Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   C6950_RS16485 Genome accession   NZ_CP027550
Coordinates   3117777..3118541 (+) Length   254 a.a.
NCBI ID   WP_001136233.1    Uniprot ID   Q8FCN0
Organism   Escherichia coli strain 2015C-4136CT1     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 3112777..3123541
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6950_RS16465 acpT 3113731..3114318 (+) 588 WP_000285789.1 4'-phosphopantetheinyl transferase AcpT -
  C6950_RS16470 nikA 3114429..3116003 (+) 1575 WP_000493122.1 nickel ABC transporter substrate-binding protein -
  C6950_RS16475 nikB 3116003..3116947 (+) 945 WP_000947070.1 nickel ABC transporter permease subunit NikB -
  C6950_RS16480 nikC 3116944..3117777 (+) 834 WP_001008954.1 nickel ABC transporter permease subunit NikC -
  C6950_RS16485 amiE 3117777..3118541 (+) 765 WP_001136233.1 nickel import ATP-binding protein NikD Regulator
  C6950_RS16490 nikE 3118538..3119344 (+) 807 WP_001357967.1 nickel import ATP-binding protein NikE -
  C6950_RS16495 nikR 3119350..3119751 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  C6950_RS16500 - 3119950..3120696 (+) 747 WP_001296489.1 UTRA domain-containing protein -
  C6950_RS16505 - 3120721..3121194 (+) 474 WP_001357966.1 PTS sugar transporter subunit IIA -
  C6950_RS16510 - 3121191..3121472 (+) 282 WP_000084021.1 PTS sugar transporter subunit IIB -
  C6950_RS16515 - 3121549..3122907 (+) 1359 WP_001306345.1 PTS galactitol transporter subunit IIC -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26840.46 Da        Isoelectric Point: 6.6882

>NTDB_id=235588 C6950_RS16485 WP_001136233.1 3117777..3118541(+) (amiE) [Escherichia coli strain 2015C-4136CT1]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNVPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=235588 C6950_RS16485 WP_001136233.1 3117777..3118541(+) (amiE) [Escherichia coli strain 2015C-4136CT1]
ATGCCACAACAGATTGAACTCCGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTGCA
ACGTGGGCGTGTGCTGGCGTTAGTCGGCGGTAGTGGCAGCGGGAAGTCGCTGACCTGCGCCGCGACGCTGGGCATTCTGC
CTGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTGGCCGATGGAAAACCGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGTGCCTTTAATCCGCTGCACACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGACGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTCTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGCGAATCACCG
TTTATCATCGCCGATGAACCAACCACCGATCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTTACCCATGATATGGGCGTGGTAGCGCGTCTGGCAGACGATGTGGCGGTAA
TGTCTCACGGTAAGATTGTTGAACAGGGCGATGTAGAAACGCTGTTTAACGTCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8FCN0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398

Multiple sequence alignment