Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   CIB87_RS21670 Genome accession   NZ_CP022674
Coordinates   4180614..4182956 (-) Length   780 a.a.
NCBI ID   WP_114896814.1    Uniprot ID   A0AA86LXP0
Organism   Priestia megaterium strain SR7     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4175614..4187956
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CIB87_RS21645 (CIB87_21580) - 4176007..4176183 (-) 177 WP_035335109.1 hypothetical protein -
  CIB87_RS21650 (CIB87_21585) - 4176350..4177234 (+) 885 WP_310883615.1 N-acetylmuramoyl-L-alanine amidase -
  CIB87_RS21655 (CIB87_21590) dtd 4177225..4177686 (-) 462 WP_114896813.1 D-aminoacyl-tRNA deacylase -
  CIB87_RS21660 (CIB87_21595) - 4177702..4179900 (-) 2199 WP_071270497.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  CIB87_RS21665 (CIB87_21600) - 4180086..4180598 (-) 513 WP_013059309.1 adenine phosphoribosyltransferase -
  CIB87_RS21670 (CIB87_21605) recJ 4180614..4182956 (-) 2343 WP_114896814.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  CIB87_RS21675 (CIB87_21610) secDF 4183099..4185336 (-) 2238 WP_114896815.1 protein translocase subunit SecDF -
  CIB87_RS21680 (CIB87_21615) - 4185474..4185767 (-) 294 WP_028411692.1 post-transcriptional regulator -
  CIB87_RS21685 (CIB87_21620) spoVB 4185832..4187442 (+) 1611 WP_114896816.1 stage V sporulation protein B -

Sequence

Protein


Download         Length: 780 a.a.        Molecular weight: 87915.46 Da        Isoelectric Point: 4.6573

>NTDB_id=241513 CIB87_RS21670 WP_114896814.1 4180614..4182956(-) (recJ) [Priestia megaterium strain SR7]
MLQSKKRWNVQECDEQLVNEFVENLNITPLVASLLLNRGMTTVEAAREFLHVDAQTFHDPFLLHDMDKAIARIQQAIDHN
EKIMIYGDYDADGVSSTSVMLSALQQLGADTDFYIPNRFTEGYGPNKAAFDKINAGGYQLVITVDTGISAVEETAYAREL
DFDLIITDHHEPGPVLPDAYAIIHPKLPGSTYPFKELAGVGVAFKLAHALLGELPEHLLELAVIGTIADLVPLVDENRLI
ARKGLDYLKLTKRTGIEALLSVCGVKREEITEDTIGFAMAPRINAVGRLQDADPAVHLLMTTNAEEAKGLAKEIDALNKE
RQQIVNDITEEAVEFVENMYPPDENSVLIVEGEGWNAGVVGIVASRLVEKFYRPTIVLSIDPENRIAKGSARSIEGFDLF
ANLSKCRDILPHFGGHPMAAGMTLNSDDVEELRTRLNEQASNVLTERDFIPITSVDTVCTTEEITLENIEQMSALAPFGM
HNPKPKVLIKDVHTTSMRKIGANKNHLKLVFEDSGVSVDAVGFGLGYILDEVSPIAKVSLVGELSINEWNNMRKPQLMVE
DISVDEWQMFDYRSAVDVKRFFEQVFTDQMKVVAFHDSTIDKYKSLLPMEHVVLIQNEEQAKACMFAGEQIVLLDLPPLQ
EWLEALFVQGIPSRIYAIFLTEEDHYFDTIPTRDHFKWFYGFLAKKGPFDLKRYKEDLAIHKGWSKETIDFMAKVFFELE
FVTINNGLISLVKNSAKRDLIESATYRQKQQQIEIEKNFIYTSYVQLKNQFQDIFKRSIE

Nucleotide


Download         Length: 2343 bp        

>NTDB_id=241513 CIB87_RS21670 WP_114896814.1 4180614..4182956(-) (recJ) [Priestia megaterium strain SR7]
ATGTTACAGTCAAAAAAACGTTGGAATGTTCAAGAATGTGATGAACAACTTGTTAATGAATTTGTAGAAAACCTCAATAT
TACACCTCTAGTAGCTTCGTTATTACTTAATAGAGGCATGACAACAGTAGAAGCGGCACGTGAGTTTTTACACGTTGATG
CACAAACGTTCCACGATCCGTTTTTATTGCACGATATGGACAAAGCGATAGCTCGTATTCAGCAAGCAATTGATCATAAT
GAAAAAATTATGATCTATGGAGATTACGATGCGGACGGCGTCAGCAGTACATCTGTTATGTTATCTGCTTTACAGCAGCT
CGGTGCTGATACTGATTTTTATATTCCTAATCGCTTTACAGAAGGTTATGGGCCGAATAAGGCAGCATTTGATAAAATAA
ACGCAGGCGGCTATCAGCTGGTGATTACGGTAGACACAGGTATTTCGGCAGTCGAAGAAACGGCGTATGCACGTGAATTA
GATTTTGATTTAATTATTACGGATCACCACGAGCCGGGTCCGGTTTTACCGGATGCGTATGCAATTATTCATCCAAAGCT
GCCGGGAAGTACATATCCATTTAAAGAACTGGCAGGTGTAGGAGTGGCTTTTAAACTTGCTCATGCGCTGTTAGGGGAGC
TTCCGGAACATTTGTTAGAACTAGCAGTGATTGGGACGATTGCTGACTTAGTTCCTTTAGTTGACGAAAACCGCTTAATT
GCTCGAAAAGGTCTTGACTATCTTAAACTCACGAAGCGCACTGGAATTGAAGCGCTGCTGAGCGTATGTGGAGTAAAACG
CGAAGAAATTACTGAAGATACAATTGGATTTGCTATGGCTCCTCGCATTAATGCAGTAGGACGTCTTCAAGATGCTGATC
CAGCTGTTCACTTGCTTATGACAACCAATGCTGAAGAAGCAAAAGGTTTAGCTAAAGAAATTGATGCGTTAAACAAGGAA
AGGCAGCAAATCGTAAACGATATTACGGAAGAAGCCGTTGAATTTGTTGAAAATATGTATCCTCCGGATGAAAACAGCGT
TCTTATTGTAGAGGGAGAAGGATGGAATGCAGGCGTTGTTGGAATTGTCGCTTCTAGGCTCGTAGAAAAGTTCTACAGAC
CAACAATTGTACTAAGTATTGACCCTGAAAACCGTATTGCCAAAGGTTCAGCAAGAAGTATCGAAGGCTTTGACCTATTC
GCGAACTTATCTAAATGCCGTGACATTTTGCCGCATTTTGGAGGACATCCAATGGCAGCCGGCATGACGTTAAACAGCGA
TGATGTAGAAGAGCTGCGCACACGTCTTAATGAACAAGCAAGCAACGTGTTAACCGAACGGGATTTTATTCCAATTACAT
CAGTTGATACAGTATGTACAACAGAGGAAATTACGCTGGAGAACATTGAACAAATGAGTGCGCTTGCTCCATTTGGTATG
CATAACCCGAAGCCAAAAGTTCTCATTAAAGATGTTCATACAACATCTATGCGGAAAATTGGTGCTAATAAAAATCATTT
AAAACTCGTATTTGAAGACAGCGGTGTATCGGTTGATGCAGTAGGATTTGGGTTAGGCTATATATTAGATGAAGTATCTC
CTATAGCTAAGGTATCATTAGTAGGTGAACTATCTATTAATGAGTGGAACAATATGCGCAAACCGCAGCTGATGGTAGAA
GATATCTCTGTAGACGAGTGGCAGATGTTTGATTATCGCAGTGCGGTTGATGTCAAACGTTTCTTTGAGCAGGTATTCAC
GGATCAAATGAAAGTTGTTGCTTTTCACGATTCAACAATTGATAAATACAAGTCGCTGCTGCCGATGGAGCATGTTGTTC
TTATTCAAAATGAAGAACAGGCAAAAGCATGTATGTTTGCAGGGGAACAGATCGTTTTATTAGATTTGCCTCCTTTACAG
GAGTGGCTTGAAGCTTTGTTTGTCCAAGGTATTCCTTCTAGAATATACGCTATTTTCTTAACGGAAGAAGACCATTATTT
TGATACAATCCCAACTCGCGATCACTTTAAATGGTTTTACGGCTTTTTAGCGAAAAAGGGTCCTTTTGATTTAAAACGCT
ACAAAGAAGATTTAGCGATTCATAAGGGCTGGTCGAAAGAAACGATTGATTTTATGGCAAAGGTGTTTTTTGAGTTAGAA
TTTGTTACAATAAACAATGGCTTAATTTCGTTAGTGAAAAATTCTGCGAAACGTGATTTAATAGAATCTGCGACTTATCG
CCAAAAGCAGCAGCAGATTGAAATAGAAAAAAACTTTATTTATACGTCATATGTACAGCTTAAAAATCAATTTCAAGATA
TTTTTAAGCGTTCGATTGAATGA

Domains

Predicted by InterproScan.

DHH family (DHH)

(82-226)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-770)

RecJ OB domain (RecJ_OB)

(455-561)

DHHA1 domain (DHHA1)

(306-439)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

55.385

100

0.554

Multiple sequence alignment