Detailed information NTG0022718

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PSPTO_RS04880
• Genome accession: NC_004578
• Coordinates: 1003193..1004065 (-)
• Length: 290 a.a.
• NCBI ID: WP_005770019.1
• Uniprot ID: Q888U2
• Organism: Pseudomonas syringae pv. tomato str. DC3000
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 998193..1009065

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSPTO_RS04850 (PSPTO0917)	-	998903..1000201 (-)	1299	WP_005614782.1	NAD(P)/FAD-dependent oxidoreductase	-
PSPTO_RS04855 (PSPTO0919)	-	1000418..1000951 (-)	534	WP_005770014.1	MOSC domain-containing protein	-
PSPTO_RS04860 (PSPTO0920)	-	1000948..1001577 (-)	630	WP_003381213.1	DUF1780 domain-containing protein	-
PSPTO_RS04865	-	1001774..1002100 (+)	327	WP_005770015.1	hypothetical protein	-
PSPTO_RS04870 (PSPTO0922)	yacG	1002367..1002576 (-)	210	WP_003381207.1	DNA gyrase inhibitor YacG	-
PSPTO_RS04875 (PSPTO0923)	coaE	1002573..1003196 (-)	624	WP_005770017.1	dephospho-CoA kinase	-
PSPTO_RS04880 (PSPTO0924)	pilD	1003193..1004065 (-)	873	WP_005770019.1	prepilin peptidase	Machinery gene
PSPTO_RS04885 (PSPTO0925)	pilC	1004067..1005284 (-)	1218	WP_011103349.1	type II secretion system F family protein	Machinery gene
PSPTO_RS04890 (PSPTO0926)	pilB	1005287..1006981 (-)	1695	WP_011103350.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PSPTO_RS04895 (PSPTO0927)	-	1007206..1007634 (+)	429	WP_011103351.1	pilin	-
PSPTO_RS04900 (PSPTO0928)	-	1007830..1008198 (-)	369	WP_011103352.1	winged helix-turn-helix transcriptional regulator	-
PSPTO_RS04905 (PSPTO0929)	gstA	1008356..1008976 (+)	621	WP_011103353.1	glutathione transferase GstA	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31982.22 Da
• Isoelectric Point: 8.1553

>NTDB_id=22718 PSPTO_RS04880 WP_005770019.1 1003193..1004065(-) (pilD) [Pseudomonas syringae pv. tomato str. DC3000]
MPLLDLLASSPLAFVTTCCLLGLIVGSFLNVVVYRLPKMMERDWKVQSREMLGLPAEPDQPVFNLILPHSSCPHCAHKIR
PWENLPVISYLLLRGKCSQCKAPISKRYPVVELTCAVLSAYVAWHFGFGWQAAAMLVLSWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIVNAFGLFTSLSDALWGAVAGYLTLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=22718 PSPTO_RS04880 WP_005770019.1 1003193..1004065(-) (pilD) [Pseudomonas syringae pv. tomato str. DC3000]
ATGCCCCTCCTCGACCTCTTGGCCAGCTCTCCCCTGGCCTTCGTCACCACCTGCTGCCTACTTGGCCTGATCGTCGGAAG
CTTCCTCAACGTGGTCGTCTACCGCCTCCCAAAAATGATGGAGCGCGACTGGAAAGTACAATCACGGGAAATGCTAGGCC
TCCCTGCCGAACCGGACCAACCCGTCTTCAACCTGATCCTGCCGCACTCAAGCTGCCCGCACTGCGCGCACAAGATCCGC
CCGTGGGAAAACCTGCCGGTCATCAGTTACCTGTTGCTGCGCGGCAAATGCTCGCAATGCAAAGCCCCCATCAGCAAGCG
CTACCCAGTGGTGGAGCTGACCTGCGCAGTGTTGTCGGCCTACGTCGCCTGGCATTTCGGCTTCGGCTGGCAGGCGGCTG
CGATGCTGGTGTTGAGCTGGGGTTTGCTGGCGATGAGTCTGATCGATGCCGATCATCAGCTGTTACCTGACTCTTTGGTG
CTGCCACTGCTGTGGCTGGGCCTGATCGTTAACGCCTTTGGGCTGTTCACCTCGCTGAGCGACGCGCTGTGGGGTGCAGT
GGCCGGTTATCTGACGCTGTGGTCGGTGTTCTGGCTTTTCAAGTTGGTGACGGGCAAGGAAGGCATGGGCTATGGCGACT
TCAAATTGCTGGCCATGCTTGGCGCGTGGGGTGGCTGGCAGATTCTGCCGTTGACCATTCTGTTGTCGTCGCTGGTGGGT
GCGGTACTTGGGGTGATCATGATGCGCGTGCGCAGGGTCGAAAGTGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTATATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

3D structure

• AlphaFold DB: Q888U2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.064	97.586	0.528
pilD	Vibrio campbellii strain DS40M4	51.943	97.586	0.507
pilD	Acinetobacter baumannii D1279779	52.555	94.483	0.497
pilD	Acinetobacter nosocomialis M2	51.825	94.483	0.49
pilD	Neisseria gonorrhoeae MS11	51.866	92.414	0.479