Detailed information NTG0315701

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: E5E91_RS03940
• Genome accession: NZ_CP038663
• Coordinates: 783182..784357 (+)
• Length: 391 a.a.
• NCBI ID: WP_010887416.1
• Uniprot ID: Q9RW99
• Organism: Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 strain ATCC 13939
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 778182..789357

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E5E91_RS03920 (E5E91_03980)	-	778900..780228 (+)	1329	WP_227086001.1	PQQ-binding-like beta-propeller repeat protein	-
E5E91_RS03925 (E5E91_03985)	-	780237..780734 (+)	498	WP_010887413.1	short-chain dehydrogenase	-
E5E91_RS03930 (E5E91_03990)	-	780762..782063 (+)	1302	WP_010887414.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
E5E91_RS03935 (E5E91_03995)	-	782098..782901 (+)	804	WP_010887415.1	hypothetical protein	-
E5E91_RS03940 (E5E91_04000)	pilM	783182..784357 (+)	1176	WP_010887416.1	type IV pilus biogenesis protein PilM	Machinery gene
E5E91_RS03945 (E5E91_04005)	-	784350..785066 (+)	717	WP_010887417.1	hypothetical protein	-
E5E91_RS03950 (E5E91_04010)	-	785063..785722 (+)	660	WP_010887418.1	type 4a pilus biogenesis protein PilO	-
E5E91_RS03955 (E5E91_04015)	-	785719..787626 (+)	1908	WP_010887419.1	hypothetical protein	-

Sequence

Protein

• Length: 391 a.a.
• Molecular weight: 42173.15 Da
• Isoelectric Point: 4.6785

>NTDB_id=315701 E5E91_RS03940 WP_010887416.1 783182..784357(+) (pilM) [Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 strain ATCC 13939]
MTSLINRFRSRSAAIGVEIGTSTIKVVALKAGAPPSLQHAVMVPTPIGSMRDGLVVEPQAVASELKSLLAEHRITTRHAV
TAVPNQVAVTRNIMVPRMDRKDLQSAIRWEAERYIPYPIDEVTLDFDLLDDPANVPEDGQMEVVIAAAPTEAVHRQIEVL
RLAGLEPTVVDLKSFAALRALRGNLLGEHLTKSTLTGTNYTEAGEVALVMEIGASSSVINLVRGDRILMTRNINVSADDF
TTALQKAFDLDFAAAEDVKLGYATATTPTEDEEDLLNFDLSREQYSPARVFEVVRPVLGDLITEIRRSLEFYRVQSGDVV
IDRTFLAGGGAKLRGLAAAIGDALGFGVEVASPWLTVQTDQAGVDTGYLQTNAPEFTVPLGLALRGVMGRG

Nucleotide

• Length: 1176 bp

>NTDB_id=315701 E5E91_RS03940 WP_010887416.1 783182..784357(+) (pilM) [Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 strain ATCC 13939]
ATGACGAGCCTCATTAACCGTTTTCGCAGTCGTTCCGCCGCCATCGGCGTGGAGATCGGCACCAGCACCATCAAGGTGGT
GGCCCTTAAGGCAGGGGCCCCGCCTTCCCTGCAACACGCGGTGATGGTCCCCACCCCCATCGGCAGCATGCGTGACGGTC
TGGTCGTCGAACCCCAGGCAGTGGCGAGCGAACTCAAAAGCCTGCTGGCCGAGCACCGCATCACCACCCGCCACGCCGTC
ACCGCCGTGCCCAATCAGGTGGCCGTGACCCGCAACATCATGGTGCCGCGCATGGACCGCAAGGACCTGCAAAGCGCCAT
TCGCTGGGAAGCCGAGCGCTACATCCCTTACCCCATCGACGAGGTCACGCTCGACTTCGACCTGCTCGACGACCCCGCCA
ACGTGCCCGAGGACGGGCAGATGGAAGTGGTCATCGCGGCGGCGCCCACCGAGGCCGTGCACCGTCAGATCGAGGTGCTG
CGCCTCGCGGGCCTCGAACCCACCGTGGTGGACCTCAAGAGCTTCGCGGCGCTGCGGGCGCTACGCGGCAACCTGCTCGG
CGAGCACCTCACCAAGAGCACCCTAACCGGCACCAACTACACCGAGGCCGGCGAGGTGGCGCTGGTGATGGAAATCGGCG
CGAGCAGCAGCGTCATCAATCTGGTGCGCGGCGACCGCATCCTGATGACCCGCAACATCAACGTGTCCGCCGACGATTTC
ACCACTGCGCTGCAAAAGGCCTTCGACCTCGACTTTGCCGCCGCCGAGGATGTCAAGCTCGGTTACGCCACCGCCACCAC
CCCCACCGAGGACGAGGAAGACCTGCTCAACTTCGACCTCAGCCGCGAGCAGTACAGCCCGGCGCGCGTGTTCGAAGTGG
TGCGCCCGGTGCTCGGCGACCTGATCACCGAAATCCGCCGCTCGCTGGAGTTCTACCGCGTGCAGAGCGGCGACGTGGTC
ATCGACCGGACCTTCCTCGCCGGGGGCGGCGCCAAGCTGCGCGGCCTGGCGGCGGCCATCGGGGACGCGCTGGGCTTCGG
GGTCGAAGTCGCGTCGCCCTGGCTGACGGTGCAGACCGACCAGGCGGGCGTGGACACCGGCTACCTCCAGACCAACGCCC
CCGAATTCACCGTGCCGCTGGGACTGGCGCTGCGGGGGGTGATGGGCCGTGGTTGA

Domains

Predicted by InterProScan.

• Type IV pilus assembly protein PilM; (PilM_2) (16-260)

• Type IV pilus assembly protein PilM; (PilM_2) (291-386)

3D structure

• AlphaFold DB: Q9RW99

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Thermus thermophilus HB27	46.512	98.977	0.46