Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   CKF48_RS17220 Genome accession   NZ_CP022983
Coordinates   3384421..3386760 (+) Length   779 a.a.
NCBI ID   WP_095372442.1    Uniprot ID   A0A248TKZ5
Organism   Cytobacillus kochii strain BDGP4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3379421..3391760
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKF48_RS17200 (CKF48_17190) - 3380059..3380346 (+) 288 WP_233435379.1 post-transcriptional regulator -
  CKF48_RS17205 (CKF48_17195) secDF 3380578..3382845 (+) 2268 WP_095372439.1 protein translocase subunit SecDF -
  CKF48_RS17210 (CKF48_17200) - 3383117..3384016 (+) 900 WP_095372440.1 cation diffusion facilitator family transporter -
  CKF48_RS17215 (CKF48_17205) - 3384028..3384369 (+) 342 WP_095372441.1 lipopolysaccharide assembly LapA domain-containing protein -
  CKF48_RS17220 (CKF48_17210) recJ 3384421..3386760 (+) 2340 WP_095372442.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  CKF48_RS17225 (CKF48_17215) - 3386779..3387291 (+) 513 WP_095372443.1 adenine phosphoribosyltransferase -
  CKF48_RS17230 (CKF48_17220) - 3387568..3389763 (+) 2196 WP_095372444.1 RelA/SpoT family protein -
  CKF48_RS17235 (CKF48_17225) dtd 3389781..3390221 (+) 441 WP_095372445.1 D-aminoacyl-tRNA deacylase -

Sequence

Protein


Download         Length: 779 a.a.        Molecular weight: 87560.03 Da        Isoelectric Point: 4.7110

>NTDB_id=243832 CKF48_RS17220 WP_095372442.1 3384421..3386760(+) (recJ) [Cytobacillus kochii strain BDGP4]
MLHSKTRWNVRQSDEASVEELINGLNISSALATLLVNRGFNTVESARYFLFDEKKEFHDPFLLNDMDLAVSRIQRAIKNN
ESIMIFGDYDADGVTSTTVMMRTLTSLGADVQFYIPNRFTEGYGPNVPAFQYASDIGVSLIITVDTGIAAIEPAQAAKEM
GIDLIITDHHEPGPVLPECLAIIHPKLPDSRYPFKELAGVGVAFKLAHALLGNVPNDLLEFVAIGTVADLVSLTDENRLI
VKKGLKKLQSTQNLGIRSLISLANVKLAEMTEETIGFTIAPRLNAVGRLDSADPAVELLLTEDALEAEALAEEIDQMNKE
RQSIVHTMAEEAIQQVEAEYPIEENPVIVVGKEGWNPGVVGIVASRLVEKYYRPVICLSFDSQQGIAKGSARSITGFDLF
HNLSQSRDILPHFGGHPMAAGMTLKLDDVDILRQRLIQLAKEQLNEDDFIPITNLDAELRVADVNLEMIAEIQQLAPFGI
DNPKPKVMLKEANVSLLKRIGTNKTHLKITLEDNDVTLDGIGFGFGEYAEQISMDASLAIVGELSINEWNNIKKPQVFIQ
DIRIQEWQLFDIRGAKRLDTIVTHVHHETSEWIVFNKENHDKFLPYNPNVSLILSDEEAENIILDGKHVVFCDLPPQKER
LEAILRGKSPERIYCHFYQTNDIFSMATPNRDHFKWYYALIAKRGPFNLEERKAEVAKFKGWQVSYIDFMTEVFFELEFV
KIDNGMISLSDAKQKRDLSESQAFCIKQQQVKLENELLYSKAGQLKDWFDGLLQNAYVN

Nucleotide


Download         Length: 2340 bp        

>NTDB_id=243832 CKF48_RS17220 WP_095372442.1 3384421..3386760(+) (recJ) [Cytobacillus kochii strain BDGP4]
ATGTTACATTCTAAAACGCGTTGGAATGTTCGTCAATCAGATGAAGCCTCTGTAGAGGAACTCATAAACGGATTAAATAT
TTCATCTGCGTTGGCTACATTACTTGTTAATCGCGGCTTTAATACCGTCGAATCAGCACGGTATTTTTTATTTGATGAAA
AAAAAGAATTTCACGATCCTTTCTTATTAAATGATATGGATTTGGCTGTTTCGCGTATACAACGAGCGATTAAAAATAAC
GAATCGATTATGATTTTTGGAGACTATGATGCCGATGGTGTTACGAGTACTACTGTCATGATGAGAACATTAACATCACT
AGGAGCAGATGTGCAATTTTACATACCTAACCGGTTTACGGAAGGTTATGGGCCAAATGTTCCGGCATTTCAATATGCTA
GTGATATCGGTGTTTCGTTAATTATTACTGTTGATACAGGAATTGCTGCTATCGAACCTGCGCAAGCGGCTAAAGAAATG
GGAATCGATTTAATTATTACGGATCATCATGAACCAGGCCCTGTCTTGCCAGAGTGTCTGGCAATCATTCATCCGAAACT
TCCTGACAGTCGTTATCCTTTCAAAGAACTTGCTGGAGTTGGTGTAGCATTTAAGCTTGCTCATGCTCTATTAGGTAATG
TGCCAAATGATTTATTAGAGTTTGTTGCAATTGGTACAGTTGCAGACCTTGTTTCATTGACGGATGAAAATCGGTTAATT
GTGAAGAAAGGTCTTAAAAAGCTGCAATCAACACAGAATCTTGGTATCAGATCATTAATATCGTTAGCTAATGTCAAATT
AGCAGAGATGACAGAAGAGACAATCGGCTTTACGATTGCACCAAGATTAAATGCTGTAGGTAGATTAGATTCTGCGGACC
CTGCAGTGGAATTGTTATTAACTGAAGATGCGCTTGAGGCTGAAGCGTTAGCTGAAGAAATCGACCAAATGAATAAAGAG
CGACAATCAATTGTTCATACCATGGCTGAAGAAGCGATTCAACAAGTTGAGGCGGAATATCCAATTGAGGAAAATCCGGT
CATTGTAGTAGGTAAAGAAGGTTGGAACCCTGGGGTTGTTGGAATTGTGGCTTCAAGGCTAGTAGAGAAATATTATCGTC
CTGTTATATGTCTAAGTTTCGATTCACAACAAGGAATAGCTAAAGGATCTGCGAGGAGTATAACTGGCTTTGATCTGTTT
CATAATCTGTCGCAATCTCGAGATATCCTTCCTCATTTTGGTGGTCACCCAATGGCTGCAGGAATGACCTTGAAACTTGA
TGATGTTGACATACTTCGTCAAAGGCTTATCCAGCTTGCGAAGGAACAGTTAAACGAAGATGACTTTATACCGATTACTA
ATCTAGATGCTGAATTAAGGGTAGCTGATGTTAATTTAGAAATGATCGCTGAAATACAACAATTGGCACCGTTTGGTATT
GATAATCCTAAGCCGAAAGTAATGTTAAAAGAGGCGAATGTCAGTCTGTTAAAGAGAATTGGGACCAATAAAACACATCT
GAAGATTACATTAGAAGATAATGATGTGACATTGGATGGAATTGGATTTGGATTTGGAGAATACGCAGAACAAATTTCTA
TGGATGCTAGTCTTGCTATTGTAGGGGAACTTTCTATTAACGAATGGAATAATATAAAGAAGCCACAAGTCTTTATCCAA
GATATACGTATACAAGAATGGCAATTATTTGATATTAGAGGCGCCAAACGTTTAGACACAATTGTCACACATGTCCATCA
TGAAACCTCTGAATGGATTGTATTTAATAAGGAAAATCATGATAAATTTTTACCTTATAATCCCAATGTATCGTTGATTC
TGTCTGACGAAGAGGCAGAAAATATTATATTAGATGGTAAGCATGTGGTTTTTTGTGATTTACCACCACAAAAGGAAAGA
CTAGAGGCTATTTTGCGTGGGAAAAGTCCAGAAAGAATTTATTGTCATTTCTATCAAACAAATGATATATTCTCTATGGC
GACTCCAAACCGTGACCATTTTAAATGGTACTATGCGCTCATTGCTAAGAGAGGGCCTTTTAACCTTGAAGAAAGAAAAG
CAGAGGTAGCTAAATTTAAAGGCTGGCAAGTTTCTTATATTGATTTTATGACTGAAGTGTTTTTTGAACTAGAATTTGTT
AAAATAGATAATGGGATGATTTCTCTTAGTGATGCAAAACAAAAGAGAGATTTATCGGAATCACAGGCTTTTTGCATAAA
GCAACAACAAGTGAAGTTAGAAAATGAATTGCTTTATTCAAAAGCAGGTCAATTAAAAGATTGGTTTGATGGGCTGCTTC
AGAATGCCTATGTGAATTGA

Domains

Predicted by InterproScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-769)

RecJ OB domain (RecJ_OB)

(456-561)

DHH family (DHH)

(83-226)

DHHA1 domain (DHHA1)

(315-439)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A248TKZ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

53.085

99.872

0.53

Multiple sequence alignment