Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   S101359_RS05680 Genome accession   NZ_CP021500
Coordinates   1173858..1177358 (+) Length   1166 a.a.
NCBI ID   WP_088116879.1    Uniprot ID   -
Organism   Bacillus atrophaeus strain SRCM101359     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1168858..1182358
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  S101359_RS05655 (S101359_01119) - 1169252..1170745 (+) 1494 WP_088116876.1 monooxygenase -
  S101359_RS05660 (S101359_01120) - 1170829..1171359 (+) 531 WP_088116877.1 MarR family transcriptional regulator -
  S101359_RS05665 (S101359_01121) - 1171411..1172559 (-) 1149 WP_010788164.1 AbrB family transcriptional regulator -
  S101359_RS05670 - 1172793..1173119 (-) 327 WP_088116878.1 four-helix bundle copper-binding protein -
  S101359_RS05675 (S101359_01122) - 1173215..1173652 (-) 438 WP_003327683.1 ferritin family protein -
  S101359_RS05680 (S101359_01123) addB 1173858..1177358 (+) 3501 WP_088116879.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  S101359_RS05685 (S101359_01124) addA 1177345..1181049 (+) 3705 WP_088116880.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  S101359_RS05690 (S101359_01125) sbcD 1181120..1182298 (+) 1179 WP_088116881.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134378.22 Da        Isoelectric Point: 5.1681

>NTDB_id=231272 S101359_RS05680 WP_088116879.1 1173858..1177358(+) (addB) [Bacillus atrophaeus strain SRCM101359]
MGAEFLVGRSGSGKTKLIIDSIQDELRRNPFGKPIIFLVPDQMTFLMEYELAKTPGIGGMIRAQVFSFSRLAWRVLQHTG
GMNRPFVTSTGIQMLLRKLIEEHKQEFKVYQKASDKSGFTEQVERMLTEFKRYCLEPEDIRRMIESGTATDYRGERVLTD
KLHDLYILYQQMEKSLENQYLHSEDYLTLLAEQIPYAEDLKGAHVYVDGFYQFTPQEFRVLTQLMAQAEHVTFSLTADKP
SHVQAPDELDLFRMTGKTYYQLHQAASELHLDITNRELTETKRYQQTPELAHLEAMYDARPAVPFLEKQEAFTVMQAANR
RTELEGIAREIHALVRDKGLRYKDMAILARQPEDYKDMVKEVFADYEIPYFIDGKASMLNHPLVEFIRSSLDVIKGNWRY
EAVFRCVKTELLFPLDRPKNIIREQVDQLENYCIAYGIKGDRWTRGDRFVYRRFVSLDDDFAQTDQEIEMENMLNDTRDW
IVPPLFRLQKRMKQAKTVQDMAEALYLYLEETDIPLKLDQERQRAEEDGRIIEAQQHNQAWDAVIQLLEEFVEMMGEDEI
SLSLFQQMMETGAESLKFALIPPALDQVFVGNMDLSRMYGTRCTFVIGANDGVLPARPEDDGVLSDDDREWLKTIGVELS
SGGRERLLDEHFLIYMALSSPSDRLYVSYPIADAEGKTLLPSTVVKRLTELFPEHQERLLTNEPEQVSDCEQLLYLVNKS
VAQSYTASQFRLWLREYDISDVWWSAYNVLMNEPDRLNSKKLFSSLFFRNEVKPLERAVSRQLYGEHIHGSVSRMETFNA
CPFSHFASHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKLDWRDLTKDQCNKFSYEAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVARVSGILSEHAKASGFAPVGLELGFGGKGPLPPLTFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLSEVYYGLALQMLTYLDLSITYSADWLGMKATPAGVLYFHIHDPMIQASVPLGLDEIEQEIFKKFKMKGL
LLGDQEAIRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEEEFHLLTQHVRRTFQEAGEQITDGCVSIEPYKLKDKTP
CTYCSFKSVCQFDESLEENEYRTLKAEKDHTILDWLKKEGDGDANS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=231272 S101359_RS05680 WP_088116879.1 1173858..1177358(+) (addB) [Bacillus atrophaeus strain SRCM101359]
TTGGGAGCAGAGTTTTTAGTAGGCAGATCCGGGAGTGGAAAAACGAAGCTGATCATAGACAGCATTCAAGATGAGCTGCG
CCGGAATCCATTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATATGAGCTTGCTAAAA
CGCCTGGTATAGGCGGAATGATACGCGCTCAAGTGTTCAGCTTTTCACGTTTGGCCTGGCGTGTCCTGCAGCATACGGGC
GGAATGAACCGGCCGTTTGTCACCAGCACGGGAATCCAAATGCTTTTGAGAAAGCTCATTGAAGAGCACAAACAGGAGTT
TAAAGTCTATCAAAAAGCGAGTGACAAAAGCGGGTTTACCGAACAGGTAGAACGGATGCTGACGGAATTTAAACGGTACT
GCCTTGAGCCTGAAGATATCCGCCGCATGATTGAAAGCGGAACTGCCACAGACTATCGCGGCGAGCGGGTGTTAACCGAT
AAGCTGCATGATTTGTATATTTTATATCAGCAGATGGAAAAGAGCCTTGAAAACCAGTATCTTCATTCAGAGGATTATTT
AACGCTGCTGGCGGAACAGATCCCGTATGCGGAAGATTTGAAAGGGGCGCATGTCTATGTAGACGGCTTTTATCAGTTTA
CTCCGCAGGAATTCAGAGTGCTTACGCAGCTTATGGCACAGGCTGAGCATGTGACGTTTTCACTGACGGCGGACAAGCCG
TCACATGTGCAGGCGCCTGATGAGCTGGATCTGTTCAGAATGACAGGCAAAACATATTATCAGCTCCATCAAGCAGCTTC
GGAGCTTCATTTGGACATCACAAACAGAGAACTGACCGAGACGAAACGATATCAGCAAACACCGGAGCTTGCCCATTTAG
AAGCAATGTATGACGCGCGCCCGGCTGTTCCGTTTTTGGAAAAGCAAGAAGCCTTTACCGTGATGCAGGCTGCGAACAGG
CGGACAGAGCTGGAAGGAATTGCCCGTGAAATTCATGCTCTTGTAAGAGATAAGGGATTACGCTACAAGGATATGGCAAT
TCTCGCCCGCCAGCCGGAAGATTATAAGGATATGGTCAAAGAAGTATTTGCAGATTATGAAATTCCTTACTTTATAGATG
GAAAGGCATCCATGCTGAACCATCCGCTTGTTGAGTTTATCCGTTCAAGCCTTGATGTCATAAAGGGAAATTGGAGATAT
GAAGCGGTGTTTCGCTGTGTGAAAACCGAACTGTTATTCCCGCTTGACCGTCCGAAGAACATCATAAGGGAGCAGGTCGA
TCAACTGGAAAACTATTGTATCGCTTACGGAATTAAAGGCGACCGATGGACGAGAGGCGACCGTTTTGTTTACAGGCGGT
TTGTTTCACTGGATGATGATTTTGCGCAGACAGATCAAGAGATTGAAATGGAAAACATGCTGAATGACACAAGAGACTGG
ATTGTTCCGCCGCTTTTCCGTCTTCAAAAACGGATGAAACAAGCAAAAACGGTCCAAGACATGGCGGAGGCTCTTTATCT
TTATTTAGAAGAAACGGATATTCCGTTAAAGCTGGACCAAGAAAGACAGCGCGCGGAAGAGGACGGGAGAATTATCGAAG
CGCAGCAGCATAATCAGGCTTGGGACGCTGTCATACAGCTTCTCGAGGAATTTGTGGAAATGATGGGCGAAGACGAAATT
TCTCTGTCTCTTTTTCAGCAAATGATGGAGACGGGTGCGGAATCGTTAAAATTCGCTCTGATTCCGCCTGCACTCGATCA
AGTCTTTGTCGGCAATATGGATTTATCGAGAATGTACGGTACACGCTGCACCTTTGTTATCGGTGCGAACGACGGCGTAC
TTCCGGCGCGGCCTGAGGATGACGGGGTGCTGTCTGATGACGACCGGGAATGGCTGAAAACCATTGGAGTCGAGCTGTCA
TCCGGCGGGCGCGAACGTCTGCTTGATGAGCACTTTCTCATATATATGGCGCTTTCCAGTCCGTCTGACCGCCTGTATGT
ATCCTACCCGATCGCAGACGCCGAAGGTAAAACGCTTTTGCCGTCAACTGTGGTCAAACGGCTTACAGAACTGTTTCCGG
AGCATCAGGAAAGGCTTTTGACCAATGAACCGGAACAGGTCAGCGACTGCGAACAGCTCTTGTATCTCGTCAATAAAAGC
GTGGCGCAATCGTATACGGCAAGCCAATTCAGGTTGTGGTTAAGGGAGTATGACATCAGTGATGTATGGTGGAGCGCGTA
TAATGTATTGATGAATGAACCGGACAGGCTGAACTCGAAAAAACTTTTCTCCAGCCTGTTTTTCAGAAATGAAGTCAAAC
CGCTTGAACGCGCTGTATCGAGACAGCTGTACGGCGAGCATATTCATGGAAGTGTGTCCAGAATGGAGACCTTTAACGCT
TGTCCGTTCTCCCATTTTGCGTCCCACGGCTTACATCTGAAAGAACGGCAATTTTTCAAGCTGGAAGCGCCGGATATCGG
ACAGCTGTTCCATTCCAGTCTGAAGCTGATTTCAGACCGGCTTCGTGAACAAAAGCTGGATTGGCGCGATTTAACAAAAG
ACCAATGTAATAAGTTCTCTTACGAGGCGGTGGAGCGGCTCGCGCCGAAGCTCCAAAAAGAAATTCTGCTTAGCTCAAAC
AGACATTATTATGTCAAGGAAAAGCTGCAAAAAATTGTGGCGCGCGTGTCCGGCATTTTAAGCGAGCATGCGAAGGCGAG
CGGGTTTGCACCGGTTGGGCTTGAGCTTGGTTTTGGCGGGAAAGGCCCGCTGCCGCCTCTTACATTCACATTAAAAAACG
GCTGCACGATGGAGCTTGTCGGACGCATTGACCGCGTGGATAAAGCGGAAAGTTCAAAAGGGCTGCTGCTGAGAATAGTG
GATTATAAATCCAGCGACAAGGGTCTTGATTTGTCAGAGGTATACTACGGCCTTGCTCTGCAAATGCTCACGTACCTTGA
TTTATCCATTACGTATTCGGCAGATTGGCTCGGCATGAAGGCTACACCGGCCGGCGTTCTTTATTTTCATATTCACGACC
CGATGATTCAAGCCAGCGTTCCGCTCGGGCTTGATGAAATAGAGCAGGAAATTTTTAAGAAATTTAAGATGAAAGGCCTG
CTGCTCGGCGATCAGGAAGCGATTCGATTAATGGATACGACGCTCCAAGAGGGAAGGTCAAATATCATTAATGCCGGGCT
TAAAAAAGACGGTTCATTAAGGTCTGATTCAGCGGCGGTCGGAGAAGAGGAATTTCATCTGCTGACACAGCACGTGCGGC
GGACCTTTCAGGAGGCCGGCGAACAAATCACCGACGGGTGTGTGTCGATTGAGCCGTATAAGCTGAAAGATAAAACACCC
TGCACATACTGCTCATTCAAATCAGTATGCCAGTTCGATGAATCATTGGAAGAAAACGAATATCGCACGCTGAAAGCGGA
AAAAGATCATACCATATTAGATTGGCTGAAAAAGGAGGGAGATGGCGATGCAAATTCCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(791-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

88.765

100

0.888

Multiple sequence alignment