Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   D0T88_RS21745 Genome accession   NZ_CP031706
Coordinates   4497721..4498485 (+) Length   254 a.a.
NCBI ID   WP_001136236.1    Uniprot ID   A7ZT17
Organism   Escherichia coli strain R1     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 4492721..4503485
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0T88_RS21725 (D0T88_23345) acpT 4493675..4494262 (+) 588 WP_000285784.1 4'-phosphopantetheinyl transferase AcpT -
  D0T88_RS21730 (D0T88_23350) nikA 4494373..4495947 (+) 1575 WP_063113579.1 nickel ABC transporter substrate-binding protein -
  D0T88_RS21735 (D0T88_23355) nikB 4495947..4496891 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  D0T88_RS21740 (D0T88_23360) nikC 4496888..4497721 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  D0T88_RS21745 (D0T88_23365) amiE 4497721..4498485 (+) 765 WP_001136236.1 nickel import ATP-binding protein NikD Regulator
  D0T88_RS21750 (D0T88_23370) nikE 4498482..4499288 (+) 807 WP_000173666.1 nickel import ATP-binding protein NikE -
  D0T88_RS21755 (D0T88_23375) nikR 4499294..4499695 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  D0T88_RS21760 (D0T88_23380) - 4499894..4500640 (+) 747 WP_000839066.1 GntR family transcriptional regulator -
  D0T88_RS21765 (D0T88_23385) - 4500665..4501138 (+) 474 WP_001161553.1 PTS sugar transporter subunit IIA -
  D0T88_RS21770 (D0T88_23390) - 4501135..4501416 (+) 282 WP_000084022.1 PTS sugar transporter subunit IIB -
  D0T88_RS21775 (D0T88_23395) - 4501493..4502851 (+) 1359 WP_063113578.1 PTS galactitol transporter subunit IIC -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26833.42 Da        Isoelectric Point: 6.5992

>NTDB_id=270675 D0T88_RS21745 WP_001136236.1 4497721..4498485(+) (amiE) [Escherichia coli strain R1]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=270675 D0T88_RS21745 WP_001136236.1 4497721..4498485(+) (amiE) [Escherichia coli strain R1]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZT17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398

Multiple sequence alignment