Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   B6257_RS18680 Genome accession   NZ_CP021011
Coordinates   3699907..3700341 (+) Length   144 a.a.
NCBI ID   WP_007611576.1    Uniprot ID   A0A172XIP3
Organism   Bacillus velezensis strain GFP-2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3694907..3705341
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B6257_RS18675 (B6257_18575) - 3698407..3699618 (-) 1212 WP_071392577.1 cytochrome P450 family protein -
  B6257_RS18680 (B6257_18580) nucA/comI 3699907..3700341 (+) 435 WP_007611576.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  B6257_RS18685 (B6257_18585) - 3700441..3701157 (+) 717 WP_015417517.1 YoaK family protein -
  B6257_RS18690 (B6257_18590) - 3701191..3701553 (-) 363 WP_007410383.1 hypothetical protein -
  B6257_RS18695 (B6257_18595) - 3701746..3703074 (-) 1329 WP_071391804.1 S8 family peptidase -
  B6257_RS18700 (B6257_18600) - 3703253..3703486 (+) 234 WP_071391803.1 hypothetical protein -
  B6257_RS18705 (B6257_18605) - 3703742..3704449 (+) 708 WP_003154076.1 poly-gamma-glutamate hydrolase family protein -
  B6257_RS18710 (B6257_18610) - 3704511..3704963 (+) 453 WP_070081858.1 OsmC family protein -
  B6257_RS18715 (B6257_18615) - 3704977..3705330 (-) 354 WP_003154073.1 DMT family transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15528.47 Da        Isoelectric Point: 7.2418

>NTDB_id=227849 B6257_RS18680 WP_007611576.1 3699907..3700341(+) (nucA/comI) [Bacillus velezensis strain GFP-2]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=227849 B6257_RS18680 WP_007611576.1 3699907..3700341(+) (nucA/comI) [Bacillus velezensis strain GFP-2]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCAGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGCTATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A172XIP3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.452

86.111

0.486

Multiple sequence alignment