Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   EWS90_RS25850 Genome accession   NZ_CP035739
Coordinates   5350946..5351371 (-) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain 1334/14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5345946..5356371
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EWS90_RS25830 (EWS90_25830) ileS 5346103..5348934 (+) 2832 WP_003102617.1 isoleucine--tRNA ligase -
  EWS90_RS25835 (EWS90_25835) lspA 5348927..5349436 (+) 510 WP_003102615.1 signal peptidase II -
  EWS90_RS25840 (EWS90_25840) fkpB 5349429..5349869 (+) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  EWS90_RS25845 (EWS90_25845) ispH 5349955..5350899 (+) 945 WP_003112824.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  EWS90_RS25850 (EWS90_25850) comF 5350946..5351371 (-) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  EWS90_RS25855 (EWS90_25855) pilY2 5351368..5351715 (-) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  EWS90_RS25860 (EWS90_25860) pilY1 5351717..5355208 (-) 3492 WP_003123397.1 type 4a pilus biogenesis protein PilY1 -
  EWS90_RS25865 (EWS90_25865) pilX 5355220..5355807 (-) 588 WP_034037187.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=304530 EWS90_RS25850 WP_003094721.1 5350946..5351371(-) (comF) [Pseudomonas aeruginosa strain 1334/14]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=304530 EWS90_RS25850 WP_003094721.1 5350946..5351371(-) (comF) [Pseudomonas aeruginosa strain 1334/14]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383