Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   D4N37_RS27450 Genome accession   NZ_CP032353
Coordinates   6036093..6037310 (+) Length   405 a.a.
NCBI ID   WP_011063521.1    Uniprot ID   Q4K5X3
Organism   Pseudomonas protegens strain pf5-k2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6031093..6042310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D4N37_RS27435 (D4N37_27405) - 6031633..6034551 (+) 2919 WP_011063518.1 DUF748 domain-containing protein -
  D4N37_RS27440 (D4N37_27410) - 6034762..6035118 (-) 357 WP_011063519.1 BON domain-containing protein -
  D4N37_RS27445 (D4N37_27415) - 6035347..6035763 (-) 417 WP_011063520.1 pilin -
  D4N37_RS27450 (D4N37_27420) pilC 6036093..6037310 (+) 1218 WP_011063521.1 type II secretion system F family protein Machinery gene
  D4N37_RS27455 (D4N37_27425) pilD 6037313..6038185 (+) 873 WP_011063522.1 A24 family peptidase Machinery gene
  D4N37_RS27460 (D4N37_27430) coaE 6038182..6038805 (+) 624 WP_011063523.1 dephospho-CoA kinase -
  D4N37_RS27465 (D4N37_27435) yacG 6038802..6039005 (+) 204 WP_011063524.1 DNA gyrase inhibitor YacG -
  D4N37_RS27470 (D4N37_27440) - 6039025..6039240 (-) 216 WP_011063525.1 hypothetical protein -
  D4N37_RS27475 (D4N37_27445) - 6039304..6039993 (-) 690 WP_011063526.1 energy-coupling factor ABC transporter permease -
  D4N37_RS27480 (D4N37_27450) - 6039990..6040457 (-) 468 WP_026019989.1 hypothetical protein -
  D4N37_RS27485 (D4N37_27455) - 6040601..6041230 (+) 630 WP_011063528.1 DUF1780 domain-containing protein -
  D4N37_RS27490 (D4N37_27460) - 6041420..6041593 (+) 174 WP_015637073.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43829.53 Da        Isoelectric Point: 10.5933

>NTDB_id=315075 D4N37_RS27450 WP_011063521.1 6036093..6037310(+) (pilC) [Pseudomonas protegens strain pf5-k2]
MAVKAPATSVYLWHGTDSQGRPLTGELSAATPALIRAQLRRQGITPTRVRRKPKGWLRLHQRVGARDIALLTRQLATLMQ
AGVPLLQSFDIIAESFEHLQMRQLLASLKRDIEAGSSLAAALRRQPRHFDPLYCNLVDAGEQAGALDTLLERVATYKEKS
ENLKARLKKAMVYPLAVIGVAVVVSSILLLKVVPQFQSLFAGFNAELPLLTQWVIALSHFIQQQGALLLAAGVLGGLAAR
LAYRRSPSLRDRLDVGLLHMPLAGPLLHKSAVARYARTLATTFAAGVPLVQALDSVAGASGNPVFRQAVQRIRQQVAAGM
QLNFAMRASGVFPTMAIQMTAIGEESGTLDHLLEKVAIHYEVEVDNLVDSLTSLMEPLIMVVLGGIVGALVVAMYLPIFQ
LGTAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=315075 D4N37_RS27450 WP_011063521.1 6036093..6037310(+) (pilC) [Pseudomonas protegens strain pf5-k2]
ATGGCGGTAAAAGCACCCGCGACCAGTGTCTATCTCTGGCACGGCACCGACAGCCAGGGCCGCCCCCTCACCGGAGAACT
CAGCGCCGCGACCCCGGCCCTGATCAGGGCGCAATTGCGCAGACAGGGCATCACCCCAACCCGAGTCCGACGCAAACCCA
AGGGCTGGCTGCGCCTGCATCAGCGAGTAGGGGCACGGGACATTGCCCTGCTCACCCGGCAGTTGGCCACGCTGATGCAG
GCCGGGGTGCCGCTGCTGCAATCCTTCGACATCATTGCCGAAAGTTTCGAGCATCTGCAGATGCGCCAACTGCTGGCCAG
CCTCAAGCGCGACATCGAGGCCGGCTCCAGCCTGGCCGCGGCATTGCGCAGACAACCGCGCCACTTCGACCCGCTGTACT
GCAACCTGGTGGACGCCGGCGAACAGGCCGGCGCCCTGGATACCCTGCTGGAGAGAGTCGCCACCTATAAGGAAAAGAGC
GAGAACCTGAAGGCGCGGCTGAAGAAGGCCATGGTCTATCCCTTGGCGGTGATCGGCGTGGCGGTCGTGGTCAGCAGCAT
CCTGCTGCTCAAGGTGGTCCCCCAGTTCCAGAGCCTGTTCGCCGGTTTCAATGCCGAGCTGCCACTGCTCACCCAATGGG
TCATCGCTCTGTCACATTTCATCCAGCAGCAGGGGGCGCTGCTGCTGGCTGCCGGTGTGCTGGGTGGGCTCGCAGCGCGC
CTGGCCTATCGGCGTTCCCCGAGCTTGCGTGACCGCCTGGATGTCGGCCTTTTGCACATGCCCCTGGCCGGGCCACTGCT
GCACAAATCCGCGGTGGCCCGCTACGCACGGACCCTGGCCACCACCTTCGCCGCCGGCGTTCCCCTGGTGCAGGCCCTGG
ATTCAGTGGCCGGAGCCAGCGGCAACCCTGTCTTCAGGCAAGCGGTGCAACGGATCCGCCAGCAGGTCGCCGCCGGCATG
CAATTGAACTTTGCCATGCGCGCCAGCGGCGTCTTTCCGACCATGGCGATCCAGATGACCGCCATCGGCGAAGAGTCCGG
AACCCTGGATCATCTGCTGGAAAAAGTCGCGATCCATTACGAGGTCGAGGTGGACAACCTGGTGGACAGCCTGACCAGCC
TCATGGAGCCGTTGATCATGGTGGTCCTGGGGGGTATCGTCGGCGCCCTGGTGGTTGCCATGTACCTGCCTATCTTCCAA
CTCGGTACAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q4K5X3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

58.519

100

0.585
  pilC Acinetobacter baylyi ADP1

52.812

100

0.533
  pilC Acinetobacter baumannii D1279779

52.941

100

0.533
  pilC Legionella pneumophila strain ERS1305867

47.059

100

0.474
  pilG Neisseria meningitidis 44/76-A

42.145

99.012

0.417
  pilG Neisseria gonorrhoeae MS11

42.04

99.259

0.417
  pilC Vibrio cholerae strain A1552

42.602

96.79

0.412
  pilC Vibrio campbellii strain DS40M4

41.294

99.259

0.41
  pilC Thermus thermophilus HB27

38

98.765

0.375

Multiple sequence alignment