAATGCAGTAGGACGTCTTCAAGATGCTGATC
CAGCTGTTCACTTGCTTATGACAACAAATGCTGAAGAAGCAAAAGGTTTAGCTAAAGAAATTGATGCGTTAAACAAGGTA
AGGCAGCAAATCGTAAACGATATTACGGAAGAAGCCGTTGAATTTGTTGAAAATATGTATCCTCCGGATGAAAACAGCGT
TCTTATTGTAGAGGGAGAAGGATGGAATGCAGGTGTTGTTGGAATTGTCGCTTCTAGGCTCGTAGAAAAGTTCTACAGAC
CAACAATTGTACTAAGTATTGACTCTGAAAACCGTATTGCCAAAGGTTCAGCAAGAAGTATCGAAGGCTTTGACTTATTC
GCGAACTTATCTAAATGCCGTGACATTTTGCCGCATTTTGGAGGGCATCCAATGGCAGCCGGGATGACGTTAAACAGCGA
TGATGTAGAAGAGCTGCGCACACGTCTTAATGAACAAGCAAGCAATGTGTTAACCGAACAGGATTTTATTCCAATTACAT
CAGTTGATACAGTATGCACGACAGAGGAAATTACGCTGGAAAACATTGAACAAATGAGTGCGCTTGCTCCATTTGGTATG
CATAACCCGAAGCCAAAAGTTCTCATTAAAGATGTACATACAACATCTATGCGGAAAATTGGCGCTAATAAAAATCATTT
AAAACTGGTGTTTGAAGACAGCGATGTATCGGTTGATGCAGTAGGGTTTGGGTTAGGCTATATATTAGATGAAGTATCTC
CTATAGCTAAAGTATCGTTAGTAGGTGAACTATCTATTAATGAGTGGAATAATATGCGCAAGCCGCAGCTGATGGTAGAA
GATATTTCTGTAGATGAATGGCAGATGTTTGATTATCGCAGTGCAGTTGATGTCAAACGTTTCTTTCAGCAGGTATTTAC
GGATCAAATGAAAGTTGTTGCTTTTCACGCTTCAACAATTGATAAATACAAGTCGCTGCTGCCGATGGAGCATGTTGTTC
TTATTCAAGATGAAGAACAGGCAAAAGAATGTATGTTTGCAGGGGAACAAATCGTTTTATTAGATTTGCCTCCTTTACAG
AAGTGGCTTGAATGTTTGTTTGTCCAAGGTATCCCTTCTAGAATATACGCTATTTTCTTAACGGAAGAAGACCATTATTT
TGATACAATCCCAACTCGCGATCATTTTAAATGGTTTTACGGCTTTTTAGCGAAAAAGGGTCCATTTGATTTAAAACGCT
ACAAAGAAGATTTAGCGATTCATAAGGGCTGGTCAAAAGAAACGATTGATTTTATGGCAAAGGTGTTTTTTGAGTTAGAA
TTTGTTACAATAAACAATGGCTTAATTTCGTTAGTGAAAAATTCTGCGAAACGTGATTTAATAGAATCTGCGACTTATCG
CCAAAAGCAGCAGCAGATTGAAATAGAAAAAAACTTTATTTATACGTCATATGTACAGCTTAAAAATCAATTTCAAGATA
TTTTTAAGCGTTCGATTGAATGA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(305-439)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-770)

DHH family (DHH)

(82-226)

RecJ OB domain (RecJ_OB)

(455-561)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

55.128

100

0.551

Multiple sequence alignment