Detailed information NTG0026646

Bioinformatically predicted

Overview

• Name: amiF
• Type: Regulator
• Locus tag: STER_RS01115
• Genome accession: NC_008532
• Coordinates: 193240..194178 (+)
• Length: 312 a.a.
• NCBI ID: WP_011680672.1
• Uniprot ID: A0A0E2Q3C4
• Organism: Streptococcus thermophilus LMD-9
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 188240..199178

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
STER_RS12075	-	189904..190110 (+)	207	WP_011225370.1	hypothetical protein	-
STER_RS01085 (STER_0223)	-	190195..190939 (+)	745	Protein_160	peptide ABC transporter substrate-binding protein	-
STER_RS01090 (STER_0224)	-	190920..191138 (+)	219	Protein_161	ABC transporter permease subunit	-
STER_RS01095 (STER_0225)	-	191149..192182 (+)	1034	Protein_162	ABC transporter permease	-
STER_RS09980 (STER_0226)	-	192196..193238 (+)	1043	Protein_163	ABC transporter ATP-binding protein	-
STER_RS01115 (STER_0227)	amiF	193240..194178 (+)	939	WP_011680672.1	ABC transporter ATP-binding protein	Regulator
STER_RS11530 (STER_0229)	-	194400..194588 (+)	189	WP_011225379.1	hypothetical protein	-
STER_RS01125 (STER_0230)	hslO	194735..195601 (+)	867	WP_002949275.1	Hsp33 family molecular chaperone HslO	-
STER_RS01130 (STER_0231)	dusB	195594..196571 (+)	978	WP_011680674.1	tRNA dihydrouridine synthase DusB	-
STER_RS01135 (STER_0232)	-	196835..198067 (+)	1233	WP_041827633.1	peptidase	-
STER_RS01140 (STER_0233)	-	198173..198616 (+)	444	WP_011680676.1	zinc-dependent MarR family transcriptional regulator	-

Sequence

Protein

• Length: 312 a.a.
• Molecular weight: 35353.03 Da
• Isoelectric Point: 6.1917

>NTDB_id=26646 STER_RS01115 WP_011680672.1 193240..194178(+) (amiF) [Streptococcus thermophilus LMD-9]
MTQERKKLVELKNVSLTFNKGKSNEVEAIDDVSFDIYEGEIFGLVGESGSGKTTIGRFVLKLSDIDSGTVTFSGNEISSL
KGKDLHDFRKIDQMIFQDPQSSLNGRMKIRDIIAEGIDIHKLAKSKEEREAKVKELIDLVGLNEDHLSRYPHEFSGGQRQ
RIGIARALAVNHHFIVADEPISALDVSIQAQVVNLMQKLQHEQGLTYLFIAHDLSIVKYISDRIGAMHWGKLLEVGPADE
IYHNPIHPYTKSLLSAIPEPDPERERKRQHLIYDSSIENDGENRQMYEIRPGHFVYTTVAEAKNYKEEVKSN

Nucleotide

• Length: 939 bp

>NTDB_id=26646 STER_RS01115 WP_011680672.1 193240..194178(+) (amiF) [Streptococcus thermophilus LMD-9]
ATGACACAAGAACGTAAAAAGTTAGTAGAACTAAAAAATGTGTCTCTAACTTTTAATAAAGGAAAATCAAACGAGGTTGA
GGCTATTGATGACGTTAGCTTTGATATCTATGAGGGCGAAATTTTTGGACTAGTTGGAGAATCTGGATCAGGTAAAACAA
CCATTGGGCGATTTGTTTTAAAACTTTCTGATATTGATTCGGGTACTGTTACCTTTTCAGGTAATGAGATATCAAGTTTA
AAAGGGAAAGATCTTCATGATTTCCGTAAGATAGATCAGATGATCTTTCAAGATCCTCAATCTAGTCTTAATGGACGCAT
GAAAATCCGTGATATTATTGCTGAAGGCATTGATATTCATAAATTGGCTAAATCTAAGGAAGAGCGTGAAGCTAAGGTTA
AAGAACTCATTGATTTGGTAGGGTTAAATGAAGATCACCTATCAAGATATCCCCATGAGTTTTCTGGTGGTCAACGTCAA
AGGATTGGTATTGCGCGTGCTTTAGCGGTTAATCATCATTTTATTGTAGCAGACGAACCGATTTCTGCTTTGGATGTTTC
TATTCAAGCACAAGTGGTTAATTTGATGCAGAAGTTGCAACATGAACAGGGGTTAACTTATTTGTTTATTGCCCATGACC
TATCGATTGTTAAATATATATCCGATCGTATTGGTGCAATGCATTGGGGTAAACTTCTTGAAGTGGGACCAGCAGATGAG
ATCTATCATAACCCGATTCATCCTTATACAAAGAGTCTATTATCTGCAATTCCAGAACCAGATCCAGAAAGAGAGCGTAA
GCGTCAGCATTTGATTTACGATTCCTCTATTGAAAATGATGGGGAAAATAGACAAATGTATGAAATCCGACCAGGGCATT
TTGTTTATACTACAGTAGCAGAGGCTAAAAATTATAAAGAAGAAGTCAAGTCTAATTAA

Domains

Predicted by InterproScan.

• ABC transporter (ABC_tran) (30-181)

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-265)

3D structure

• AlphaFold DB: A0A0E2Q3C4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiF	Streptococcus thermophilus LMD-9	53.115	97.756	0.519
amiF	Streptococcus thermophilus LMG 18311	52.787	97.756	0.516
amiF	Streptococcus salivarius strain HSISS4	52.459	97.756	0.513