Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   CEQ48_RS05865 Genome accession   NZ_CP022353
Coordinates   83940..85655 (+) Length   571 a.a.
NCBI ID   WP_181710721.1    Uniprot ID   -
Organism   Vibrio tarriae strain 2521-89     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 78940..90655
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CEQ48_RS05845 (CEQ48_05845) pilM 81233..82249 (+) 1017 WP_089070616.1 type IV pilus assembly protein PilM Machinery gene
  CEQ48_RS05850 (CEQ48_05850) pilN 82239..82823 (+) 585 WP_181710719.1 PilN domain-containing protein Machinery gene
  CEQ48_RS05855 (CEQ48_05855) pilO 82816..83406 (+) 591 WP_181710720.1 type 4a pilus biogenesis protein PilO Machinery gene
  CEQ48_RS05860 (CEQ48_05860) pilP 83396..83914 (+) 519 WP_089070619.1 pilus assembly protein PilP Machinery gene
  CEQ48_RS05865 (CEQ48_05865) pilQ 83940..85655 (+) 1716 WP_181710721.1 type IV pilus secretin PilQ Machinery gene
  CEQ48_RS05870 (CEQ48_05870) aroK 85842..86366 (+) 525 WP_000818616.1 shikimate kinase AroK -
  CEQ48_RS05875 (CEQ48_05875) aroB 86394..87479 (+) 1086 WP_198301217.1 3-dehydroquinate synthase -
  CEQ48_RS05880 (CEQ48_05880) - 87534..89009 (+) 1476 WP_089070622.1 AAA family ATPase -
  CEQ48_RS05885 (CEQ48_05885) - 89079..89912 (+) 834 WP_089070623.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62402.47 Da        Isoelectric Point: 4.6094

>NTDB_id=238619 CEQ48_RS05865 WP_181710721.1 83940..85655(+) (pilQ) [Vibrio tarriae strain 2521-89]
MKNGLKTYVAQTWLTLWVGLALCASSMAFSAESASANQLENIDFRVNKDKAAVLIVELASPSAVVDVQKVQEGLNIELLK
TDVSDDKLYLLDVKDFSTAVQSVEVFRKEPSTQLVVTVDGEFKHDYTLKGKYLEVVISKLKADEKPKPKSILEKEGKLIS
INFQDIPVRNVLQLIADYNGFNLVVSDSVAGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILIAPKEELDLREKQEL
EKARLAEELGDLKSEIIKINFAKASDIAAMIGGEGNVNMLSERGSISIDERTNSLLIRELPENIAVIREIIESLDIPVKQ
VQIEARIVTVKEGNLDELGVRWGVMSTNGSHSVGGTIESNLWQQGLLADDEFPVDEFLNVNLASTSANASSIAFQVAKLG
SGTLLDLELSALQNESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGATTVSFKKAVLSLKVTPQITPDNRLVLDL
SVTQDRRGETVKTGTGEAVSIDTQRIGTQVLVNNGETVVLGGIFQHSINNSVDKIPLLGDLPVLGALFRRTYEQMGKSEL
LIFVTPKVVIQ

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=238619 CEQ48_RS05865 WP_181710721.1 83940..85655(+) (pilQ) [Vibrio tarriae strain 2521-89]
ATGAAAAATGGATTGAAAACTTATGTCGCACAGACTTGGCTAACCTTGTGGGTTGGTTTGGCACTTTGTGCTTCTTCCAT
GGCGTTTAGCGCTGAATCAGCCTCCGCCAACCAGTTGGAAAACATCGACTTTCGGGTCAATAAAGATAAGGCGGCCGTAT
TAATTGTCGAATTGGCCTCTCCATCGGCGGTGGTAGATGTACAAAAAGTCCAAGAAGGGTTGAACATTGAGCTACTTAAA
ACCGATGTGTCGGATGACAAACTCTACCTTTTGGATGTGAAAGATTTCTCCACCGCAGTGCAGAGTGTCGAAGTATTTCG
AAAAGAACCGAGTACACAACTTGTAGTGACGGTTGATGGTGAGTTTAAGCACGATTACACCTTAAAGGGAAAATATCTTG
AGGTGGTGATCAGCAAACTGAAAGCCGATGAAAAGCCTAAGCCAAAGAGTATCTTGGAAAAAGAAGGCAAGCTTATCTCG
ATCAACTTCCAAGACATTCCAGTACGTAATGTGTTGCAGTTGATTGCCGACTATAACGGCTTCAACTTAGTAGTGTCGGA
CTCTGTTGCTGGAAATCTGACCTTGCGCTTGGATGGCGTTCCTTGGCAACAAGTGTTAGATATTATTCTGCAAGTCAAAG
GGTTGGATAAGCGGGTAGATGGCAATGTTATTTTGATTGCGCCGAAAGAGGAGCTCGATTTACGCGAAAAACAAGAGCTC
GAAAAAGCCCGCTTAGCGGAAGAACTGGGTGATTTAAAATCGGAAATCATCAAGATTAACTTTGCCAAAGCGTCTGACAT
TGCGGCGATGATCGGCGGTGAAGGTAACGTCAACATGTTGTCTGAACGAGGTTCGATCAGCATCGATGAACGGACGAACT
CACTGTTGATTCGTGAATTGCCAGAAAATATCGCAGTGATCCGCGAGATCATTGAATCACTGGATATACCAGTTAAACAA
GTGCAAATCGAAGCGCGGATTGTGACCGTAAAAGAAGGTAACCTTGATGAGCTTGGTGTACGTTGGGGCGTGATGTCGAC
CAATGGTAGCCATTCAGTAGGGGGAACCATTGAAAGTAACTTATGGCAACAAGGGTTATTGGCGGATGATGAATTTCCGG
TTGATGAATTCTTAAATGTGAACTTGGCTTCAACCTCTGCAAATGCGTCTTCAATTGCTTTCCAAGTGGCTAAGTTAGGC
TCTGGTACTTTATTAGATCTGGAGCTTTCAGCATTACAAAATGAATCGAAGGCAGAAATCATCTCTAGCCCTCGTTTGAT
CACTACCAACAAACAGCCAGCCTATATTGAGCAAGGTACCGAGATCCCTTATCTGGAATCCTCTTCAAGCGGCGCAACAA
CTGTATCGTTTAAAAAAGCAGTGCTGAGTTTAAAAGTGACGCCACAAATCACGCCAGATAACCGCTTGGTGCTTGATTTG
AGCGTGACCCAAGACCGCCGCGGCGAAACAGTGAAAACGGGCACTGGAGAGGCTGTCTCTATCGATACTCAGCGAATTGG
TACTCAAGTGTTGGTGAATAACGGGGAAACCGTAGTGCTAGGTGGTATTTTCCAACATAGCATCAATAACTCGGTAGATA
AGATCCCATTGTTGGGTGACCTGCCTGTTTTGGGCGCGTTATTTAGACGAACCTATGAGCAAATGGGTAAAAGTGAACTG
CTGATTTTCGTCACGCCGAAAGTAGTTATTCAATAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(255-320)

Secretin and TonB N terminus short domain (STN)

(181-229)

Bacterial type II and III secretion system protein (Secretin)

(411-569)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

96.848

100

0.968
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

96.848

100

0.968
  pilQ Vibrio campbellii strain DS40M4

78.727

96.322

0.758
  pilQ Legionella pneumophila strain ERS1305867

42.989

94.921

0.408
  pilQ Legionella pneumophila strain Lp02

42.989

94.921

0.408
  comQ Acinetobacter baylyi ADP1

39.161

100

0.392
  pilQ Acinetobacter baumannii D1279779

37.649

100

0.387
  pilQ Acinetobacter baumannii strain A118

37.649

100

0.387
  pilQ Pseudomonas aeruginosa PAK

39.568

97.373

0.385

Multiple sequence alignment