Detailed information NTG0294069

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: EQJ84_RS08430
• Genome accession: NZ_CP035191
• Coordinates: 1615644..1618061 (+)
• Length: 805 a.a.
• NCBI ID: WP_041850844.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain SRCM104011
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1610644..1623061

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EQJ84_RS08405 (EQJ84_08405)	endC	1612296..1613171 (+)	876	WP_009967215.1	YicC/YloC family endoribonuclease	-
EQJ84_RS08410 (EQJ84_08410)	remA	1613248..1613517 (+)	270	WP_003154355.1	extracellular matrix/biofilm regulator RemA	-
EQJ84_RS08415 (EQJ84_08415)	gmk	1613525..1614139 (+)	615	WP_003232083.1	guanylate kinase	-
EQJ84_RS08420 (EQJ84_08420)	rpoZ	1614143..1614346 (+)	204	WP_003221520.1	DNA-directed RNA polymerase subunit omega	-
EQJ84_RS08425 (EQJ84_08425)	coaBC	1614427..1615647 (+)	1221	WP_041850845.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
EQJ84_RS08430 (EQJ84_08430)	priA	1615644..1618061 (+)	2418	WP_041850844.1	primosomal protein N'	Machinery gene
EQJ84_RS08435 (EQJ84_08435)	def	1618088..1618570 (+)	483	WP_015383630.1	peptide deformylase	-
EQJ84_RS08440 (EQJ84_08440)	fmt	1618575..1619528 (+)	954	WP_128441623.1	methionyl-tRNA formyltransferase	-
EQJ84_RS08445 (EQJ84_08445)	rsmB	1619515..1620858 (+)	1344	WP_021479311.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
EQJ84_RS08450 (EQJ84_08450)	rlmN	1620862..1621953 (+)	1092	WP_021479310.1	23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN	-
EQJ84_RS08455 (EQJ84_08455)	prpC	1621960..1622724 (+)	765	WP_014479762.1	protein-serine/threonine phosphatase PrpC	-

Sequence

Protein

• Length: 805 a.a.
• Molecular weight: 91381.43 Da
• Isoelectric Point: 8.4340

>NTDB_id=294069 EQJ84_RS08430 WP_041850844.1 1615644..1618061(+) (priA) [Bacillus subtilis strain SRCM104011]
MNFAEVIVDVSTKNIDRPFDYKIPDHLKGMIKTGMRVIVPFGPRKIQGFVTAVKEASDLSGKSVKEVEDLLDLTPVLTEE
LMDLSSWLSDKTLSFKITALQAMLPAALKAKYEKELKIVHGADLPPQVERLFSETKTLLYSDIPDHDTLKLIQRHVQKGV
IDVTYKVAQKTNKKMVRHIQANASKEELAKQAEGLSRQAAKQQAILHFLISEPEGVKIPAAELCKRTDTSSATIKTLIQK
GLLKESYEEVYRDPYQDKMFKKTEPLPLTDEQRAAFEPIRETLDSDEHKVFLLHGVTGSGKTEIYLQSIEKVLAKGKEAI
VLVPEISLTPQMVNRFKGRFGSQVAVMHSGLSTGEKYDEWRKIHRKEVRLVVGARSAIFAPFENLGMIIIDEEHESSYKQ
EEMPRYHAKEVAIKRAEHHSCPVVLGSATPTLESYARAQKGVYELLSLKHRVNHRVMPEVSLVDMREELRNGNRSMFSVE
LMEKLEETIAKGEQAVLFLNKRGYSSFVMCRDCGYVPQCPHCDISMTYHRYGQRLKCHYCGHEEPVPHTCPECASEHIRF
FGTGTQRVEEELTKVLPSARVIRMDVDTTSRKGAHEKLLSAFGEGKADILLGTQMIAKGLDFPNVTLVGVLSADTTLHIP
DFRSAEKTFQLLTQVSGRAGRHEKPGHVIIQTYTPSHYSIQLTKTHDYETFYQHEMAHRREQSYPPYYYLALVTVSHEEV
AKAAVTAEKIAHFLKANCGADTKILGPSASPIARIKDRYRYQCVIKYKQETQLSALLKKILEHYKREIEQKHVMISIDMN
PYMMM

Nucleotide

• Length: 2418 bp

>NTDB_id=294069 EQJ84_RS08430 WP_041850844.1 1615644..1618061(+) (priA) [Bacillus subtilis strain SRCM104011]
ATGAATTTTGCAGAAGTCATCGTTGATGTCAGCACCAAAAATATAGACAGGCCTTTTGATTATAAAATCCCAGACCATCT
GAAGGGCATGATCAAAACGGGGATGCGGGTCATTGTTCCGTTTGGCCCCCGTAAGATTCAAGGGTTTGTGACAGCAGTCA
AAGAAGCATCTGACCTATCGGGAAAATCTGTCAAGGAAGTAGAGGATTTATTAGATCTTACACCTGTTCTGACAGAGGAG
CTTATGGATCTGTCATCATGGCTGTCAGACAAAACGCTGTCTTTTAAAATAACGGCGCTTCAAGCAATGCTGCCTGCCGC
CTTAAAGGCGAAATATGAAAAAGAATTAAAAATTGTACACGGCGCCGATCTTCCGCCGCAAGTGGAACGGCTGTTTTCTG
AAACAAAGACGCTGCTGTATTCAGATATACCCGATCATGATACGCTGAAACTGATTCAAAGGCATGTTCAAAAAGGGGTC
ATTGACGTGACGTATAAAGTCGCGCAAAAAACCAATAAAAAAATGGTCAGGCATATTCAGGCAAATGCGAGTAAAGAAGA
GCTTGCTAAGCAGGCTGAGGGATTGTCCCGACAAGCAGCAAAACAGCAGGCGATTCTTCACTTTCTCATCTCTGAGCCGG
AAGGTGTGAAAATTCCTGCAGCGGAATTGTGCAAAAGAACGGACACGAGCTCTGCAACCATCAAAACCCTTATCCAAAAA
GGGCTTTTGAAGGAAAGCTATGAAGAGGTTTACAGAGATCCCTATCAGGACAAAATGTTCAAAAAAACAGAGCCCCTGCC
GCTGACAGACGAACAGAGGGCTGCCTTTGAGCCCATACGCGAGACATTGGACAGCGATGAGCATAAAGTGTTCCTCCTTC
ACGGCGTGACAGGAAGCGGAAAAACGGAAATTTATCTGCAATCAATTGAAAAAGTACTAGCGAAAGGAAAAGAAGCCATC
GTCCTCGTACCGGAAATATCGCTGACGCCGCAAATGGTCAACCGCTTCAAGGGCCGCTTCGGCTCCCAGGTGGCAGTCAT
GCACAGCGGTTTATCCACAGGAGAAAAATATGACGAGTGGCGTAAAATCCACCGGAAGGAAGTACGGCTTGTCGTCGGTG
CCAGATCTGCGATTTTTGCTCCGTTTGAAAATCTCGGAATGATAATCATCGATGAAGAGCACGAGTCTTCCTATAAACAG
GAAGAAATGCCGCGCTATCATGCGAAAGAGGTGGCAATCAAGCGGGCTGAGCATCACAGCTGCCCGGTTGTATTAGGGAG
TGCTACGCCGACATTAGAGTCATATGCAAGAGCGCAAAAGGGCGTATACGAGCTTCTTTCACTGAAGCACCGTGTTAACC
ACCGGGTGATGCCTGAGGTTTCGCTTGTCGATATGAGAGAGGAGCTTAGAAACGGCAATCGTTCGATGTTTTCAGTTGAA
TTGATGGAAAAGCTTGAAGAAACGATTGCCAAGGGTGAGCAGGCGGTGCTGTTTTTAAATAAGAGGGGCTATTCTTCCTT
TGTGATGTGCAGGGATTGCGGCTATGTCCCGCAATGCCCGCACTGCGACATTTCCATGACGTATCATCGTTACGGCCAAA
GGCTGAAATGCCATTATTGCGGACATGAAGAGCCTGTTCCGCACACTTGTCCTGAATGCGCAAGCGAGCATATCCGCTTT
TTTGGAACGGGAACACAGCGAGTGGAGGAAGAGCTTACAAAAGTGCTGCCAAGTGCGAGAGTGATTCGGATGGATGTTGA
CACGACATCACGGAAAGGCGCCCATGAAAAATTACTGTCAGCTTTCGGAGAAGGAAAAGCGGATATTCTTCTCGGAACGC
AAATGATCGCGAAAGGGCTTGATTTTCCGAATGTCACGCTTGTCGGAGTGTTAAGTGCTGATACAACACTTCATATTCCT
GATTTCAGATCGGCTGAAAAAACATTTCAGCTGTTAACGCAAGTCAGCGGCCGGGCAGGCAGGCATGAAAAGCCCGGCCA
TGTCATCATTCAAACGTATACACCGTCTCATTACAGTATCCAGCTGACGAAAACACACGATTATGAAACGTTCTATCAGC
ATGAAATGGCGCACAGGAGAGAGCAATCCTATCCGCCATACTACTATTTGGCTCTTGTGACTGTTTCTCATGAGGAAGTG
GCGAAAGCAGCGGTTACAGCTGAAAAGATCGCTCATTTTTTGAAAGCAAACTGCGGAGCTGACACGAAAATTCTCGGACC
AAGCGCGTCGCCGATAGCCAGGATCAAAGATAGATATCGCTATCAATGCGTGATAAAATACAAACAGGAAACCCAGTTAT
CCGCTTTATTGAAGAAGATACTGGAACATTATAAACGCGAAATTGAACAAAAACATGTAATGATTTCAATTGATATGAAT
CCTTATATGATGATGTAA

Domains

Predicted by InterProScan.

• Primosomal protein N C-terminal domain (PriA_C) (706-801)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-105)

• Type III restriction enzyme, res subunit (ResIII) (267-431)

• Helicase conserved C-terminal domain (Helicase_C) (565-662)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (519-545)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	99.379	100	0.994