Detailed information NTG0316438

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: E5Q05_RS03950
• Genome accession: NZ_CP038975
• Coordinates: 784508..785683 (+)
• Length: 391 a.a.
• NCBI ID: WP_010887416.1
• Uniprot ID: Q9RW99
• Organism: Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 strain ATCC 13939 substr. S1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 779508..790683

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E5Q05_RS03930	-	780226..781554 (+)	1329	WP_227086001.1	PQQ-binding-like beta-propeller repeat protein	-
E5Q05_RS03935	-	781563..782060 (+)	498	WP_010887413.1	short-chain dehydrogenase	-
E5Q05_RS03940	-	782088..783389 (+)	1302	WP_010887414.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
E5Q05_RS03945	-	783424..784227 (+)	804	WP_010887415.1	hypothetical protein	-
E5Q05_RS03950	pilM	784508..785683 (+)	1176	WP_010887416.1	type IV pilus biogenesis protein PilM	Machinery gene
E5Q05_RS03955	-	785676..786392 (+)	717	WP_010887417.1	hypothetical protein	-
E5Q05_RS03960	-	786389..787048 (+)	660	WP_010887418.1	type 4a pilus biogenesis protein PilO	-
E5Q05_RS03965	-	787045..788952 (+)	1908	WP_010887419.1	hypothetical protein	-

Sequence

Protein

• Length: 391 a.a.
• Molecular weight: 42173.15 Da
• Isoelectric Point: 4.6785

>NTDB_id=316438 E5Q05_RS03950 WP_010887416.1 784508..785683(+) (pilM) [Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 strain ATCC 13939 substr. S1]
MTSLINRFRSRSAAIGVEIGTSTIKVVALKAGAPPSLQHAVMVPTPIGSMRDGLVVEPQAVASELKSLLAEHRITTRHAV
TAVPNQVAVTRNIMVPRMDRKDLQSAIRWEAERYIPYPIDEVTLDFDLLDDPANVPEDGQMEVVIAAAPTEAVHRQIEVL
RLAGLEPTVVDLKSFAALRALRGNLLGEHLTKSTLTGTNYTEAGEVALVMEIGASSSVINLVRGDRILMTRNINVSADDF
TTALQKAFDLDFAAAEDVKLGYATATTPTEDEEDLLNFDLSREQYSPARVFEVVRPVLGDLITEIRRSLEFYRVQSGDVV
IDRTFLAGGGAKLRGLAAAIGDALGFGVEVASPWLTVQTDQAGVDTGYLQTNAPEFTVPLGLALRGVMGRG

Nucleotide

• Length: 1176 bp

>NTDB_id=316438 E5Q05_RS03950 WP_010887416.1 784508..785683(+) (pilM) [Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 strain ATCC 13939 substr. S1]
ATGACGAGCCTCATTAACCGTTTTCGCAGTCGTTCCGCCGCCATCGGCGTGGAGATCGGCACCAGCACCATCAAGGTGGT
GGCCCTTAAGGCAGGGGCCCCGCCTTCCCTGCAACACGCGGTGATGGTCCCCACCCCCATCGGCAGCATGCGTGACGGTC
TGGTCGTCGAACCCCAGGCAGTGGCGAGCGAACTCAAAAGCCTGCTGGCCGAGCACCGCATCACCACCCGCCACGCCGTC
ACCGCCGTGCCCAATCAGGTGGCCGTGACCCGCAACATCATGGTGCCGCGCATGGACCGCAAGGACCTGCAAAGCGCCAT
TCGCTGGGAAGCCGAGCGCTACATCCCTTACCCCATCGACGAGGTCACGCTCGACTTCGACCTGCTCGACGACCCCGCCA
ACGTGCCCGAGGACGGGCAGATGGAAGTGGTCATCGCGGCGGCGCCCACCGAGGCCGTGCACCGTCAGATCGAGGTGCTG
CGCCTCGCGGGCCTCGAACCCACCGTGGTGGACCTCAAGAGCTTCGCGGCGCTGCGGGCGCTACGCGGCAACCTGCTCGG
CGAGCACCTCACCAAGAGCACCCTAACCGGCACCAACTACACCGAGGCCGGCGAGGTGGCGCTGGTGATGGAAATCGGCG
CGAGCAGCAGCGTCATCAATCTGGTGCGCGGCGACCGCATCCTGATGACCCGCAACATCAACGTGTCCGCCGACGATTTC
ACCACTGCGCTGCAAAAGGCCTTCGACCTCGACTTTGCCGCCGCCGAGGATGTCAAGCTCGGTTACGCCACCGCCACCAC
CCCCACCGAGGACGAGGAAGACCTGCTCAACTTCGACCTCAGCCGCGAGCAGTACAGCCCGGCGCGCGTGTTCGAAGTGG
TGCGCCCGGTGCTCGGCGACCTGATCACCGAAATCCGCCGCTCGCTGGAGTTCTACCGCGTGCAGAGCGGCGACGTGGTC
ATCGACCGGACCTTCCTCGCCGGGGGCGGCGCCAAGCTGCGCGGCCTGGCGGCGGCCATCGGGGACGCGCTGGGCTTCGG
GGTCGAAGTCGCGTCGCCCTGGCTGACGGTGCAGACCGACCAGGCGGGCGTGGACACCGGCTACCTCCAGACCAACGCCC
CCGAATTCACCGTGCCGCTGGGACTGGCGCTGCGGGGGGTGATGGGCCGTGGTTGA

Domains

Predicted by InterProScan.

• Type IV pilus assembly protein PilM; (PilM_2) (16-260)

• Type IV pilus assembly protein PilM; (PilM_2) (291-386)

3D structure

• AlphaFold DB: Q9RW99

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Thermus thermophilus HB27	46.512	98.977	0.46