Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   CG474_RS17015 Genome accession   NZ_CP024120
Coordinates   3225246..3227585 (-) Length   779 a.a.
NCBI ID   WP_048724188.1    Uniprot ID   -
Organism   Bacillus cytotoxicus strain CH_1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3220246..3232585
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CG474_RS22980 (CG474_016905) - 3220287..3220463 (-) 177 WP_012095581.1 hypothetical protein -
  CG474_RS16990 (CG474_016910) - 3220550..3220741 (-) 192 WP_041810042.1 hypothetical protein -
  CG474_RS16995 (CG474_016915) - 3221010..3221699 (-) 690 WP_048724191.1 alpha/beta fold hydrolase -
  CG474_RS17000 (CG474_016920) dtd 3221835..3222275 (-) 441 WP_012095583.1 D-aminoacyl-tRNA deacylase -
  CG474_RS17005 (CG474_016925) relA 3222293..3224476 (-) 2184 WP_048724189.1 GTP diphosphokinase -
  CG474_RS17010 (CG474_016930) - 3224685..3225197 (-) 513 WP_012095585.1 adenine phosphoribosyltransferase -
  CG474_RS17015 (CG474_016935) recJ 3225246..3227585 (-) 2340 WP_048724188.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  CG474_RS17020 (CG474_016940) - 3227665..3228558 (-) 894 WP_048724186.1 cation diffusion facilitator family transporter -
  CG474_RS17025 (CG474_016945) secDF 3228840..3231098 (-) 2259 WP_048724185.1 protein translocase subunit SecDF -
  CG474_RS17030 (CG474_016950) - 3231399..3231680 (-) 282 WP_012095589.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 779 a.a.        Molecular weight: 88262.09 Da        Isoelectric Point: 4.8618

>NTDB_id=252264 CG474_RS17015 WP_048724188.1 3225246..3227585(-) (recJ) [Bacillus cytotoxicus strain CH_1]
MLQPKTRWKEKTYNEELVRELASKLQLSPLVVSLFLGRGLDTEDKIMDFFNTAEQEFHDPFLLEGMDRTVERVKQAIQNG
EQILIFGDYDADGVSSTTVLFLALQELGADVEFYIPNRFTEGYGPNEEAFRWAHQAGFSLIITVDTGIAAVYEAQVAKEL
GIDLIITDHHEPPPELPEALAIIHPKLEGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLI
VQRGLKHMRMTKNIGLKALFKVANVSQSEITEESIGFLLAPRINAVGRLEDAAPAVHLLLSEDPEEAKELAEEIDELNKL
RKDIVKQITEEAIIEVECKYPPEENKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLSIDSEKQIAKGSARSIEGFDLF
ANLSDCRDLLPHFGGHPMAAGMTLHMNDVDELRRRLNEQANAVLVEEDFIPITTIDAVCKVEDVTLSAIEDMQKLAPFGV
GNPKPCIAVKDADLESIRAIGSDGSHLKMALRDGQATLDTIGFGFGAYAKEISPVAKVSVVGEVSINEWNNFKKPQLMVQ
DIAVEAWQLFDWRSMRNVEVNLAELPKEKIIIVYFSEDVLNKFSLEGYKEQLVHASAVTRLDGEYVVLLDLPKGIEELRN
IFQVGFPARIYTLFYQENNHLFSTVPTREHFKWYYSFLHQKAPFSLRQYGEQLCRHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMAEQIQKRDLTESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHKEIEQLR

Nucleotide


Download         Length: 2340 bp        

>NTDB_id=252264 CG474_RS17015 WP_048724188.1 3225246..3227585(-) (recJ) [Bacillus cytotoxicus strain CH_1]
GTGTTACAACCGAAAACGCGTTGGAAAGAAAAAACATATAATGAAGAACTGGTGAGGGAATTAGCAAGTAAATTGCAATT
ATCACCACTTGTTGTTTCTTTATTCCTCGGTAGAGGTTTAGATACAGAAGATAAGATTATGGATTTTTTTAATACAGCAG
AGCAAGAATTTCATGATCCATTTTTATTAGAAGGCATGGATCGGACAGTAGAGCGTGTGAAACAGGCGATCCAAAATGGA
GAGCAGATTTTAATATTTGGTGACTATGATGCAGACGGAGTCAGTAGTACAACCGTACTATTTTTAGCGCTTCAAGAATT
AGGAGCAGATGTGGAATTTTATATTCCAAACCGTTTTACGGAAGGATATGGACCAAATGAAGAGGCGTTTCGTTGGGCGC
ACCAAGCCGGTTTTTCTTTAATTATTACGGTTGATACAGGAATAGCAGCAGTATATGAGGCGCAAGTGGCAAAAGAGCTT
GGAATTGATTTAATTATTACAGATCATCATGAGCCACCGCCAGAACTGCCAGAAGCACTTGCGATTATTCATCCGAAGCT
TGAGGGCGGTGTATATCCGTTTCACTATTTAGCAGGGGTTGGTGTTGCTTTTAAAGTAGCGCATGCGTTGCTAGGGCGTG
TTCCGGAGCATTTGCTAGAAATTGCTGTTATTGGTACAGTAGCTGACTTAGTATCACTTCATGGTGAGAATAGATTAATT
GTACAGCGCGGATTAAAACATATGAGAATGACAAAAAACATCGGCTTAAAAGCACTTTTTAAAGTGGCGAATGTCTCGCA
AAGTGAAATTACAGAAGAAAGTATTGGTTTTTTGTTGGCGCCTCGTATTAATGCGGTTGGACGTTTAGAAGATGCTGCTC
CGGCAGTTCATCTTCTGCTATCAGAAGATCCAGAAGAAGCAAAAGAGCTGGCAGAAGAAATTGATGAATTAAACAAACTA
CGAAAAGATATTGTGAAACAAATTACAGAGGAAGCAATTATTGAGGTGGAATGTAAATATCCACCAGAGGAGAATAAAGT
GCTTGTTCTTGCGAAAGAAGGCTGGAACCCAGGGGTAATTGGAATTGTTGCTTCTAAATTGGTAGAACGTTTTTATCGTC
CGACGATTGTGTTAAGTATTGATTCTGAGAAACAAATTGCCAAGGGATCTGCGCGCAGTATTGAAGGGTTTGATTTGTTT
GCTAATTTATCGGATTGTAGAGATTTATTACCTCATTTTGGAGGTCATCCAATGGCAGCAGGGATGACGCTTCATATGAA
TGATGTAGATGAACTGAGACGCCGTTTGAATGAGCAAGCGAATGCAGTCTTAGTAGAGGAAGATTTTATTCCGATTACAA
CTATAGATGCCGTTTGTAAAGTAGAGGATGTCACTCTTTCAGCAATTGAAGATATGCAGAAGTTGGCACCATTTGGTGTT
GGAAATCCAAAACCATGTATCGCAGTGAAAGATGCTGATTTAGAAAGTATTCGCGCAATCGGATCAGATGGCTCTCATTT
AAAAATGGCTCTTCGGGATGGACAAGCTACGCTAGATACAATTGGTTTTGGATTTGGTGCCTATGCAAAAGAGATTTCTC
CAGTTGCGAAAGTTTCTGTTGTAGGAGAAGTATCTATTAACGAATGGAATAATTTTAAGAAACCGCAGTTAATGGTGCAA
GATATCGCAGTAGAAGCGTGGCAACTATTTGATTGGCGCAGCATGCGTAATGTAGAAGTGAATTTAGCAGAACTTCCAAA
AGAAAAAATAATTATAGTGTATTTTTCTGAAGATGTATTGAATAAATTTTCTTTAGAAGGTTATAAAGAGCAACTTGTGC
ATGCATCAGCAGTCACTCGTTTAGATGGTGAGTATGTTGTTTTACTTGACTTACCAAAAGGGATAGAAGAGTTGCGAAAT
ATATTTCAAGTTGGCTTCCCGGCTCGTATTTATACGTTATTTTATCAAGAAAATAATCATTTGTTTAGCACAGTTCCAAC
GAGAGAACACTTTAAATGGTATTACTCTTTCTTACATCAAAAAGCCCCATTTTCTTTAAGGCAATATGGCGAGCAACTAT
GCCGTCATAAAGGGTGGTCAAAAGATACAGTAAATTTCATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GACGGTGTCATTTTTATGGCCGAACAAATTCAAAAGCGTGATTTAACTGAATCGAACACATATCGTGAGAAAATGAACCA
TTTACAACTTGAAAAAGAATTAGTATATAGCACATATCAACAGCTGTACACATGGTTTGAAACGATTCGTAATCATAAAG
AAATAGAACAGTTAAGATAA

Domains

Predicted by InterproScan.

RecJ OB domain (RecJ_OB)

(455-561)

DHH family (DHH)

(82-226)

DHHA1 domain (DHHA1)

(315-439)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

53.23

99.358

0.529

Multiple sequence alignment