Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   D0853_RS01840 Genome accession   NZ_CP031781
Coordinates   351598..352614 (+) Length   338 a.a.
NCBI ID   WP_005480475.1    Uniprot ID   A0A0L7V0I6
Organism   Vibrio parahaemolyticus strain VPD14     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 346598..357614
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0853_RS01825 (D0853_01830) - 347022..347750 (-) 729 WP_005465158.1 redoxin family protein -
  D0853_RS01830 (D0853_01835) oxyR 347921..348829 (+) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  D0853_RS01835 (D0853_01840) - 348885..351455 (-) 2571 WP_005480476.1 PBP1A family penicillin-binding protein -
  D0853_RS01840 (D0853_01845) pilM 351598..352614 (+) 1017 WP_005480475.1 type IV pilus assembly protein PilM Machinery gene
  D0853_RS01845 (D0853_01850) pilN 352598..353176 (+) 579 WP_005480484.1 PilN domain-containing protein Machinery gene
  D0853_RS01850 (D0853_01855) pilO 353169..353762 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  D0853_RS01855 (D0853_01860) pilP 353752..354267 (+) 516 WP_005480477.1 pilus assembly protein PilP Machinery gene
  D0853_RS01860 (D0853_01865) pilQ 354299..356041 (+) 1743 WP_024699327.1 type IV pilus secretin PilQ family protein Machinery gene
  D0853_RS01865 (D0853_01870) aroK 356230..356748 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37776.31 Da        Isoelectric Point: 6.8323

>NTDB_id=311221 D0853_RS01840 WP_005480475.1 351598..352614(+) (pilM) [Vibrio parahaemolyticus strain VPD14]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=311221 D0853_RS01840 WP_005480475.1 351598..352614(+) (pilM) [Vibrio parahaemolyticus strain VPD14]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAATTGAGTTTGGATTTCGTACGCCTTCGTGCGGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAAGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATCTCAATGGGGACGTCACAAGAAGACTTACTTTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAACGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTGTCGCATCAGTTGGCATTGGAGTGTGAGTTGTTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGCCGAGCGGCCGATTGGCAACATTTTTCGACGGCTGCA
GGGCTGGCGGTGCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0L7V0I6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.911

100

0.849
  pilM Vibrio cholerae strain A1552

48.368

99.704

0.482

Multiple sequence alignment