Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   CWI88_RS02425 Genome accession   NZ_CP025225
Coordinates   497421..498854 (-) Length   477 a.a.
NCBI ID   WP_006178789.1    Uniprot ID   -
Organism   Enterobacter cancerogenus strain CR-Eb1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 492421..503854
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWI88_RS02395 (CWI88_02395) elbB 493953..494606 (+) 654 WP_101736894.1 isoprenoid biosynthesis glyoxalase ElbB -
  CWI88_RS02400 (CWI88_02400) mtgA 494603..495328 (+) 726 WP_101736895.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  CWI88_RS02405 (CWI88_02405) npr 495368..495640 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  CWI88_RS02410 (CWI88_02410) rapZ 495637..496491 (-) 855 WP_006178792.1 RNase adapter RapZ -
  CWI88_RS02415 (CWI88_02415) ptsN 496537..497028 (-) 492 WP_006178791.1 PTS IIA-like nitrogen regulatory protein PtsN -
  CWI88_RS02420 (CWI88_02420) hpf 497111..497398 (-) 288 WP_006178790.1 ribosome hibernation promoting factor -
  CWI88_RS02425 (CWI88_02425) rpoN/rpoN1 497421..498854 (-) 1434 WP_006178789.1 RNA polymerase factor sigma-54 Machinery gene
  CWI88_RS02430 (CWI88_02430) lptB 498902..499627 (-) 726 WP_006178788.1 LPS export ABC transporter ATP-binding protein -
  CWI88_RS02435 (CWI88_02435) lptA 499634..500188 (-) 555 WP_101736896.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  CWI88_RS02440 (CWI88_02440) lptC 500157..500732 (-) 576 WP_006178786.1 LPS export ABC transporter periplasmic protein LptC -
  CWI88_RS02445 (CWI88_02445) kdsC 500729..501295 (-) 567 WP_101736897.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  CWI88_RS02450 (CWI88_02450) kdsD 501310..502296 (-) 987 WP_006178784.1 arabinose-5-phosphate isomerase KdsD -
  CWI88_RS02455 (CWI88_02455) - 502310..503287 (-) 978 WP_006178783.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54158.04 Da        Isoelectric Point: 4.3358

>NTDB_id=259135 CWI88_RS02425 WP_006178789.1 497421..498854(-) (rpoN/rpoN1) [Enterobacter cancerogenus strain CR-Eb1]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQTQDAEMLDTVEALEQKEMPD
ELPLDASWDELYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESIGDEDVELEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNSDSIPRLQINQQYASMCTSVRNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTLLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=259135 CWI88_RS02425 WP_006178789.1 497421..498854(-) (rpoN/rpoN1) [Enterobacter cancerogenus strain CR-Eb1]
ATGAAGCAAGGTTTGCAATTAAGGCTGAGCCAGCAACTGGCGATGACGCCGCAGCTCCAGCAGGCGATCCGTCTGCTGCA
GCTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGTTGCTGGAGCAAACCGATCTTCATG
ACGAGGTAGACACCCAGCAAACGCAAGACGCAGAAATGCTCGACACCGTTGAGGCGCTCGAACAAAAAGAGATGCCGGAC
GAGCTTCCGCTGGATGCCAGCTGGGATGAACTCTACACCGCCGGTACCCCTTCCGGAACGCGTGCAGACTACCAGGACGA
CGAGCTGCCGGTCTACCAGGGCGAAACCACCCAGTCTCTGCAGGATTACCTGATGTGGCAGGTGGAGCTTACGCCGTTCT
CCGATACCGATCGCGCCATTGCGACCTCCATTGTCGATGCCGTGGATGACACAGGCTATCTGACCGTCACGCTGGACGAC
ATTCTGGAAAGCATCGGCGACGAAGACGTTGAGCTGGAAGAAATTGAAGCCGTGTTAAAGCGCATCCAGCGCTTCGACCC
GGTCGGTGTGGCGGCGAAAGATCTGCGCGACTGCCTGCTGATCCAGCTTTCGCAGTTCGCCAAAGAGACACCGTGGATCG
ACGAAGCACGCTTAATCATCAGCGATCATCTGGATCTGCTCGCCAACCATGATTTCCGTTCGCTGATGCGCGTCACGCGT
CTGAAAGAAGAGGTGCTGAAAGAGGCGGTGAATTTAATTCAGTCCCTCGATCCGCGTCCGGGCCAGTCGATCCAGACCAG
CGAACCGGAATACGTGATCCCTGACGTGCTGGTGCGAAAGCATAACGGTCGCTGGGTGGTTGAACTGAATTCAGACAGCA
TCCCTCGTTTGCAAATTAACCAGCAGTACGCCTCCATGTGCACCAGCGTTCGCAACGACTCCGACAATCAATATATTCGT
AGCAACCTTCAGGAAGCGCGATGGTTGATCAAAAGTCTGGAGAGCCGGAATGATACGCTGCTGCGCGTAAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTCGAGCAGGGCGAAGAATTTATGAAGCCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAGTATCTGCACAGCCCGCGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAACACCGAAGGCGGCGGCGAAGCCTCGTCAACGGCAATCCGTGCACTGGTGAAGAAGTT
GATCGCCGCGGAAAACCCCGCGAAGCCACTGAGCGACAGTAAGTTGACCACCCTTCTCTCCGATCAGGGTATTATGGTGG
CACGTCGTACCGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.743

100

0.484

Multiple sequence alignment