Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   CSB85_RS20625 Genome accession   NZ_CP027174
Coordinates   4282734..4283159 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain AR_0230     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4277734..4288159
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CSB85_RS20610 (CSB85_4166) pilX 4278298..4278885 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  CSB85_RS20615 (CSB85_4167) pilY1 4278897..4282388 (+) 3492 WP_003102607.1 type 4a pilus biogenesis protein PilY1 -
  CSB85_RS20620 (CSB85_4168) pilY2 4282390..4282737 (+) 348 WP_003161774.1 type 4a fimbrial biogenesis protein PilY2 -
  CSB85_RS20625 (CSB85_4169) comF 4282734..4283159 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  CSB85_RS20630 (CSB85_4170) ispH 4283206..4284150 (-) 945 WP_003134939.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  CSB85_RS20635 (CSB85_4171) fkpB 4284236..4284676 (-) 441 WP_003161775.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  CSB85_RS20640 (CSB85_4172) lspA 4284669..4285178 (-) 510 WP_003102615.1 signal peptidase II -
  CSB85_RS20645 (CSB85_4173) ileS 4285171..4288002 (-) 2832 WP_003102617.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=231024 CSB85_RS20625 WP_003094721.1 4282734..4283159(+) (comF) [Pseudomonas aeruginosa strain AR_0230]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=231024 CSB85_RS20625 WP_003094721.1 4282734..4283159(+) (comF) [Pseudomonas aeruginosa strain AR_0230]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGCTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAACTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACACCCACCAGCACTACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383

Multiple sequence alignment