Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYH   Type   Machinery gene
Locus tag   CMV25_RS03260 Genome accession   NZ_CP023392
Coordinates   670459..671394 (-) Length   311 a.a.
NCBI ID   WP_167372300.1    Uniprot ID   -
Organism   Lactococcus raffinolactis strain WiKim0068     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 665459..676394
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CMV25_RS03235 (CMV25_03235) - 665939..666382 (+) 444 WP_061775261.1 flavodoxin -
  CMV25_RS03240 (CMV25_03240) - 666501..667175 (-) 675 WP_096039557.1 hypothetical protein -
  CMV25_RS03245 (CMV25_03245) frr 667410..667967 (-) 558 WP_061774678.1 ribosome recycling factor -
  CMV25_RS03250 (CMV25_03250) pyrH 667998..668717 (-) 720 WP_061774677.1 UMP kinase -
  CMV25_RS03255 (CMV25_03255) - 668957..670150 (-) 1194 WP_096039558.1 acetate kinase -
  CMV25_RS03260 (CMV25_03260) comYH 670459..671394 (-) 936 WP_167372300.1 class I SAM-dependent methyltransferase Machinery gene
  CMV25_RS03265 (CMV25_03265) - 671556..672044 (-) 489 WP_061774674.1 GNAT family N-acetyltransferase -
  CMV25_RS03270 (CMV25_03270) murC 672145..673479 (-) 1335 WP_096039560.1 UDP-N-acetylmuramate--L-alanine ligase -
  CMV25_RS03275 (CMV25_03275) - 673748..674359 (-) 612 WP_096039561.1 hypothetical protein -

Sequence

Protein


Download         Length: 311 a.a.        Molecular weight: 34665.65 Da        Isoelectric Point: 4.4804

>NTDB_id=246590 CMV25_RS03260 WP_167372300.1 670459..671394(-) (comYH) [Lactococcus raffinolactis strain WiKim0068]
MNMEKIETAFGLLLANVQQLETRLATHFYDALIEQNVSYLGKAVSEDLQQRNEQLRALNLTKQEWQKVYQFALIKGAKDM
HLQANHQLTPDAIGYIINFMIETLSTETNLSILELGSGTGNLAETLLTSMSDKALTYTGFEVDDLMIDLSASIADVMQTS
AQFLQIDAVRPQVIEPVDLLLSDLPVGYYPDDAIAQRSVVGSQSEHTYAHHLLMAQGFKYLKADGYAIFIAPSDLLSSPQ
SDLLKKWLQDYASVAAVITLPEDIVTENHTKAIFVLQKSAQGKAPFVFPLISLTNPEIVQSFMTQFRQNMI

Nucleotide


Download         Length: 936 bp        

>NTDB_id=246590 CMV25_RS03260 WP_167372300.1 670459..671394(-) (comYH) [Lactococcus raffinolactis strain WiKim0068]
ATGAATATGGAAAAAATAGAAACGGCATTTGGCCTATTATTAGCCAACGTTCAGCAACTTGAAACACGCTTGGCAACACA
TTTTTACGATGCCTTGATTGAGCAAAATGTGAGCTATCTCGGTAAAGCTGTATCAGAAGACTTGCAGCAACGCAATGAGC
AGTTGCGTGCGCTCAATTTGACAAAACAAGAGTGGCAAAAGGTCTATCAGTTTGCCTTGATTAAGGGTGCTAAGGACATG
CACCTGCAAGCCAATCATCAGTTAACACCGGATGCAATTGGGTATATCATCAATTTCATGATTGAGACCTTATCTACCGA
AACTAACTTGTCTATTTTGGAATTAGGGTCTGGGACAGGTAATTTAGCCGAGACATTATTGACTAGCATGTCAGATAAAG
CACTAACCTATACTGGCTTTGAAGTTGATGATTTAATGATTGACCTGTCGGCTAGCATTGCCGATGTCATGCAAACTTCA
GCCCAATTTTTGCAGATTGATGCTGTGCGCCCTCAGGTTATCGAACCTGTGGATCTGTTATTGTCAGATTTACCGGTAGG
CTATTATCCAGATGATGCGATTGCGCAACGTTCAGTTGTTGGCAGTCAGAGTGAGCATACCTACGCCCATCACTTGCTGA
TGGCGCAAGGATTCAAATATCTAAAAGCAGATGGTTATGCGATTTTTATTGCACCGAGTGATTTGTTGTCTAGTCCGCAA
TCCGATTTATTAAAAAAATGGTTGCAGGATTATGCCAGCGTCGCTGCTGTGATTACTTTACCAGAAGACATTGTCACTGA
AAATCATACTAAGGCAATCTTTGTTTTACAAAAGTCTGCACAAGGTAAAGCACCCTTTGTTTTTCCTTTGATAAGTCTAA
CCAATCCTGAAATTGTGCAGTCTTTCATGACGCAATTTCGTCAGAATATGATATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYH Streptococcus mutans UA140

54.662

100

0.547
  comYH Streptococcus mutans UA159

54.662

100

0.547

Multiple sequence alignment