Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   D1367_RS02210 Genome accession   NZ_CP031941
Coordinates   465680..466786 (-) Length   368 a.a.
NCBI ID   WP_118171041.1    Uniprot ID   -
Organism   Nostoc sphaeroides strain Kutzing En     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 460680..471786
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1367_RS02190 - 460980..461222 (-) 243 WP_118162337.1 hypothetical protein -
  D1367_RS02195 - 461385..463832 (-) 2448 WP_118162339.1 type IV pilus secretin family protein -
  D1367_RS02200 - 464126..464902 (-) 777 WP_118162342.1 pilus assembly protein PilO -
  D1367_RS02205 - 464899..465675 (-) 777 WP_118162345.1 PilN domain-containing protein -
  D1367_RS02210 pilM 465680..466786 (-) 1107 WP_118171041.1 type IV pilus assembly protein PilM Machinery gene
  D1367_RS02215 - 467054..468358 (+) 1305 WP_118162348.1 ABC transporter substrate-binding protein -
  D1367_RS02220 - 468445..470913 (-) 2469 WP_118162350.1 beta strand repeat-containing protein -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 40454.58 Da        Isoelectric Point: 4.4661

>NTDB_id=312833 D1367_RS02210 WP_118171041.1 465680..466786(-) (pilM) [Nostoc sphaeroides strain Kutzing En]
MVKSFKSLFSKSKKGVGIELAPERVNVVQLRKQGQGLKLETFTSVAVPEGVVTDGQITDPAAMAQLIQQALTESKIKTSR
VATGVPGRDSIVRIIPVPAELDDKELREMVLNHEAGLYLPYPREEADVDYQKLGYKMDEDGIEKVEVLLVATRKEITDTY
ISTFEQAGLQIDILEINSFALIRTIREQLRQFGPQEAAVLVDIEFDSTEIAIIVNGVPQFSRTVPIGTYQMQMALARAMS
LPTSRDMELLHGMVIPPTSLDGGKTGVTEINPGMAAILRVLGELTDELRRSIDFYLNQTENMEVAQIILAGPGGGLQQLD
EFFTQRLSLPTTQIDPIGALSLEVDAEKYPQVERSGLAIVLGLGMREV

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=312833 D1367_RS02210 WP_118171041.1 465680..466786(-) (pilM) [Nostoc sphaeroides strain Kutzing En]
GTGGTAAAAAGCTTCAAAAGTCTGTTTAGCAAATCAAAAAAAGGGGTCGGCATTGAACTTGCTCCCGAACGGGTGAATGT
AGTTCAGCTACGCAAGCAGGGTCAAGGCTTGAAGCTAGAAACCTTTACATCGGTAGCAGTTCCAGAAGGCGTAGTTACCG
ATGGTCAAATCACCGACCCCGCAGCAATGGCGCAATTAATCCAACAGGCGCTAACTGAGAGCAAAATCAAAACTTCTCGC
GTTGCCACTGGTGTACCAGGGCGAGATTCCATCGTTCGGATTATACCAGTGCCAGCAGAGTTAGATGATAAAGAACTGCG
AGAAATGGTTCTCAACCATGAAGCAGGTTTATATTTACCCTATCCTCGTGAAGAGGCTGATGTAGATTATCAGAAACTTG
GGTACAAGATGGATGAGGATGGCATAGAAAAGGTAGAGGTGCTTTTGGTTGCCACTCGTAAGGAAATTACTGATACCTAT
ATAAGTACCTTCGAGCAGGCAGGATTACAAATCGATATTTTAGAAATTAACAGTTTTGCTCTGATTCGGACTATTCGTGA
GCAATTGCGACAATTTGGGCCGCAAGAAGCAGCAGTGCTAGTTGATATAGAGTTCGACAGTACAGAAATCGCCATCATCG
TTAACGGAGTGCCGCAATTTTCGCGCACAGTCCCAATCGGGACTTATCAAATGCAAATGGCCTTAGCAAGGGCAATGAGC
TTACCCACATCACGAGATATGGAACTGTTACACGGAATGGTCATTCCCCCAACTTCTCTAGACGGCGGGAAAACTGGGGT
TACTGAAATCAATCCTGGTATGGCAGCCATATTGAGAGTATTGGGAGAACTAACGGATGAACTGCGCCGTTCCATCGATT
TTTACCTAAATCAAACTGAAAATATGGAGGTAGCGCAGATTATATTAGCTGGGCCAGGAGGTGGACTCCAACAGCTAGAT
GAATTCTTTACCCAACGATTGAGTTTGCCAACTACCCAAATAGATCCAATCGGCGCTTTGTCGTTGGAAGTTGACGCTGA
GAAATATCCACAGGTGGAACGCTCTGGCTTGGCGATCGTACTTGGTCTAGGAATGCGGGAGGTGTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-238)

Type IV pilus assembly protein PilM; (PilM_2)

(283-367)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

66.213

99.728

0.66

Multiple sequence alignment