Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiF   Type   Regulator
Locus tag   SPYM_RS01345 Genome accession   NC_009332
Coordinates   256051..256974 (+) Length   307 a.a.
NCBI ID   WP_002986000.1    Uniprot ID   -
Organism   Streptococcus pyogenes str. Manfredo     
Function   internalize XIP (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IScluster/Tn 257536..258611 256051..256974 flank 562


Gene organization within MGE regions


Location: 256051..258611
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPYM_RS01345 (SpyM50231) amiF 256051..256974 (+) 924 WP_002986000.1 ATP-binding cassette domain-containing protein Regulator
  SPYM_RS10140 - 257012..257095 (-) 84 Protein_224 IS3 family transposase -
  SPYM_RS09915 - 257122..257301 (-) 180 WP_111695866.1 transposase -
  SPYM_RS09960 - 257599..258611 (-) 1013 Protein_226 IS3 family transposase -

Sequence

Protein


Download         Length: 307 a.a.        Molecular weight: 34715.90 Da        Isoelectric Point: 6.5113

>NTDB_id=27847 SPYM_RS01345 WP_002986000.1 256051..256974(+) (amiF) [Streptococcus pyogenes str. Manfredo]
MSEKLVEVKDLEISFGEGKKKFVAVKNANFFIKKGETFSLVGESGSGKTTIGRAIIGLNDTSSGQILYDGKVINGRKSKS
EANELIRKIQMIFQDPAASLNERATVDYIISEGLYNFNLFKTEEERKEKIKNMMAEVGLLSEHLTRYPHEFSGGQRQRIG
IARALVMNPEFVIADEPISALDVSVRAQVLNLLKRMQAEKGLTYLFIAHDLSVVRFISDRIAVIHKGVIVEVAETEELFN
NPIHPYTQSLLSAVPIPDPILERQKELVVYHPDQHDYTLDKPSMVEIKPNHFVWANQAEIEKYQKEL

Nucleotide


Download         Length: 924 bp        

>NTDB_id=27847 SPYM_RS01345 WP_002986000.1 256051..256974(+) (amiF) [Streptococcus pyogenes str. Manfredo]
ATGTCTGAGAAATTAGTCGAAGTAAAAGACCTAGAAATTTCCTTCGGAGAAGGAAAGAAAAAATTTGTTGCAGTTAAAAA
TGCTAATTTCTTTATTAAAAAAGGAGAAACCTTTTCTTTAGTTGGAGAATCTGGGAGTGGTAAAACAACAATTGGTCGTG
CTATTATTGGTTTGAACGATACTAGTTCAGGTCAAATTTTATACGATGGGAAAGTAATTAATGGCAGAAAATCAAAATCA
GAAGCCAATGAGCTCATTCGTAAAATTCAAATGATTTTCCAAGATCCCGCTGCTAGTTTGAATGAACGAGCAACCGTTGA
CTACATCATTTCAGAAGGTCTTTATAACTTTAATCTGTTTAAAACAGAGGAAGAACGTAAAGAAAAAATTAAGAACATGA
TGGCCGAAGTTGGTTTGCTATCAGAGCATTTGACGCGCTACCCACATGAATTTTCAGGAGGTCAACGTCAGCGGATCGGT
ATCGCTAGAGCCCTAGTAATGAACCCTGAATTCGTTATTGCTGATGAACCGATTTCAGCTTTGGACGTTTCCGTTCGCGC
ACAGGTTTTAAACCTTCTCAAACGGATGCAAGCCGAAAAAGGTTTGACTTATCTCTTCATTGCCCATGATCTTTCAGTCG
TTCGCTTTATTTCAGATCGTATTGCGGTTATCCATAAAGGGGTTATTGTAGAAGTTGCAGAAACAGAAGAACTGTTTAAT
AACCCAATTCATCCCTACACCCAATCCTTGTTATCAGCCGTGCCTATCCCAGATCCAATTTTAGAACGTCAAAAAGAACT
TGTTGTCTATCATCCAGACCAGCATGATTATACATTAGATAAGCCATCGATGGTTGAAATCAAACCAAATCACTTTGTTT
GGGCAAACCAAGCAGAAATTGAAAAATATCAAAAAGAATTGTAA

Domains

Predicted by InterproScan.

ABC transporter (ABC_tran)

(25-178)

Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY)

(230-262)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiF Streptococcus thermophilus LMG 18311

82.353

99.674

0.821
  amiF Streptococcus thermophilus LMD-9

82.026

99.674

0.818
  amiF Streptococcus salivarius strain HSISS4

82.026

99.674

0.818

Multiple sequence alignment