Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   D5S10_RS04430 Genome accession   NZ_CP032456
Coordinates   1008815..1010509 (-) Length   564 a.a.
NCBI ID   WP_031597287.1    Uniprot ID   A0A554GP76
Organism   Pseudomonas savastanoi strain CAS03     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1003815..1015509
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D5S10_RS04410 (D5S10_04830) yacG 1005895..1006104 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  D5S10_RS04415 (D5S10_04835) coaE 1006101..1006724 (-) 624 WP_016981135.1 dephospho-CoA kinase -
  D5S10_RS04420 (D5S10_04840) pilD 1006721..1007593 (-) 873 WP_016981136.1 A24 family peptidase Machinery gene
  D5S10_RS04425 (D5S10_04845) pilC 1007595..1008812 (-) 1218 WP_016981137.1 type II secretion system F family protein Machinery gene
  D5S10_RS04430 (D5S10_04850) pilB 1008815..1010509 (-) 1695 WP_031597287.1 type IV-A pilus assembly ATPase PilB Machinery gene
  D5S10_RS04435 (D5S10_04855) - 1010735..1011130 (+) 396 WP_029240905.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  D5S10_RS04440 (D5S10_04860) - 1011400..1012596 (+) 1197 WP_371911670.1 O-antigen ligase family protein -
  D5S10_RS04445 (D5S10_04865) - 1012732..1014018 (+) 1287 WP_016981140.1 lipopolysaccharide biosynthesis protein -
  D5S10_RS04450 (D5S10_04870) - 1014068..1014613 (-) 546 WP_016981141.1 DUF1523 family protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62542.83 Da        Isoelectric Point: 6.1206

>NTDB_id=315991 D5S10_RS04430 WP_031597287.1 1008815..1010509(-) (pilB) [Pseudomonas savastanoi strain CAS03]
MSDAVLTGLAKQLVLAELLTETAAQQAYQQARRDKISLVSYLVQNKLVKSLTLAEMASDQFGVPFMDLASLDKESQPKGL
VSEKLIRQHHALPLWRRGNKLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTIAIDRFFDTESGLGELADIDLGLD
IEPASDKETSLATQSDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKNKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPDQKALYMEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRETLIQEGFPEEK
IGTFKIYGPVGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=315991 D5S10_RS04430 WP_031597287.1 1008815..1010509(-) (pilB) [Pseudomonas savastanoi strain CAS03]
ATGTCTGATGCTGTCCTCACCGGTTTGGCCAAACAACTGGTCCTGGCCGAGCTGCTTACCGAAACTGCTGCGCAGCAAGC
TTATCAGCAGGCGCGCCGCGACAAGATTTCATTGGTAAGTTATCTGGTCCAGAACAAACTGGTCAAAAGCCTGACGCTGG
CAGAAATGGCTTCTGATCAGTTCGGCGTTCCTTTCATGGACCTTGCCAGCCTGGACAAGGAAAGCCAGCCCAAAGGCCTT
GTGAGTGAAAAGCTGATTCGTCAGCACCATGCACTGCCGCTGTGGCGTCGTGGTAATAAGCTGTTTATAGGCATCTCAGA
CCCTACCAACCACCAGGCAGTCACTGACATACAATTCAGTACCGGCCTGAACACTGAAGCGATTCTGGTTGAAGATGACA
AGCTGACCATTGCCATAGACCGTTTTTTTGACACTGAAAGCGGCCTTGGCGAGTTGGCAGACATTGATTTGGGCCTGGAC
ATCGAGCCTGCGAGTGACAAGGAAACCTCACTGGCCACTCAGAGTGATGCCGATGATGCCCCGGTCGTGCGCTTCGTTAA
CAAGATGCTGATGGACGCGATACGGCTCGGCTCGTCCGACCTGCATTTTGAACCTTACGAAAAGATCTTCCGAGTGCGGT
TGCGTACCGACGGCATTTTGCATGAGGTGGCCAGACCACCGATCCATCTGGCCAACCGTATTGCCGCGCGCCTGAAGGTC
ATGGCCAGCCTCGATATCTCTGAGCGGCGTAAACCGCAGGACGGCCGGGTCAAGCTGCGAGTGTCAAAAAACAAAGCTAT
CGACTTCCGTATGAATACGCTGCCGACACTGTGGGGCGAGAAGATCGTGATGCGGATTCTCGATCCGACCAGCGCACAGA
TGGGCATTGACGCGCTGGGCTATGAGCCGGACCAAAAAGCTTTGTATATGGAGGCCTTGAAACAGCCACAAGGCATGATT
CTTGTAACCGGTCCCACTGGCTCGGGTAAAACCGTTTCCCTATACACTGGCCTGAACATTCTCAACACTGTGGACATCAA
CATTTCCACAGCTGAAGACCCGGTAGAGATCAACCTGGAAGGCATCAATCAGGTCAACGTCAACCCGCGTCAGGGGTTGG
ACTTTTCTCAGGCGTTGCGCGCATTCCTGCGTCAAGATCCCGACGTAATCATGGTCGGCGAGATACGAGACCTGGAAACA
GCCGAAATCGCCATTAAAGCCTCGCAGACCGGCCACATGGTGTTATCTACTCTGCATACTAACAGCGCTGCAGAAACGCT
GACTCGCTTGCATCATATGGGTGTCGCAGCGTTCAACATCGCTACGGCGATTAACCTCATCATTGCCCAGCGGCTGGCGC
GCAAGCTATGCAGCCATTGCAAGAAAGAGCTCGACATACCCCGGGAAACGCTCATCCAGGAGGGGTTTCCAGAGGAGAAG
ATCGGTACGTTTAAAATCTATGGCCCAGTAGGTTGTGAGCATTGCAACGGCGGCTACAGGGGCAGGGTCGGCATTTACGA
AGTAGTCAAGAAAACACCAGAACTGGAACGCATCATCATGGAAGAAGGCAATTCGCTGGAGATTTCCAGGCAGATGCGCA
AGGACGGCTTCAATGATTTGAGGACCTCCGGGCTGTCGAAGGCCATGCAAGGTATTACCAGCCTTGAAGAAGTCAACCGC
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A554GP76

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.865

100

0.589
  pilB Acinetobacter baylyi ADP1

57.295

99.645

0.571
  pilB Legionella pneumophila strain ERS1305867

55.83

100

0.56
  pilB Vibrio cholerae strain A1552

50.98

99.468

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

50.722

98.227

0.498
  pilB Vibrio campbellii strain DS40M4

49.468

100

0.495
  pilF Neisseria gonorrhoeae MS11

48.043

99.645

0.479
  pilF Thermus thermophilus HB27

39.223

100

0.394
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.279

93.44

0.367

Multiple sequence alignment