Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   CKAES1M_RS04150 Genome accession   NZ_CP037925
Coordinates   864687..865112 (-) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain AES1M     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 859687..870112
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKAES1M_RS04130 (CKAES1M_00813) ileS 859844..862675 (+) 2832 WP_003094730.1 isoleucine--tRNA ligase -
  CKAES1M_RS04135 (CKAES1M_00814) lspA 862668..863177 (+) 510 WP_003094728.1 signal peptidase II -
  CKAES1M_RS04140 (CKAES1M_00815) fkpB 863170..863610 (+) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  CKAES1M_RS04145 (CKAES1M_00816) ispH 863696..864640 (+) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  CKAES1M_RS04150 (CKAES1M_00817) comF 864687..865112 (-) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  CKAES1M_RS04155 (CKAES1M_00818) pilY2 865109..865456 (-) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  CKAES1M_RS04160 (CKAES1M_00819) pilY1 865458..868949 (-) 3492 WP_003123397.1 type 4a pilus biogenesis protein PilY1 -
  CKAES1M_RS04165 (CKAES1M_00820) pilX 868961..869548 (-) 588 WP_003112826.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=308738 CKAES1M_RS04150 WP_003094721.1 864687..865112(-) (comF) [Pseudomonas aeruginosa strain AES1M]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=308738 CKAES1M_RS04150 WP_003094721.1 864687..865112(-) (comF) [Pseudomonas aeruginosa strain AES1M]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGGCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383

Multiple sequence alignment