Detailed information NTG0280104

Bioinformatically predicted

Overview

• Name: pilR
• Type: Regulator
• Locus tag: C4K04_RS27525
• Genome accession: NZ_CP027753
• Coordinates: 6005007..6006335 (+)
• Length: 442 a.a.
• NCBI ID: WP_124322376.1
• Uniprot ID: A0A3G7TVU8
• Organism: Pseudomonas chlororaphis strain B25
• Function: regulate pilin expression (predicted from homology) Competence regulation

Genomic Context

Location: 6000007..6011335

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C4K04_RS27505 (C4K04_5591)	pgeF	6001496..6002221 (-)	726	WP_124322375.1	peptidoglycan editing factor PgeF	-
C4K04_RS27510 (C4K04_5592)	rluD	6002218..6003180 (-)	963	WP_007924719.1	23S rRNA pseudouridine(1911/1915/1917) synthase RluD	-
C4K04_RS27515 (C4K04_5593)	-	6003333..6004355 (+)	1023	WP_007924720.1	outer membrane protein assembly factor BamD	-
C4K04_RS27520 (C4K04_5594)	-	6004489..6004722 (+)	234	WP_007924721.1	PP0621 family protein	-
C4K04_RS27525 (C4K04_5595)	pilR	6005007..6006335 (+)	1329	WP_124322376.1	sigma-54 dependent transcriptional regulator	Regulator
C4K04_RS27530 (C4K04_5596)	thiO	6006332..6007432 (-)	1101	WP_124322377.1	glycine oxidase ThiO	-
C4K04_RS27535 (C4K04_5597)	-	6007487..6007888 (-)	402	WP_007924724.1	type IV pilin protein	-
C4K04_RS27540 (C4K04_5598)	-	6007902..6010973 (-)	3072	WP_124322378.1	PilC/PilY family type IV pilus protein	-

Sequence

Protein

• Length: 442 a.a.
• Molecular weight: 48772.16 Da
• Isoelectric Point: 8.6757

>NTDB_id=280104 C4K04_RS27525 WP_124322376.1 6005007..6006335(+) (pilR) [Pseudomonas chlororaphis strain B25]
MSPRQKVLIVDDDPGIREYLEISLGRMQRHTRSARTLGEARHWLAREGFDLCLSDLHLPDGTGLELLEHIQRHRLRMQLA
ILSTCPCQETAIHALKAGAIDFLSKPVALARLRELLNGTLPGSTPANPATGPLLGDSTAMHKLRVQIGKLAQSQASVYIS
GESGSGKERVARAIHDQGPRASRGFVPVNCGAIPAELMESEFFGHRKGSFSGAIEDKPGLFQAAHGGTLFLDEVADLPLA
MQVKLLRALQEKAVRCVGGQQEVQVDVRILCATHKTLQAEVHAGRFRQDLYYRLNVIELAVPPLRARKADIEPLATAILE
RLAQGNGQQLARLQPGALLALQGYDFPGNVRELENLLERAHTLCENRQIEARDLCLTPNSAHPAIAPGPAQVIDLEGYLQ
EVERRLILQALEHTRWNRTAAAQRLSLSFRSMRYRLKKLGLD

Nucleotide

• Length: 1329 bp

>NTDB_id=280104 C4K04_RS27525 WP_124322376.1 6005007..6006335(+) (pilR) [Pseudomonas chlororaphis strain B25]
ATGAGCCCACGGCAAAAAGTCCTGATCGTCGACGACGATCCAGGCATTCGCGAATATCTGGAAATCAGCCTCGGCCGGAT
GCAGCGTCATACCCGCAGCGCCCGCACCCTGGGCGAAGCGCGTCACTGGCTGGCCCGGGAAGGTTTCGACCTGTGCCTGA
GCGACCTGCATCTGCCCGACGGCACGGGGCTGGAATTACTCGAGCATATCCAGCGCCACCGGCTGCGAATGCAGTTGGCC
ATACTCAGCACCTGCCCCTGCCAGGAAACAGCGATCCACGCCCTGAAGGCCGGCGCCATCGACTTCCTGAGCAAACCCGT
GGCCCTGGCGCGTCTGCGTGAGCTGCTGAACGGCACCCTGCCCGGATCGACACCAGCGAACCCAGCTACCGGCCCACTGC
TCGGCGATTCGACCGCCATGCACAAACTGCGCGTGCAGATCGGCAAGCTGGCCCAGAGCCAGGCCTCGGTCTATATCAGC
GGAGAGTCCGGCAGCGGCAAGGAACGGGTCGCCCGCGCGATCCACGACCAGGGACCACGGGCCAGCCGGGGCTTCGTGCC
GGTCAACTGCGGGGCCATCCCGGCGGAGCTGATGGAGAGCGAGTTTTTCGGCCATCGCAAAGGCAGCTTCAGCGGCGCTA
TCGAAGACAAGCCCGGCCTGTTCCAGGCAGCGCACGGCGGCACGCTGTTTCTCGATGAGGTGGCCGATCTGCCGCTGGCG
ATGCAGGTCAAATTGCTCCGCGCACTGCAGGAGAAGGCGGTACGTTGCGTCGGTGGACAGCAGGAGGTCCAGGTCGATGT
GCGCATTCTCTGCGCCACCCACAAGACGCTGCAGGCGGAAGTACATGCCGGGCGCTTTCGCCAGGATCTCTACTACCGTC
TGAACGTGATCGAGCTGGCGGTGCCACCGCTAAGGGCGCGCAAGGCTGATATCGAACCCTTGGCCACCGCCATCCTCGAG
CGCCTGGCGCAGGGCAACGGCCAACAGTTGGCGCGGCTCCAGCCAGGCGCGCTGCTGGCACTCCAGGGCTACGACTTTCC
CGGCAACGTGCGCGAGCTGGAGAACCTGCTCGAACGGGCGCACACCCTGTGCGAGAACCGGCAGATCGAAGCACGCGATC
TATGCCTGACGCCCAATAGCGCCCACCCGGCCATCGCGCCCGGCCCGGCACAGGTGATCGACCTTGAAGGTTATCTGCAG
GAAGTCGAGCGCCGGCTGATCCTCCAGGCCCTGGAGCACACCCGCTGGAACCGCACGGCGGCGGCCCAGCGCTTGAGCCT
GTCGTTTCGTTCGATGCGCTACAGGCTGAAGAAACTCGGCCTGGATTAA

Domains

Predicted by InterproScan.

• Sigma-54 interaction domain (Sigma54_activat) (134-299)

• Response regulator receiver domain (Response_reg) (7-116)

• Bacterial regulatory protein, Fis family (HTH_8) (399-439)

3D structure

• AlphaFold DB: A0A3G7TVU8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilR	Pseudomonas aeruginosa PAK	66.667	100	0.679
pilR	Acinetobacter baumannii strain A118	44.009	100	0.457