Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   E1J55_RS01445 Genome accession   NZ_CP037903
Coordinates   313191..313955 (-) Length   254 a.a.
NCBI ID   WP_089601759.1    Uniprot ID   -
Organism   Escherichia coli strain LHM10-1     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 308191..318955
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E1J55_RS01420 yhhJ 310034..311158 (+) 1125 WP_001216257.1 ABC transporter permease -
  E1J55_RS01425 - 311231..311505 (+) 275 Protein_285 type II toxin-antitoxin system HicA family toxin -
  E1J55_RS01430 - 311502..311861 (+) 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin -
  E1J55_RS01435 nikR 311981..312382 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  E1J55_RS01440 nikE 312388..313194 (-) 807 WP_089601760.1 nickel import ATP-binding protein NikE -
  E1J55_RS01445 amiE 313191..313955 (-) 765 WP_089601759.1 nickel import ATP-binding protein NikD Regulator
  E1J55_RS01450 nikC 313955..314788 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  E1J55_RS01455 nikB 314785..315729 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  E1J55_RS01460 nikA 315729..317303 (-) 1575 WP_089601758.1 nickel ABC transporter substrate-binding protein -
  E1J55_RS01465 acpT 317414..318001 (-) 588 WP_000285783.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26863.44 Da        Isoelectric Point: 6.5992

>NTDB_id=308568 E1J55_RS01445 WP_089601759.1 313191..313955(-) (amiE) [Escherichia coli strain LHM10-1]
MPQQIELRNIALQAAQPLVHSVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=308568 E1J55_RS01445 WP_089601759.1 313191..313955(-) (amiE) [Escherichia coli strain LHM10-1]
ATGCCGCAACAGATTGAACTACGTAATATCGCTCTACAGGCCGCGCAGCCGCTGGTGCACAGTGTATCGTTAACCCTGCA
ACGCGGGCGTGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTCTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCACGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.2

98.425

0.406
  amiE Streptococcus thermophilus LMD-9

41.2

98.425

0.406
  amiE Streptococcus salivarius strain HSISS4

40

98.425

0.394

Multiple sequence alignment