Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   CUC43_RS03115 Genome accession   NZ_CP024771
Coordinates   541198..543537 (-) Length   779 a.a.
NCBI ID   WP_061687094.1    Uniprot ID   -
Organism   Bacillus thuringiensis LM1212     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 536198..548537
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CUC43_RS33550 (CUC43_03205) - 537423..537599 (-) 177 WP_001242605.1 hypothetical protein -
  CUC43_RS03100 (CUC43_03215) - 537787..538227 (-) 441 WP_029439998.1 D-tyrosyl-tRNA(Tyr) deacylase -
  CUC43_RS03105 (CUC43_03220) relA 538245..540428 (-) 2184 WP_029439999.1 GTP diphosphokinase -
  CUC43_RS03110 (CUC43_03225) - 540638..541150 (-) 513 WP_000346214.1 adenine phosphoribosyltransferase -
  CUC43_RS03115 (CUC43_03230) recJ 541198..543537 (-) 2340 WP_061687094.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  CUC43_RS03120 (CUC43_03235) - 543639..544532 (-) 894 WP_029440000.1 cation diffusion facilitator family transporter -
  CUC43_RS03125 (CUC43_03240) secDF 544687..546951 (-) 2265 WP_029440001.1 protein translocase subunit SecDF -
  CUC43_RS03130 (CUC43_03245) - 547221..547505 (-) 285 WP_029440002.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 779 a.a.        Molecular weight: 88196.73 Da        Isoelectric Point: 4.8444

>NTDB_id=255347 CUC43_RS03115 WP_061687094.1 541198..543537(-) (recJ) [Bacillus thuringiensis LM1212]
MLQPKTRWKEKEYNGERVSELASKLQLSPLVVSLFLGRGLDTEDKILDFLNTENQEFHDPFLLEGMDRTVERVNKAIQNG
EQILIFGDYDADGVSSTTVLYLALQELGADVEFYIPNRFTEGYGPNEDAFRWAHSAGFSLIITVDTGIAAVHEAKVAKEL
GIDLIITDHHEPPPELPEALAIIHPKLDGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VKRGLKHMRMTKNIGLKALFKVANVSQSEITEESIGFSIAPRINAVGRLEDATPAVHLLLSEDPEEAKELAEEIDELNKL
RKDIVKQITEEAIAEVENNFPPEENKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLCIDPVKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLHMNDVDELRRRLNEQAEVILTEEDFIPITAVDAFCKVEDVTLAAIEDMQKLAPFGV
GNPKPRIAVKDAELESIRAIGSDGSHLKMALRDGQATLDTIGFGFGAYAKEISPVAKVSVIGEASINEWNNFKKPQLMVQ
DIAVEAWQLFDWRSMRNVEANVAELPKEKITMVYFSEEVLHKFSLEDYKEYMMHASEVTELDDQYIVFLDLPKGTDELRE
LFKVGFPSRIYTLFYQENNHLFSTVPTREHFKWYYSFLSQKSPFSLRQYGEQLCQHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMADKKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHKEVEQLG

Nucleotide


Download         Length: 2340 bp        

>NTDB_id=255347 CUC43_RS03115 WP_061687094.1 541198..543537(-) (recJ) [Bacillus thuringiensis LM1212]
GTGTTACAACCGAAGACGCGTTGGAAAGAAAAAGAATATAATGGCGAGCGAGTGAGTGAATTAGCAAGTAAATTACAATT
ATCACCACTTGTCGTTTCGTTATTCCTCGGTAGAGGACTAGATACAGAAGATAAGATTTTAGATTTTTTAAATACAGAAA
ATCAAGAATTTCATGATCCATTTTTATTAGAAGGAATGGATAGAACGGTAGAACGTGTAAATAAGGCGATTCAAAATGGA
GAGCAAATATTAATATTTGGTGACTACGATGCGGACGGAGTAAGTAGTACGACTGTGTTATATCTTGCCTTGCAAGAATT
AGGTGCAGATGTAGAATTTTATATTCCAAACCGTTTTACAGAAGGATATGGACCAAACGAAGACGCATTTCGTTGGGCAC
ATAGCGCAGGATTCTCATTAATTATTACTGTCGATACTGGTATCGCAGCTGTACATGAAGCGAAAGTAGCGAAGGAACTT
GGGATAGATCTAATTATTACAGATCACCATGAGCCACCACCAGAATTGCCAGAAGCACTTGCAATTATTCACCCGAAATT
AGATGGCGGTGTGTATCCGTTTCACTATTTAGCGGGCGTTGGTGTTGCGTTTAAAGTTGCGCATGCACTACTGGGCCGTG
TACCAGAACATTTATTAGAAATTGCAGTAATTGGTACAGTGGCTGATTTAGTATCACTTCATGGTGAGAATAGATTGCTA
GTGAAGCGCGGTTTAAAACATATGCGCATGACGAAAAATATCGGACTAAAGGCGCTATTTAAAGTTGCTAACGTTTCGCA
AAGTGAAATTACAGAAGAGAGTATTGGCTTCTCAATCGCACCTCGTATTAATGCAGTTGGACGTTTAGAAGATGCTACGC
CGGCTGTACATCTTTTATTATCAGAGGATCCGGAAGAAGCGAAAGAGTTGGCTGAAGAAATTGATGAGCTAAACAAACTG
CGAAAAGATATTGTAAAGCAAATTACAGAAGAAGCTATCGCTGAAGTGGAGAATAATTTCCCGCCAGAAGAAAATAAAGT
ATTAGTGCTTGCGAAAGAAGGTTGGAATCCAGGTGTAATTGGAATTGTCGCTTCTAAATTAGTTGAACGTTTTTATCGTC
CAACGATTGTATTATGCATTGATCCTGTAAAAGAAACTGCAAAAGGTTCAGCGCGTAGTATTGCAGGATTCGATTTGTTT
GCGAACTTGTCAGATTGTAGAGAGTTATTGCCACACTTCGGAGGACATCCGATGGCAGCGGGAATGACGCTACATATGAA
TGATGTAGATGAATTGCGCCGCCGTTTAAATGAGCAAGCAGAGGTAATTTTAACAGAAGAAGATTTTATTCCAATTACAG
CTGTCGATGCTTTTTGTAAAGTGGAGGACGTAACACTTGCAGCAATTGAAGATATGCAAAAGTTAGCGCCATTTGGTGTT
GGAAATCCAAAGCCACGTATTGCGGTGAAGGATGCAGAGTTAGAAAGTATTCGTGCAATCGGATCAGACGGTTCTCATTT
AAAAATGGCTCTTCGTGATGGACAAGCAACATTGGATACAATTGGATTCGGTTTTGGCGCATATGCAAAAGAAATCTCTC
CAGTTGCAAAAGTCTCTGTAATTGGGGAAGCGTCTATTAATGAATGGAATAATTTTAAGAAGCCACAACTAATGGTACAA
GATATTGCTGTAGAGGCATGGCAATTATTTGATTGGCGCAGTATGCGTAACGTAGAAGCAAATGTAGCAGAACTTCCGAA
AGAAAAAATAACGATGGTGTATTTTTCTGAAGAGGTATTGCATAAATTTTCTTTAGAAGACTATAAAGAGTATATGATGC
ATGCATCAGAAGTAACAGAATTAGATGATCAATACATCGTGTTCCTTGATTTACCAAAAGGAACAGATGAATTACGAGAG
CTATTTAAAGTAGGGTTCCCTTCACGAATCTATACACTATTTTATCAAGAGAATAATCATTTATTTAGTACTGTTCCAAC
GAGAGAACACTTTAAATGGTATTATTCGTTCTTGAGCCAAAAATCACCATTTTCTTTAAGACAATATGGAGAACAACTAT
GTCAACATAAAGGTTGGTCAAAAGATACAGTAAATTTCATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GATGGCGTCATTTTTATGGCAGACAAAAAACAAAAGCGTGATTTAATTGAATCGAACACATATCGTGAGAAAATGAACCA
CTTACAATTAGAAAAAGAATTGGTTTATAGCACATATCAGCAACTATATACATGGTTTGAAACGATTCGTAATCATAAAG
AAGTAGAACAGTTAGGGTAA

Domains

Predicted by InterproScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)

RecJ OB domain (RecJ_OB)

(455-561)

DHH family (DHH)

(82-226)

DHHA1 domain (DHHA1)

(315-439)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

52.584

99.358

0.522

Multiple sequence alignment