Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   CXB69_RS00130 Genome accession   NZ_CP025206
Coordinates   24636..25343 (+) Length   235 a.a.
NCBI ID   WP_226474265.1    Uniprot ID   -
Organism   Latilactobacillus sakei strain WiKim0074     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 19636..30343
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CXB69_RS00100 (CXB69_00100) - 19845..21032 (+) 1188 WP_025015730.1 FtsW/RodA/SpoVE family cell cycle protein -
  CXB69_RS00105 (CXB69_00105) - 21043..22131 (+) 1089 WP_025015731.1 CAP-associated domain-containing protein -
  CXB69_RS00110 (CXB69_00110) - 22155..22445 (+) 291 WP_016265218.1 YlbG family protein -
  CXB69_RS00115 (CXB69_00115) rsmD 22450..23004 (+) 555 WP_112234018.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  CXB69_RS00120 (CXB69_00120) coaD 23017..23514 (+) 498 WP_025015732.1 pantetheine-phosphate adenylyltransferase -
  CXB69_RS00125 (CXB69_00125) - 23516..24562 (+) 1047 WP_035145546.1 SepM family pheromone-processing serine protease -
  CXB69_RS00130 (CXB69_00130) comEA 24636..25343 (+) 708 WP_226474265.1 helix-hairpin-helix domain-containing protein Machinery gene
  CXB69_RS00135 (CXB69_00135) - 25401..25889 (+) 489 WP_011374770.1 ComE operon protein 2 -
  CXB69_RS00140 (CXB69_00140) comEC 26087..28153 (+) 2067 WP_231953244.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  CXB69_RS00145 (CXB69_00145) holA 28150..29178 (+) 1029 WP_025015735.1 DNA polymerase III subunit delta -
  CXB69_RS00150 (CXB69_00150) rpsT 29246..29500 (-) 255 WP_011374767.1 30S ribosomal protein S20 -
  CXB69_RS00155 (CXB69_00155) rpsO 29779..30048 (+) 270 WP_011374766.1 30S ribosomal protein S15 -

Sequence

Protein


Download         Length: 235 a.a.        Molecular weight: 26122.75 Da        Isoelectric Point: 9.7683

>NTDB_id=258794 CXB69_RS00130 WP_226474265.1 24636..25343(+) (comEA) [Latilactobacillus sakei strain WiKim0074]
MMWQDYWQRYRRFWWVPCLVLLGIITGGFYWHQTQQKQKVTHNEQLLATSASKIKPATKKTHQAATKKKGGFVDIKGAVA
YPGIYPITVSETRLFEVLKNAGGPTDAADTKRLNLAQKLTDQLIIYIPKKGEVVTDQELIQAPQQIATTSERTPEQASTG
TSANASSASDTAKIDLNQADSTQLQTLNGIGPKKAEQIIAYRDEQGKFKQIEDLQKVGGIGPKTFEQLQSQICVN

Nucleotide


Download         Length: 708 bp        

>NTDB_id=258794 CXB69_RS00130 WP_226474265.1 24636..25343(+) (comEA) [Latilactobacillus sakei strain WiKim0074]
ATGATGTGGCAAGATTATTGGCAGCGATATCGGCGTTTTTGGTGGGTGCCTTGCTTAGTGCTATTGGGGATCATCACGGG
TGGCTTTTATTGGCATCAAACGCAACAAAAACAAAAAGTAACGCATAATGAGCAACTGCTAGCAACCAGTGCGTCTAAAA
TAAAACCTGCTACTAAGAAAACACACCAAGCGGCAACCAAGAAAAAAGGCGGTTTTGTCGATATAAAAGGTGCTGTGGCT
TATCCGGGAATTTATCCGATCACAGTCTCTGAAACGCGCTTATTTGAGGTGCTCAAAAATGCAGGTGGTCCGACTGATGC
GGCTGATACCAAGCGGCTTAATTTAGCCCAAAAATTAACCGATCAACTGATTATTTATATTCCGAAAAAAGGAGAGGTGG
TTACTGATCAAGAACTCATTCAAGCGCCACAACAAATTGCAACGACCAGTGAGCGTACGCCAGAACAAGCATCAACTGGG
ACAAGTGCGAATGCATCGAGCGCGAGTGACACTGCTAAAATCGATTTGAATCAGGCTGATAGTACGCAGCTTCAGACCCT
CAATGGCATTGGCCCCAAGAAAGCTGAACAAATAATTGCTTATCGTGATGAACAAGGAAAATTTAAACAGATTGAAGATC
TACAAAAAGTCGGCGGGATTGGTCCGAAGACGTTTGAACAATTGCAATCACAAATTTGTGTGAATTAG

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(73-124)

Helix-hairpin-helix motif (HHH_3)

(172-233)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Latilactobacillus sakei subsp. sakei 23K

97.021

100

0.97

Multiple sequence alignment