Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   DCS61_RS20655 Genome accession   NZ_CP029707
Coordinates   4256358..4256783 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain K34-7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4251358..4261783
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DCS61_RS20640 (DCS61_20640) pilX 4251922..4252509 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  DCS61_RS20645 (DCS61_20645) pilY1 4252521..4256012 (+) 3492 WP_003102607.1 type 4a pilus biogenesis protein PilY1 -
  DCS61_RS20650 (DCS61_20650) pilY2 4256014..4256361 (+) 348 WP_003161774.1 type 4a fimbrial biogenesis protein PilY2 -
  DCS61_RS20655 (DCS61_20655) comF 4256358..4256783 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  DCS61_RS20660 (DCS61_20660) ispH 4256830..4257774 (-) 945 WP_003134939.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  DCS61_RS20665 (DCS61_20665) fkpB 4257860..4258300 (-) 441 WP_003161775.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  DCS61_RS20670 (DCS61_20670) lspA 4258293..4258802 (-) 510 WP_003102615.1 signal peptidase II -
  DCS61_RS20675 (DCS61_20675) ileS 4258795..4261626 (-) 2832 WP_003102617.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=257196 DCS61_RS20655 WP_003094721.1 4256358..4256783(+) (comF) [Pseudomonas aeruginosa strain K34-7]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=257196 DCS61_RS20655 WP_003094721.1 4256358..4256783(+) (comF) [Pseudomonas aeruginosa strain K34-7]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGCTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAACTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACACCCACCAGCACTACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383