Detailed information NTG0266387

Bioinformatically predicted

Overview

• Name: endA
• Type: Machinery gene
• Locus tag: DY328_RS08475
• Genome accession: NZ_CP031556
• Coordinates: 1558501..1559286 (+)
• Length: 261 a.a.
• NCBI ID: WP_000825431.1
• Uniprot ID: Q8E0P2
• Organism: Streptococcus agalactiae strain Sag27
• Function: cleavage of dsDNA into ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1553501..1564286

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DY328_RS08445 (DY328_08445)	-	1554618..1555061 (+)	444	WP_001048166.1	hypothetical protein	-
DY328_RS08450 (DY328_08450)	-	1555146..1555412 (+)	267	WP_001865676.1	hypothetical protein	-
DY328_RS08455 (DY328_08455)	-	1555513..1555641 (+)	129	WP_000962057.1	hypothetical protein	-
DY328_RS08460 (DY328_08460)	-	1555650..1555970 (+)	321	WP_001865677.1	hypothetical protein	-
DY328_RS08465 (DY328_08465)	-	1556067..1556738 (+)	672	WP_000891268.1	ABC transporter ATP-binding protein	-
DY328_RS08470 (DY328_08470)	-	1556795..1558240 (-)	1446	WP_000750933.1	FAD/NAD(P)-binding protein	-
DY328_RS08475 (DY328_08475)	endA	1558501..1559286 (+)	786	WP_000825431.1	DNA/RNA non-specific endonuclease	Machinery gene
DY328_RS08480 (DY328_08480)	-	1559364..1560002 (-)	639	WP_000478529.1	VTT domain-containing protein	-
DY328_RS08485 (DY328_08485)	-	1560219..1560875 (+)	657	WP_000121094.1	ATP-binding cassette domain-containing protein	-
DY328_RS08490 (DY328_08490)	fetB	1560872..1561645 (+)	774	WP_000012390.1	iron export ABC transporter permease subunit FetB	-
DY328_RS08495 (DY328_08495)	-	1561809..1562627 (+)	819	WP_000601007.1	hypothetical protein	-
DY328_RS08500 (DY328_08500)	-	1562664..1563548 (-)	885	WP_000354840.1	LysR family transcriptional regulator	-
DY328_RS08505 (DY328_08505)	-	1563741..1564247 (+)	507	Protein_1556	PTS sugar transporter subunit IIC	-

Sequence

Protein

• Length: 261 a.a.
• Molecular weight: 29424.86 Da
• Isoelectric Point: 8.9989

>NTDB_id=266387 DY328_RS08475 WP_000825431.1 1558501..1559286(+) (endA) [Streptococcus agalactiae strain Sag27]
MKRLHKLFITVIATLGMLGVMTFGLPTQPQNVTPIVHADVNSSVDTSQEFQNNLKNAIGNLPFQYVNGIYELNNNQTNLN
ADVNVKAYVQNTIDNQQRLSTANAMLDRTIRQYQNRRDTTLPDANWKPLGWHQVATNDHYGHAVDKGHLIAYALAGNFKG
WDASVSNPQNVVTQTAHSNQSNQKINRGQNYYESLVRKAVDQNKRVRYRVTPLYRNDTDLVPFAMHLEAKSQDGTLEFNV
AIPNTQASYTMDYATGEITLN

Nucleotide

• Length: 786 bp

>NTDB_id=266387 DY328_RS08475 WP_000825431.1 1558501..1559286(+) (endA) [Streptococcus agalactiae strain Sag27]
ATGAAAAGATTACATAAACTGTTTATAACCGTAATTGCTACATTAGGTATGTTGGGGGTAATGACCTTTGGTCTTCCAAC
GCAGCCGCAAAACGTAACGCCGATAGTACATGCTGATGTCAATTCATCTGTTGATACGAGCCAGGAATTTCAAAATAATT
TAAAAAATGCTATTGGTAACCTACCATTTCAATATGTTAATGGTATTTATGAATTAAATAATAATCAGACAAATTTAAAT
GCTGATGTCAATGTTAAAGCGTATGTTCAAAATACAATTGACAATCAACAAAGACTATCAACTGCTAATGCAATGCTTGA
TAGAACCATTCGTCAATATCAAAATCGCAGAGATACCACTCTTCCCGATGCAAATTGGAAACCATTAGGTTGGCATCAAG
TAGCTACTAATGACCATTATGGACATGCAGTCGACAAGGGGCATTTAATTGCCTATGCTTTAGCTGGAAATTTCAAAGGT
TGGGATGCTTCCGTGTCAAATCCTCAAAATGTTGTCACACAAACAGCTCATTCCAACCAATCAAATCAAAAAATCAATCG
TGGACAAAATTATTATGAAAGCTTAGTTCGTAAGGCGGTTGACCAAAACAAACGTGTTCGTTACCGTGTAACTCCATTGT
ACCGTAATGATACTGATTTAGTTCCATTTGCAATGCACCTAGAAGCTAAATCACAAGATGGCACATTAGAATTTAATGTT
GCTATTCCAAACACACAAGCATCATACACTATGGATTATGCAACAGGAGAAATAACACTAAATTAA

Domains

Predicted by InterProScan.

• DNA/RNA non-specific endonuclease (Endonuclease_NS) (77-235)

3D structure

• AlphaFold DB: Q8E0P2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
endA	Streptococcus pneumoniae Rx1	51.515	75.862	0.391
endA	Streptococcus pneumoniae D39	51.515	75.862	0.391
endA	Streptococcus pneumoniae R6	51.515	75.862	0.391
endA	Streptococcus pneumoniae TIGR4	51.515	75.862	0.391