Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   CWI35_RS15395 Genome accession   NZ_CP025074
Coordinates   2976587..2978953 (+) Length   788 a.a.
NCBI ID   WP_047758190.1    Uniprot ID   A0A2Z3NDG0
Organism   [Bacillus] caldolyticus strain NEB414     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2971587..2983953
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWI35_RS15380 (CWI35_15350) - 2972832..2973119 (+) 288 WP_013524218.1 post-transcriptional regulator -
  CWI35_RS15385 (CWI35_15355) secDF 2973232..2975484 (+) 2253 WP_119877910.1 protein translocase subunit SecDF -
  CWI35_RS15390 (CWI35_15360) - 2975621..2976529 (+) 909 WP_082218536.1 cation diffusion facilitator family transporter -
  CWI35_RS15395 (CWI35_15365) recJ 2976587..2978953 (+) 2367 WP_047758190.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  CWI35_RS15400 (CWI35_15370) - 2978944..2979456 (+) 513 WP_013144641.1 adenine phosphoribosyltransferase -
  CWI35_RS15405 (CWI35_15375) - 2979605..2981803 (+) 2199 WP_011232055.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  CWI35_RS15410 (CWI35_15380) dtd 2981827..2982285 (+) 459 WP_011232054.1 D-aminoacyl-tRNA deacylase -
  CWI35_RS18340 - 2982367..2982540 (+) 174 WP_020756192.1 hypothetical protein -
  CWI35_RS15415 (CWI35_15385) ahpC 2982775..2983338 (+) 564 WP_011232052.1 alkyl hydroperoxide reductase subunit C -

Sequence

Protein


Download         Length: 788 a.a.        Molecular weight: 86829.12 Da        Isoelectric Point: 5.7225

>NTDB_id=258045 CWI35_RS15395 WP_047758190.1 2976587..2978953(+) (recJ) [[Bacillus] caldolyticus strain NEB414]
MLKAKTRWEVERLDEQAVRRLAEEAGVTPLLARLLVRRGVRTASEAAAFLNPLEQPFHDPFLLDGMERAIQRLKRAIDAG
ERVLVYGDYDADGVCSTSVMVSALKEAGASVEFYIPNRFTEGYGPNMAAFRSAKERGVSVIVTVDNGIAAVKEIAAANEW
GMDVIVTDHHEPGPVLPEAHAIIHPKQPGSTYPFRDLAGVGVAFKVAHALLGRVPRHLLDLVAIGTIADLVPLVGENRLF
VAHGLEALRATERIGLRALFRQCRIDAAAINEQTVGFVIAPRLNAAGRLGGADPAVALLMTDDENEAVQLAAEMDELNRE
RQQLVAQIAEEAADMVRRQFPPEEHRVLVVAGEGWNAGVVGIVASKLVEQFYRPAVVLSIDREKGIAKGSARSIHGFDLF
ASLSQCRDLLPHFGGHPMAAGMTLSLDDVEELRQRLNDIAAETLSEDDFTPPTFIDASCSIAELTLSAARSLERFAPFGV
GNPRPLVLIEGASVETMRRVGANGAHMKAVFSQDGAAIDAIGFGLGPLCEEIAPDARISAVGELSVNEWNGFAKPQLSLC
DLAVSDCQLFDVRGCRDVRPLLERLPKEKRLVVSFRCETGQRPDVAPFRGELHCVPTEEEAGALSIDDRYVVLLDVPPRL
ALLSALLGSGRPARIYAVFAQPEAQFFRTFPTRDHFKWFYAFLHQHRSFPLAKRGGELARARGWTEETVHFMAKVFLELD
FINEQNGVISLVHQPAKRDLHESPTYRRRRDELEAEHQLVYSTYEQLKRCLAEMTRSQTHEEANVSWT

Nucleotide


Download         Length: 2367 bp        

>NTDB_id=258045 CWI35_RS15395 WP_047758190.1 2976587..2978953(+) (recJ) [[Bacillus] caldolyticus strain NEB414]
ATGTTGAAAGCGAAAACGCGTTGGGAGGTGGAGCGCCTCGATGAGCAGGCGGTGAGGCGGCTGGCTGAGGAAGCCGGCGT
CACGCCCTTATTGGCGAGGCTTCTCGTCCGCCGCGGGGTGCGGACGGCCTCGGAGGCGGCGGCGTTTCTCAATCCGCTTG
AGCAACCGTTTCATGATCCGTTTTTGCTTGATGGGATGGAGCGAGCGATTCAGCGTCTAAAGCGGGCGATCGACGCGGGT
GAACGCGTGTTGGTATACGGCGATTACGATGCCGATGGCGTTTGCAGCACGTCGGTGATGGTGAGTGCTCTCAAGGAGGC
GGGGGCGTCGGTCGAGTTCTACATTCCGAACCGATTTACGGAAGGATACGGGCCGAACATGGCGGCGTTTCGCTCCGCAA
AAGAGCGCGGCGTTTCCGTCATTGTGACCGTCGACAATGGGATTGCGGCGGTCAAGGAAATTGCGGCCGCCAACGAGTGG
GGGATGGATGTCATCGTAACGGATCACCACGAGCCAGGTCCGGTGTTGCCGGAGGCGCATGCGATCATTCATCCGAAGCA
GCCGGGAAGTACATATCCGTTTCGCGATTTAGCCGGCGTCGGCGTTGCGTTTAAGGTAGCCCATGCCTTGCTTGGGCGGG
TGCCGCGCCATTTGCTTGATTTGGTCGCGATCGGCACGATCGCTGATTTGGTTCCGCTTGTTGGGGAAAACCGCCTCTTT
GTTGCACACGGGCTTGAAGCGTTGCGAGCGACGGAGCGCATCGGGCTGCGCGCTCTTTTTCGGCAATGCCGCATTGATGC
GGCGGCCATCAACGAACAGACGGTCGGATTTGTCATCGCTCCGCGCTTGAACGCGGCGGGGCGCCTCGGCGGCGCCGATC
CGGCAGTGGCGCTCTTAATGACGGACGATGAGAACGAAGCGGTGCAGCTGGCGGCGGAGATGGACGAGTTAAACCGCGAA
CGTCAGCAGCTTGTGGCACAAATCGCTGAAGAAGCGGCGGACATGGTGCGCCGCCAATTTCCTCCTGAGGAACATCGCGT
CTTGGTTGTGGCAGGCGAAGGATGGAATGCCGGAGTGGTGGGCATTGTCGCTTCTAAGCTTGTCGAGCAGTTTTACCGCC
CGGCGGTCGTGCTTTCAATCGACCGGGAAAAAGGGATCGCCAAAGGGTCAGCCCGCAGCATTCATGGGTTTGACTTATTC
GCCAGCCTGTCGCAATGCCGCGACCTGTTGCCGCACTTTGGCGGCCACCCGATGGCGGCGGGGATGACATTGTCGCTTGA
TGATGTCGAAGAATTACGGCAACGGCTGAACGACATCGCCGCCGAAACGTTGTCTGAGGACGATTTTACGCCGCCGACGT
TCATTGATGCTTCCTGTTCGATTGCTGAGTTGACGCTGTCGGCCGCCCGCAGCCTTGAGCGGTTCGCTCCGTTTGGCGTC
GGCAATCCGCGGCCGCTTGTTCTCATTGAAGGGGCGTCCGTTGAAACCATGCGGCGCGTCGGGGCGAACGGAGCCCATAT
GAAAGCCGTCTTTTCCCAAGACGGGGCCGCGATCGATGCCATCGGCTTCGGCCTTGGGCCGTTATGCGAAGAAATCGCTC
CGGATGCGCGCATTTCGGCGGTCGGGGAGCTGTCGGTGAACGAATGGAACGGGTTCGCCAAGCCGCAGCTGTCGTTGTGT
GATCTGGCGGTCAGCGACTGCCAGCTGTTTGACGTCCGCGGCTGCCGCGACGTCCGTCCACTTCTTGAGCGGCTGCCAAA
AGAGAAGCGGCTAGTCGTCTCCTTTCGCTGTGAAACTGGGCAGCGCCCGGACGTGGCGCCATTTCGCGGGGAGCTTCATT
GCGTGCCGACCGAAGAAGAGGCGGGCGCCTTATCGATTGACGACCGCTATGTCGTGCTGCTTGACGTGCCGCCGCGGCTG
GCCTTGCTGTCGGCGCTTCTTGGCAGCGGGCGGCCGGCGCGCATTTACGCGGTGTTTGCCCAGCCCGAGGCGCAATTTTT
CCGCACGTTTCCAACGCGCGACCATTTTAAATGGTTTTACGCCTTTTTGCATCAACACCGTTCGTTTCCGCTCGCCAAGC
GCGGTGGTGAGCTGGCCCGCGCCCGCGGTTGGACGGAAGAGACGGTTCATTTTATGGCGAAAGTATTTTTAGAGCTTGAC
TTTATCAACGAACAAAACGGCGTCATTTCGCTCGTTCATCAGCCGGCGAAGCGCGATTTGCACGAATCGCCGACGTACCG
CCGGCGGCGGGACGAGCTCGAGGCGGAGCATCAGTTAGTCTATTCCACTTACGAGCAATTGAAACGCTGTTTGGCGGAAA
TGACACGTTCGCAAACGCACGAGGAGGCAAACGTATCATGGACTTAA

Domains

Predicted by InterproScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-771)

DHHA1 domain (DHHA1)

(315-439)

DHH family (DHH)

(82-226)

RecJ OB domain (RecJ_OB)

(455-560)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2Z3NDG0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

53.017

98.858

0.524

Multiple sequence alignment