Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   DBX32_RS25305 Genome accession   NZ_CP028848
Coordinates   5294418..5294843 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain IMP67     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5289418..5299843
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DBX32_RS25290 (DBX32_25220) pilX 5289982..5290569 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  DBX32_RS25295 (DBX32_25225) pilY1 5290581..5294072 (+) 3492 WP_003123397.1 type 4a pilus biogenesis protein PilY1 -
  DBX32_RS25300 (DBX32_25230) pilY2 5294074..5294421 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  DBX32_RS25305 (DBX32_25235) comF 5294418..5294843 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  DBX32_RS25310 (DBX32_25240) ispH 5294890..5295834 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  DBX32_RS25315 (DBX32_25245) fkpB 5295920..5296360 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  DBX32_RS25320 (DBX32_25250) lspA 5296353..5296862 (-) 510 WP_003102615.1 signal peptidase II -
  DBX32_RS25325 (DBX32_25255) ileS 5296855..5299686 (-) 2832 WP_034020463.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=248706 DBX32_RS25305 WP_003094721.1 5294418..5294843(+) (comF) [Pseudomonas aeruginosa strain IMP67]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=248706 DBX32_RS25305 WP_003094721.1 5294418..5294843(+) (comF) [Pseudomonas aeruginosa strain IMP67]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383