Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   CG479_RS16640 Genome accession   NZ_CP024109
Coordinates   3166679..3169018 (-) Length   779 a.a.
NCBI ID   WP_012095586.1    Uniprot ID   A7GT91
Organism   Bacillus cytotoxicus strain CH_13     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3161679..3174018
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CG479_RS22220 (CG479_016585) - 3161720..3161896 (-) 177 WP_012095581.1 hypothetical protein -
  CG479_RS16615 (CG479_016590) - 3161983..3162174 (-) 192 WP_041810042.1 hypothetical protein -
  CG479_RS16620 (CG479_016595) - 3162443..3163132 (-) 690 WP_012095582.1 alpha/beta fold hydrolase -
  CG479_RS16625 (CG479_016600) dtd 3163268..3163708 (-) 441 WP_012095583.1 D-aminoacyl-tRNA deacylase -
  CG479_RS16630 (CG479_016605) relA 3163726..3165909 (-) 2184 WP_012095584.1 GTP diphosphokinase -
  CG479_RS16635 (CG479_016610) - 3166118..3166630 (-) 513 WP_012095585.1 adenine phosphoribosyltransferase -
  CG479_RS16640 (CG479_016615) recJ 3166679..3169018 (-) 2340 WP_012095586.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  CG479_RS16645 (CG479_016620) - 3169098..3169991 (-) 894 WP_012095587.1 cation diffusion facilitator family transporter -
  CG479_RS16650 (CG479_016625) secDF 3170273..3172531 (-) 2259 WP_012095588.1 protein translocase subunit SecDF -
  CG479_RS16655 (CG479_016630) - 3172832..3173113 (-) 282 WP_012095589.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 779 a.a.        Molecular weight: 88236.06 Da        Isoelectric Point: 4.8930

>NTDB_id=252074 CG479_RS16640 WP_012095586.1 3166679..3169018(-) (recJ) [Bacillus cytotoxicus strain CH_13]
MLQPKTRWKEKTYNEELVRELASKLQLSPLVVSLFLGRGLDTEDKIMDFFNTAEQEFHDPFLLEGMDRTVERVKQAIQNG
EQILIFGDYDADGVSSTTVLFLALQELGADVEFYIPNRFTEGYGPNEEAFRWAHQAGFSLIITVDTGIAAVHEAQVAKEL
GIDLIITDHHEPPPELPEALAIIHPKLEGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLI
VQRGLKHMRMTKNIGLKALFKVANVSQSEITEESIGFLLAPRINAVGRLEDAAPAVHLLLSEDPEEAKELAEEIDELNKL
RKDIVKQITEEAIIEVECKYPPEENKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLSIDSEKQIAKGSARSIEGFDLF
ANLSDCRDLLPHFGGHPMAAGMTLHMNDVDELRRRLNEQANAVLVEEDFIPITTIDAVCKVEDVTLSAIEDMQKLAPFGV
GNPKPCIAVKDADLESIRAIGSDGSHLKMALRDGQATLDTIGFGFGAYAKEISPVAKVSVVGEVSINEWNNFKKPQLMVQ
DIAVEAWQLFDWRSMRNVEVNLAELPKEKIIIVYFSEDVLNKFSLEGYKEQLVHASAVTRLDGEYVVLLDLPKGIEELRN
IFQVGFPARIYTLFYQENNHLFSTVPTREHFKWYYSFLHQKAPFSLRQYGEQLCRHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMAEQIQKRDLTESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHKEIEQLR

Nucleotide


Download         Length: 2340 bp        

>NTDB_id=252074 CG479_RS16640 WP_012095586.1 3166679..3169018(-) (recJ) [Bacillus cytotoxicus strain CH_13]
GTGTTACAACCGAAAACGCGTTGGAAAGAAAAAACATATAATGAAGAACTGGTGAGGGAATTAGCAAGTAAATTGCAATT
ATCACCACTTGTTGTTTCTTTATTCCTCGGTAGAGGTTTAGATACAGAAGATAAGATTATGGATTTTTTTAATACAGCAG
AGCAAGAATTTCATGATCCATTTTTATTAGAAGGCATGGATCGGACAGTAGAGCGTGTGAAACAGGCGATCCAAAATGGA
GAGCAGATTTTAATATTTGGTGACTATGATGCAGACGGAGTCAGTAGTACGACCGTACTATTTTTAGCGCTTCAAGAATT
AGGAGCAGATGTGGAATTTTATATTCCAAACCGTTTTACGGAAGGATATGGACCAAATGAAGAGGCGTTTCGTTGGGCGC
ACCAAGCCGGTTTTTCTTTAATTATTACGGTTGATACAGGAATAGCAGCAGTACATGAGGCGCAAGTGGCAAAAGAGCTT
GGAATTGATTTAATTATTACAGATCATCATGAGCCACCGCCAGAACTGCCAGAAGCACTTGCGATTATTCATCCGAAGCT
TGAGGGTGGTGTATATCCGTTTCACTATTTAGCAGGGGTTGGTGTTGCTTTTAAAGTAGCGCATGCGTTGCTAGGGCGTG
TTCCGGAGCATTTGCTAGAAATTGCTGTTATTGGTACAGTAGCTGACTTAGTATCACTTCATGGTGAGAATAGATTAATT
GTACAGCGCGGATTAAAACATATGAGAATGACAAAAAACATCGGCTTAAAAGCACTTTTTAAAGTGGCGAATGTCTCGCA
AAGTGAAATTACAGAAGAAAGTATTGGTTTTTTGTTGGCGCCTCGTATTAATGCGGTTGGACGTTTAGAAGATGCTGCTC
CGGCAGTTCATCTTCTGCTATCAGAAGATCCAGAAGAAGCAAAAGAGCTGGCAGAAGAAATTGATGAATTAAACAAACTA
CGAAAAGATATTGTGAAACAAATTACCGAGGAAGCAATTATTGAGGTGGAATGTAAATATCCACCAGAGGAGAATAAAGT
GCTTGTTCTTGCGAAAGAAGGCTGGAACCCGGGGGTAATTGGAATTGTTGCTTCTAAATTGGTAGAACGTTTTTATCGTC
CGACGATTGTGTTAAGTATTGATTCTGAGAAACAAATTGCCAAGGGATCTGCGCGCAGTATTGAAGGGTTTGATTTGTTT
GCTAATTTATCGGATTGTAGAGATTTATTACCTCATTTTGGAGGTCATCCAATGGCAGCAGGGATGACGCTTCATATGAA
TGATGTAGATGAACTGAGACGCCGTTTGAATGAACAAGCGAATGCAGTCTTAGTAGAGGAAGATTTTATTCCGATTACAA
CTATAGATGCCGTTTGTAAAGTAGAGGATGTCACTCTTTCAGCAATTGAAGATATGCAGAAGTTGGCACCATTTGGTGTT
GGAAATCCAAAACCATGTATCGCAGTGAAAGATGCTGATTTAGAAAGTATTCGCGCAATCGGATCAGATGGCTCTCATTT
AAAAATGGCTCTTCGGGATGGACAAGCTACGCTAGATACAATTGGTTTTGGATTTGGTGCCTATGCAAAAGAGATTTCTC
CAGTTGCGAAAGTTTCTGTTGTAGGAGAAGTATCTATTAACGAATGGAATAATTTTAAGAAACCGCAGTTAATGGTGCAA
GATATCGCAGTAGAAGCGTGGCAACTATTTGATTGGCGCAGCATGCGTAATGTAGAAGTGAATTTAGCAGAACTTCCAAA
AGAAAAAATAATTATAGTGTATTTTTCTGAAGATGTATTGAATAAATTTTCTTTAGAAGGTTATAAAGAGCAACTTGTGC
ATGCATCAGCAGTCACTCGTTTAGATGGTGAGTATGTTGTTTTACTTGACTTACCAAAAGGGATAGAAGAGTTGCGAAAT
ATATTTCAAGTTGGCTTCCCGGCTCGTATTTATACGTTATTTTATCAAGAAAATAATCATTTGTTTAGCACAGTTCCAAC
GAGAGAACACTTTAAATGGTATTACTCTTTCTTACATCAAAAAGCCCCATTTTCTTTAAGGCAATATGGCGAGCAACTAT
GCCGTCATAAAGGGTGGTCAAAAGATACAGTAAATTTCATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GACGGTGTCATTTTTATGGCCGAACAAATTCAAAAGCGTGATTTAACTGAATCGAACACATATCGTGAGAAAATGAACCA
TTTACAACTTGAAAAAGAATTAGTATATAGCACATATCAACAGCTGTACACATGGTTTGAAACGATTCGTAATCATAAAG
AAATAGAACAGTTAAGATAA

Domains

Predicted by InterproScan.

RecJ OB domain (RecJ_OB)

(455-561)

DHHA1 domain (DHHA1)

(315-439)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)

DHH family (DHH)

(82-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7GT91

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

53.359

99.358

0.53

Multiple sequence alignment