Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   CU648_RS08565 Genome accession   NZ_CP025122
Coordinates   1569115..1571454 (-) Length   779 a.a.
NCBI ID   WP_101194901.1    Uniprot ID   -
Organism   Bacillus sp. HBCD-sjtu     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1564115..1576454
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CU648_RS28465 (CU648_08540) - 1564983..1565159 (-) 177 WP_001242605.1 hypothetical protein -
  CU648_RS08545 (CU648_08545) - 1565245..1565436 (-) 192 WP_001104596.1 hypothetical protein -
  CU648_RS08550 (CU648_08550) - 1565703..1566143 (-) 441 WP_001266956.1 D-tyrosyl-tRNA(Tyr) deacylase -
  CU648_RS08555 (CU648_08555) relA 1566161..1568344 (-) 2184 WP_001262809.1 GTP diphosphokinase -
  CU648_RS08560 (CU648_08560) - 1568555..1569067 (-) 513 WP_000346215.1 adenine phosphoribosyltransferase -
  CU648_RS08565 (CU648_08565) recJ 1569115..1571454 (-) 2340 WP_101194901.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  CU648_RS08570 (CU648_08570) - 1571556..1572449 (-) 894 WP_000409725.1 cation diffusion facilitator family transporter -
  CU648_RS08575 (CU648_08575) secDF 1572603..1574867 (-) 2265 WP_001119073.1 protein translocase subunit SecDF -
  CU648_RS08580 (CU648_08580) - 1575138..1575422 (-) 285 WP_000886980.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 779 a.a.        Molecular weight: 88090.69 Da        Isoelectric Point: 4.9330

>NTDB_id=258487 CU648_RS08565 WP_101194901.1 1569115..1571454(-) (recJ) [Bacillus sp. HBCD-sjtu]
MLQPKTRWKEKEYNGERVSELASKLQLSPLVVSLFLGRGLDTEDKILDFLNTENQEFHDPFLLEGMDRTVERVNKAIQNG
EQILIFGDYDADGVSSTTVLYLALQELGADVEFYIPNRFTEGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEAKVAKEL
GVDLIITDHHEPPPELPEALAIIHPKLDGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VKRGLKHMRMTKNIGLKALFKVANVSQSEITEESIGFSIAPRINAVGRLEDATPAVHLLLSEDPEEAKELAEEIDELNKL
RKDIVKQITEEAIAEVETNFPPEDNKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLCIDPVKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLHMNDVDELRRRLNEQAEVILTEEDFIPITTVDALCKVEDVTLAAIEDMQKLAPFGV
GNPKPRIAVKDAELESIRAIGSDGSHLKMALRDGQATLDTIGFGFGAYAKEISPVAKVSVIGEASINEWNNFKKPQLMVQ
DIAVEAWQLFDWRSMRNVEANVAELPKEKITMVYFSKEVLHKFSLEDYKEHMMHASEVTELDEQYIVLLDLPKGTDELRD
LFKVGFPSRIYTLFYQENNHLFSTVPTRDHFKWYYSFLSQKSPFSLRQYGEQLCQHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMADKKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHKEVEQLG

Nucleotide


Download         Length: 2340 bp        

>NTDB_id=258487 CU648_RS08565 WP_101194901.1 1569115..1571454(-) (recJ) [Bacillus sp. HBCD-sjtu]
GTGTTACAACCGAAGACGCGTTGGAAAGAAAAAGAATATAATGGCGAGCGAGTGAGTGAATTAGCAAGTAAATTACAATT
ATCACCACTTGTCGTTTCGTTATTCCTCGGTAGAGGACTAGATACAGAAGATAAGATTTTAGATTTTTTAAATACAGAAA
ATCAAGAATTTCATGATCCATTTTTATTAGAAGGAATGGATAGAACGGTAGAACGTGTAAATAAGGCGATTCAAAATGGA
GAGCAAATATTAATATTTGGTGACTACGATGCGGACGGAGTAAGTAGTACGACTGTATTATATCTTGCCCTACAAGAATT
AGGTGCAGACGTAGAATTTTATATCCCAAACCGTTTTACAGAAGGTTATGGACCAAACGAAGAAGCATTCCGTTGGGCGC
ATAGCGCAGGATTCTCACTCATTATTACTGTTGATACTGGTATCGCAGCTGTACATGAAGCGAAAGTAGCGAAGGAACTT
GGGGTAGATCTTATTATTACGGATCACCATGAGCCGCCACCAGAATTGCCAGAAGCACTTGCGATTATTCATCCGAAATT
AGATGGCGGTGTGTATCCGTTTCACTATTTAGCCGGCGTTGGTGTTGCGTTTAAAGTTGCGCATGCACTACTGGGCCGTG
TACCAGAACATTTATTAGAAATTGCAGTAATTGGTACAGTAGCCGATTTAGTATCACTTCACGGTGAAAATAGATTGCTA
GTGAAGCGCGGTTTAAAACATATGCGCATGACGAAAAATATTGGACTAAAGGCGCTATTTAAAGTAGCTAACGTTTCGCA
AAGTGAAATTACAGAAGAGAGTATTGGCTTCTCAATCGCACCTCGTATTAATGCAGTTGGACGTTTAGAAGATGCTACGC
CGGCTGTACATCTTTTATTATCAGAGGATCCGGAAGAAGCGAAAGAGCTAGCTGAAGAAATTGATGAGTTGAACAAACTG
CGAAAAGATATTGTAAAGCAAATTACAGAAGAAGCTATCGCTGAAGTGGAAACGAATTTCCCGCCTGAAGACAATAAAGT
ATTAGTGCTTGCAAAAGAAGGCTGGAATCCAGGTGTAATTGGAATTGTCGCTTCTAAATTAGTTGAACGTTTTTATCGTC
CAACGATTGTATTATGCATTGATCCTGTAAAAGAAACTGCAAAAGGTTCAGCGCGTAGTATTGCAGGATTCGATTTGTTT
GCAAACTTGTCAGATTGTAGAGAGTTATTGCCACACTTCGGGGGACATCCGATGGCAGCGGGAATGACGCTACATATGAA
TGATGTAGATGAATTACGCCGCCGTTTAAATGAGCAAGCGGAGGTAATTTTAACGGAAGAAGATTTTATCCCAATTACAA
CTGTCGATGCTTTGTGTAAAGTGGAGGACGTAACACTTGCAGCAATCGAGGATATGCAAAAGTTGGCACCATTTGGTGTT
GGAAATCCAAAGCCACGTATTGCGGTGAAGGATGCAGAGTTAGAAAGTATTCGTGCAATCGGATCAGACGGTTCTCATTT
AAAAATGGCTCTTCGTGATGGACAGGCAACACTTGATACAATCGGGTTTGGATTTGGAGCGTACGCGAAAGAAATTTCTC
CAGTTGCAAAGGTATCTGTAATCGGGGAAGCGTCTATCAATGAATGGAATAATTTTAAGAAGCCACAATTAATGGTACAA
GATATTGCTGTAGAGGCATGGCAATTATTTGATTGGCGTAGTATGCGTAACGTAGAAGCAAATGTAGCAGAACTTCCGAA
AGAAAAAATAACGATGGTGTATTTTTCTAAAGAGGTATTGCATAAATTTTCTTTAGAAGACTATAAAGAGCATATGATGC
ATGCATCAGAAGTAACCGAGTTAGATGAGCAATACATCGTGTTACTTGATTTACCAAAAGGAACAGATGAATTACGAGAT
CTATTTAAAGTTGGGTTCCCGTCGCGAATTTATACGCTATTTTATCAAGAGAATAATCATTTATTTAGTACTGTTCCAAC
GAGGGATCACTTTAAATGGTACTATTCATTCTTGAGCCAAAAATCACCATTTTCTTTAAGACAATATGGAGAACAACTAT
GTCAACATAAAGGTTGGTCAAAAGATACAGTAAATTTCATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GATGGCGTCATTTTTATGGCAGACAAAAAACAAAAGCGTGATTTAATTGAATCGAACACATATCGTGAGAAAATGAACCA
CTTACAATTAGAAAAAGAGTTGGTTTATAGCACATATCAGCAACTATATACATGGTTTGAAACGATTCGTAATCATAAAG
AAGTAGAACAGCTAGGGTAA

Domains

Predicted by InterproScan.

DHH family (DHH)

(82-226)

DHHA1 domain (DHHA1)

(315-439)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)

RecJ OB domain (RecJ_OB)

(455-561)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

52.972

99.358

0.526

Multiple sequence alignment