Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   EW646_RS20390 Genome accession   NZ_CP035770
Coordinates   4089065..4089532 (+) Length   155 a.a.
NCBI ID   WP_000381746.1    Uniprot ID   Q8X892
Organism   Escherichia coli O145 strain RM8988-C1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4084065..4094532
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW646_RS20345 (EW646_20865) rplP 4084175..4084585 (-) 411 WP_000941212.1 50S ribosomal protein L16 -
  EW646_RS20350 (EW646_20870) rpsC 4084598..4085299 (-) 702 WP_000529945.1 30S ribosomal protein S3 -
  EW646_RS20355 (EW646_20875) rplV 4085317..4085649 (-) 333 WP_000447529.1 50S ribosomal protein L22 -
  EW646_RS20360 (EW646_20880) rpsS 4085664..4085942 (-) 279 WP_001138117.1 30S ribosomal protein S19 -
  EW646_RS20365 (EW646_20885) rplB 4085959..4086780 (-) 822 WP_000301864.1 50S ribosomal protein L2 -
  EW646_RS20370 (EW646_20890) rplW 4086798..4087100 (-) 303 WP_000617544.1 50S ribosomal protein L23 -
  EW646_RS20375 (EW646_20895) rplD 4087097..4087702 (-) 606 WP_000424395.1 50S ribosomal protein L4 -
  EW646_RS20380 (EW646_20900) rplC 4087713..4088342 (-) 630 WP_000579833.1 50S ribosomal protein L3 -
  EW646_RS20385 (EW646_20905) rpsJ 4088375..4088686 (-) 312 WP_001181004.1 30S ribosomal protein S10 -
  EW646_RS20390 (EW646_20915) pilD 4089065..4089532 (+) 468 WP_000381746.1 A24 family peptidase Machinery gene
  EW646_RS20395 (EW646_20920) bfr 4089529..4090005 (-) 477 WP_000675515.1 bacterioferritin -
  EW646_RS20400 (EW646_20925) bfd 4090078..4090272 (-) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  EW646_RS20405 (EW646_20930) tuf 4090455..4091639 (-) 1185 WP_000031783.1 elongation factor Tu -
  EW646_RS20410 (EW646_20935) fusA 4091710..4093824 (-) 2115 WP_000124700.1 elongation factor G -
  EW646_RS20415 (EW646_20940) rpsG 4093921..4094391 (-) 471 WP_001138043.1 30S ribosomal protein S7 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 17099.29 Da        Isoelectric Point: 8.1343

>NTDB_id=304833 EW646_RS20390 WP_000381746.1 4089065..4089532(+) (pilD) [Escherichia coli O145 strain RM8988-C1]
MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFYQVCHPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHSWAFLPRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=304833 EW646_RS20390 WP_000381746.1 4089065..4089532(+) (pilD) [Escherichia coli O145 strain RM8988-C1]
ATGGACACTACCCTTCCCTTTCTAATTTTGTACGCTTGTTTATCAGCCCTACTTTTTTTCTGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACGTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTATCAAGTTTGTCATCCTGATGGTTTAGCCG
ATGCGTTATGGGGGGCAATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGCCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGCGCCTGGCATTCATGGGCTTTCTTACCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTATTGGTCTGCTCATGAGAGGAAAAGAATCATTAAAAAACCCGCTGC
CTTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTATAGGCTGGGATAGTTTGCTGATAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(11-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8X892

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

43.478

89.032

0.387

Multiple sequence alignment