Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   I3O_RS21240 Genome accession   NZ_CP028117
Coordinates   3953368..3953835 (+) Length   155 a.a.
NCBI ID   WP_000339836.1    Uniprot ID   -
Organism   Escherichia coli O111 str. RM9322     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3948368..3958835
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I3O_RS21190 (I3O_21185) rplP 3948478..3948888 (-) 411 WP_000941212.1 50S ribosomal protein L16 -
  I3O_RS21195 (I3O_21190) rpsC 3948901..3949602 (-) 702 WP_000529945.1 30S ribosomal protein S3 -
  I3O_RS21200 (I3O_21195) rplV 3949620..3949952 (-) 333 WP_000447529.1 50S ribosomal protein L22 -
  I3O_RS21205 (I3O_21200) rpsS 3949967..3950245 (-) 279 WP_001138117.1 30S ribosomal protein S19 -
  I3O_RS21210 (I3O_21205) rplB 3950262..3951083 (-) 822 WP_000301864.1 50S ribosomal protein L2 -
  I3O_RS21215 (I3O_21210) rplW 3951101..3951403 (-) 303 WP_000617544.1 50S ribosomal protein L23 -
  I3O_RS21220 (I3O_21215) rplD 3951400..3952005 (-) 606 WP_000424395.1 50S ribosomal protein L4 -
  I3O_RS21225 (I3O_21220) rplC 3952016..3952645 (-) 630 WP_000579833.1 50S ribosomal protein L3 -
  I3O_RS21230 (I3O_21225) rpsJ 3952678..3952989 (-) 312 WP_001181004.1 30S ribosomal protein S10 -
  I3O_RS21240 (I3O_21235) pilD 3953368..3953835 (+) 468 WP_000339836.1 A24 family peptidase Machinery gene
  I3O_RS21245 (I3O_21240) bfr 3953832..3954308 (-) 477 WP_000675515.1 bacterioferritin -
  I3O_RS21250 (I3O_21245) bfd 3954381..3954575 (-) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  I3O_RS21260 (I3O_21255) tuf 3954758..3955942 (-) 1185 WP_000031783.1 elongation factor Tu -
  I3O_RS21265 (I3O_21260) fusA 3956013..3958127 (-) 2115 WP_000124700.1 elongation factor G -
  I3O_RS21270 (I3O_21265) rpsG 3958224..3958694 (-) 471 WP_001138043.1 30S ribosomal protein S7 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16951.08 Da        Isoelectric Point: 8.1296

>NTDB_id=237919 I3O_RS21240 WP_000339836.1 3953368..3953835(+) (pilD) [Escherichia coli O111 str. RM9322]
MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=237919 I3O_RS21240 WP_000339836.1 3953368..3953835(+) (pilD) [Escherichia coli O111 str. RM9322]
ATGGACGCTAACCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTAGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361

Multiple sequence alignment