Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   CSC33_RS18850 Genome accession   NZ_CP029660
Coordinates   3938746..3939171 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain AR_0446     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3933746..3944171
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CSC33_RS18835 (CSC33_3818) pilX 3934316..3934903 (+) 588 WP_033972921.1 type 4a pilus minor pilin PilX -
  CSC33_RS18840 (CSC33_3819) pilY1 3934915..3938400 (+) 3486 WP_110194331.1 type 4a pilus biogenesis protein PilY1 -
  CSC33_RS18845 (CSC33_3820) pilY2 3938402..3938749 (+) 348 WP_033972924.1 type 4a fimbrial biogenesis protein PilY2 -
  CSC33_RS18850 (CSC33_3821) comF 3938746..3939171 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  CSC33_RS18855 (CSC33_3822) ispH 3939218..3940162 (-) 945 WP_003134939.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  CSC33_RS18860 (CSC33_3823) fkpB 3940248..3940688 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  CSC33_RS18865 (CSC33_3824) lspA 3940681..3941190 (-) 510 WP_025271359.1 signal peptidase II -
  CSC33_RS18870 (CSC33_3825) ileS 3941183..3944014 (-) 2832 WP_110194332.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=255937 CSC33_RS18850 WP_003094721.1 3938746..3939171(+) (comF) [Pseudomonas aeruginosa strain AR_0446]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=255937 CSC33_RS18850 WP_003094721.1 3938746..3939171(+) (comF) [Pseudomonas aeruginosa strain AR_0446]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGCTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCGCTGCTCTCGGACGCGGCCGCTCGCC
AGGAACGCTACTATTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383