Detailed information NTG0258046

Bioinformatically predicted

Overview

• Name: recD2
• Type: Machinery gene
• Locus tag: CWI35_RS15490
• Genome accession: NZ_CP025074
• Coordinates: 2999755..3002115 (+)
• Length: 786 a.a.
• NCBI ID: WP_011232038.1
• Uniprot ID: Q5KWU0
• Organism: [Bacillus] caldolyticus strain NEB414
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2994755..3007115

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CWI35_RS15465 (CWI35_15435)	-	2996022..2996246 (+)	225	WP_011232043.1	hypothetical protein	-
CWI35_RS15470 (CWI35_15440)	-	2996266..2996682 (+)	417	WP_011232042.1	Rrf2 family transcriptional regulator	-
CWI35_RS15475 (CWI35_15445)	-	2996710..2997852 (+)	1143	WP_011232041.1	cysteine desulfurase family protein	-
CWI35_RS15480 (CWI35_15450)	mnmA	2997867..2998982 (+)	1116	WP_119877911.1	tRNA 2-thiouridine(34) synthase MnmA	-
CWI35_RS15485 (CWI35_15455)	-	2999073..2999735 (+)	663	WP_011232039.1	tetratricopeptide repeat protein	-
CWI35_RS15490 (CWI35_15460)	recD2	2999755..3002115 (+)	2361	WP_011232038.1	ATP-dependent RecD-like DNA helicase	Machinery gene
CWI35_RS15495 (CWI35_15465)	-	3002141..3002614 (+)	474	WP_011232037.1	PRC-barrel domain-containing protein	-
CWI35_RS15500 (CWI35_15470)	-	3002630..3002821 (+)	192	WP_008881018.1	hypothetical protein	-
CWI35_RS15505 (CWI35_15475)	-	3002833..3002967 (+)	135	WP_013144658.1	YrzQ family protein	-
CWI35_RS15510 (CWI35_15480)	-	3003086..3004147 (+)	1062	WP_021321998.1	AI-2E family transporter	-
CWI35_RS15515 (CWI35_15485)	alaS	3004466..3007102 (+)	2637	WP_014196399.1	alanine--tRNA ligase	-

Sequence

Protein

• Length: 786 a.a.
• Molecular weight: 86551.19 Da
• Isoelectric Point: 5.0447

>NTDB_id=258046 CWI35_RS15490 WP_011232038.1 2999755..3002115(+) (recD2) [[Bacillus] caldolyticus strain NEB414]
MQEQQILALDGRTFIKGVCLAVIFHNEETLYTVVRVRVEETNEAPVEEEVVVTGYFPRLNEGDVYIFYGQFRQHPRFGRQ
YAASQFRKQLPNTKEGVIHYLSSGLFKGIGKKTAAAIVETLGENAIAAILRDPRALERVPKLTKKKAKQLYDALRAHEGL
EQTMIALAQLGFGPQLAMKIYQAYGEEAVDIIHENPYQLVEDIEGIGFGRADEIGRQLGISGSHPARLRAASLFVLEQAC
LQEGHVYLREEELMERIGELLDGRLSGAVDGRAISQTLLMLSEEGKLIAEQGRYYLPSLYFAEKGIVSNIKRLLGQTPPS
AFAESEFLLALGALEERLGMQYAPQQREAIQQALSSPLFILTGGPGTGKTTVIKGIVELFAALNGLSLDPADYKQGEPFP
VLLAAPTGRAAKRMSEATGLPAATIHRLLGWNGAEGFSRDENEPISGKLLVIDEMSMVDTWLANQLLKAVPNGMQVIFVG
DEDQLPSVGPGQVLKDLLSSGVIPFARLTEVYRQAEGSSIIELAHEMKRGVVPDDLTAPKADRSFIRCRAGQVVDVVRQI
ADNARKKGFSVKDIQVLAPMYRGPAGIDQLNKALQELFNPPAEGKRELSVGEVVYRVGDKVLQLVNQPEDNVFNGDIGEI
VAIFYAKENIEKQDLLVVSFDGIEVTYTRQDLGQITHAYCCSVHKAQGSEFPIVILPVVKSYYRMLKRNLLYTAVTRSKQ
FLVLCGEEEAFRFGVAKQSDGARQTALAEKLAGAAAPFVEVELPFEEANMGMENVTPYDFMGDEPS

Nucleotide

• Length: 2361 bp

>NTDB_id=258046 CWI35_RS15490 WP_011232038.1 2999755..3002115(+) (recD2) [[Bacillus] caldolyticus strain NEB414]
TTGCAAGAGCAACAAATCCTCGCCCTTGACGGACGCACGTTCATTAAAGGAGTGTGCTTGGCGGTCATTTTTCATAATGA
GGAAACATTGTACACGGTCGTCCGCGTGCGCGTTGAGGAAACGAACGAAGCGCCCGTTGAGGAGGAAGTCGTCGTCACCG
GCTATTTTCCGCGTTTAAACGAAGGGGACGTCTACATCTTTTACGGACAATTCCGCCAACATCCGCGTTTTGGCCGCCAA
TATGCGGCTAGTCAGTTCCGCAAACAGCTTCCGAACACGAAAGAAGGAGTCATTCATTATTTATCGAGCGGTTTGTTTAA
AGGAATCGGCAAAAAAACGGCTGCGGCGATTGTCGAGACGCTCGGCGAAAACGCGATCGCCGCGATTTTGCGCGACCCCA
GGGCGCTAGAGCGTGTGCCGAAGCTGACGAAAAAAAAGGCGAAGCAGCTGTACGATGCGCTTCGCGCCCACGAAGGGCTT
GAGCAAACGATGATCGCCCTCGCCCAGCTCGGTTTTGGCCCGCAGCTGGCCATGAAAATTTACCAAGCGTATGGCGAAGA
AGCGGTTGACATCATCCACGAGAACCCGTACCAGCTCGTTGAGGATATCGAAGGGATCGGCTTTGGCCGCGCTGATGAAA
TCGGGCGCCAGCTCGGCATTTCCGGCAGCCATCCGGCCCGGCTGCGCGCCGCCTCGTTGTTTGTTCTTGAACAGGCGTGC
TTGCAGGAAGGGCACGTTTATTTGCGCGAGGAAGAGCTGATGGAGCGCATCGGGGAGCTGCTTGACGGAAGGCTGAGCGG
TGCGGTGGATGGACGAGCGATCAGCCAAACGCTTCTGATGTTAAGCGAAGAAGGGAAGCTGATCGCCGAGCAAGGGCGTT
ATTATCTCCCTTCCCTTTACTTCGCCGAGAAAGGGATCGTCTCCAACATTAAGCGGCTGCTCGGGCAGACGCCGCCGTCG
GCGTTTGCCGAGTCGGAGTTTTTGCTGGCGCTTGGGGCGCTTGAGGAACGGCTTGGCATGCAATATGCCCCCCAGCAAAG
GGAAGCGATCCAACAAGCGCTTTCTTCGCCGCTGTTTATTTTGACCGGCGGTCCGGGAACGGGGAAAACGACGGTCATCA
AAGGCATCGTCGAGCTGTTTGCCGCCCTAAACGGCCTGTCTCTTGACCCGGCTGACTACAAACAAGGCGAGCCGTTTCCG
GTGCTGCTGGCCGCACCGACCGGCCGAGCGGCGAAGCGGATGAGCGAGGCGACCGGGCTGCCGGCGGCGACGATCCACCG
CTTGCTTGGCTGGAATGGGGCGGAAGGGTTCAGCCGCGACGAGAACGAACCGATTTCCGGCAAGCTGCTTGTCATCGATG
AGATGTCGATGGTCGACACGTGGCTCGCCAACCAGCTGTTAAAAGCCGTGCCAAACGGCATGCAAGTCATTTTTGTCGGC
GATGAGGATCAATTGCCATCCGTCGGGCCAGGGCAAGTGCTGAAAGACTTGCTCTCTTCAGGCGTCATTCCGTTCGCTCG
GTTGACCGAGGTGTATCGCCAGGCGGAAGGATCATCGATCATCGAGCTTGCTCATGAGATGAAGCGCGGCGTCGTGCCGG
ACGATTTGACCGCTCCGAAGGCAGACCGTTCATTCATCCGCTGCCGTGCGGGCCAGGTCGTCGATGTCGTGCGGCAAATC
GCGGACAACGCGCGCAAAAAAGGGTTTTCCGTCAAAGATATTCAAGTGCTCGCCCCGATGTACCGTGGTCCAGCGGGCAT
TGACCAATTGAACAAAGCGCTTCAAGAGCTGTTCAACCCGCCGGCTGAGGGGAAGCGGGAGCTGTCGGTCGGTGAGGTCG
TCTACCGCGTCGGCGATAAGGTGCTGCAGCTCGTCAATCAACCGGAAGACAATGTGTTTAACGGCGACATCGGCGAAATC
GTTGCCATTTTTTACGCCAAAGAAAATATCGAAAAGCAAGACTTGCTCGTCGTCTCGTTTGATGGCATTGAAGTGACGTA
CACACGGCAAGATTTAGGACAAATCACCCATGCGTACTGCTGTTCGGTTCATAAGGCGCAAGGAAGCGAGTTTCCAATCG
TCATTTTGCCCGTCGTCAAAAGCTATTACCGGATGTTGAAACGGAATTTGTTGTATACTGCGGTGACAAGAAGCAAGCAG
TTTCTCGTTTTATGCGGGGAAGAGGAAGCGTTTCGATTCGGCGTCGCAAAACAAAGCGACGGAGCGCGCCAGACGGCGCT
CGCTGAGAAGCTGGCGGGGGCGGCGGCGCCGTTTGTCGAGGTCGAACTGCCGTTTGAAGAGGCGAACATGGGGATGGAAA
ACGTTACGCCGTATGACTTTATGGGCGATGAGCCAAGCTAA

Domains

Predicted by InterproScan.

• AAA domain (AAA_30) (341-548)

• Helix-hairpin-helix domain (HHH_5) (107-155)

• ATP-dependent RecD-like DNA helicase SH3 domain (SH3_13) (590-660)

• Helix-hairpin-helix containing domain (HHH_RecD2) (157-247)

• UvrD-like helicase C-terminal domain (UvrD_C_2) (677-725)

3D structure

• AlphaFold DB: Q5KWU0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recD2	Bacillus subtilis subsp. subtilis str. 168	59.975	100	0.604