Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   CLI64_RS22590 Genome accession   NZ_CP023278
Coordinates   5436380..5437489 (+) Length   369 a.a.
NCBI ID   WP_103139325.1    Uniprot ID   A0A2I8AC46
Organism   Nostoc sp. CENA543     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5431380..5442489
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLI64_RS22575 (CLI64_22435) - 5432212..5433387 (+) 1176 WP_103139322.1 FIST signal transduction protein -
  CLI64_RS22580 (CLI64_22440) - 5433394..5434467 (+) 1074 WP_103139323.1 sensor histidine kinase -
  CLI64_RS22585 (CLI64_22445) - 5434885..5436183 (-) 1299 WP_103139324.1 sugar ABC transporter substrate-binding protein -
  CLI64_RS22590 (CLI64_22450) pilM 5436380..5437489 (+) 1110 WP_103139325.1 type IV pilus assembly protein PilM Machinery gene
  CLI64_RS22595 (CLI64_22455) - 5437494..5438258 (+) 765 WP_103139326.1 PilN domain-containing protein -
  CLI64_RS22600 (CLI64_22460) - 5438255..5439022 (+) 768 WP_103139327.1 pilus assembly protein PilO -
  CLI64_RS22605 (CLI64_22465) - 5439105..5439491 (+) 387 WP_103139328.1 four helix bundle protein -
  CLI64_RS22610 (CLI64_22470) - 5439575..5441938 (+) 2364 WP_103139329.1 type IV pilus secretin family protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40780.68 Da        Isoelectric Point: 4.5345

>NTDB_id=245715 CLI64_RS22590 WP_103139325.1 5436380..5437489(+) (pilM) [Nostoc sp. CENA543]
MVKSFSSLFGKSHRGVGVEIAPERVNVVQLRKQRQGLKLESLTSVPVPEGIVTDGQITDPPAMAQLIQQALAESKIKTSR
VSTGVPGRDSVVRIIPVPAELDDKELRDMVLNHEAALYLPYPREEADVDYQKLGYFVDEDGIEKVQVLLVATRKEVTDTY
ISTFEQAGLQINVLEINSFALIRTIRDRHLRELSPQEAAVLVDIEFDSTEIAIIVNGIPQFSRTVPIGTYQMQMALARAM
SLPTSRDMELLYEMTIPVTPVDADKTGVTETNPGMAGVLRILGELTDELRRSIDFYLNQSENIEVAQIFLAGPGGGLQHL
DEFFTQRLSIPTTQVDPIGSLSLQVDEQKYPAQQRSGLGIILGLGLREV

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=245715 CLI64_RS22590 WP_103139325.1 5436380..5437489(+) (pilM) [Nostoc sp. CENA543]
GTGGTGAAAAGTTTCAGTAGTCTGTTTGGCAAATCTCATAGAGGGGTAGGTGTGGAAATCGCACCAGAACGAGTCAATGT
AGTGCAACTACGCAAGCAGCGTCAAGGCTTGAAACTCGAATCTTTAACATCAGTACCAGTTCCAGAAGGAATCGTCACTG
ATGGTCAAATTACTGATCCCCCAGCTATGGCGCAACTCATTCAGCAAGCTTTAGCTGAAAGTAAAATCAAGACTTCTCGT
GTATCCACCGGCGTACCAGGACGTGATTCGGTTGTACGAATTATTCCTGTGCCGGCAGAGTTAGACGATAAAGAACTCCG
AGATATGGTGCTAAACCATGAAGCAGCCTTGTATTTACCCTATCCTCGTGAGGAAGCTGATGTAGATTATCAAAAACTTG
GGTACTTTGTAGATGAAGACGGCATCGAAAAAGTACAAGTGCTGCTAGTTGCTACCCGCAAGGAGGTGACAGATACATAT
ATTAGTACCTTTGAGCAAGCAGGATTGCAAATTAATGTTTTAGAGATTAACAGTTTTGCACTAATTCGGACAATCCGCGA
TCGCCATTTACGAGAACTCAGTCCCCAAGAAGCAGCTGTCTTAGTTGATATCGAATTTGACAGCACGGAAATAGCCATTA
TCGTCAACGGTATCCCCCAATTTTCTCGGACTGTACCCATAGGTACCTACCAAATGCAAATGGCTTTAGCTAGAGCCATG
AGTCTACCAACATCACGGGATATGGAACTTTTGTATGAAATGACGATTCCTGTCACGCCTGTAGATGCTGATAAAACAGG
AGTCACAGAAACTAATCCGGGAATGGCTGGTGTATTGCGAATCTTAGGAGAACTCACAGATGAGCTACGCCGTTCCATAG
ATTTCTACCTCAATCAAAGTGAAAACATAGAGGTAGCACAAATTTTCTTAGCAGGGCCTGGGGGCGGACTCCAACACTTA
GATGAATTCTTTACCCAAAGACTCAGCATACCTACTACCCAAGTAGATCCAATTGGGTCTTTGTCCTTACAAGTCGATGA
ACAAAAATATCCTGCTCAACAGCGTTCGGGTTTAGGAATTATCTTGGGTTTAGGACTGCGGGAGGTGTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-239)

Type IV pilus assembly protein PilM; (PilM_2)

(284-368)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I8AC46

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

64.674

99.729

0.645

Multiple sequence alignment