Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   B9L64_RS04645 Genome accession   NZ_CP020915
Coordinates   943917..944954 (-) Length   345 a.a.
NCBI ID   WP_029726718.1    Uniprot ID   -
Organism   Bacillus subtilis strain 50-1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 938917..949954
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B9L64_RS04595 tasA 939014..939799 (-) 786 WP_014664586.1 biofilm matrix protein TasA -
  B9L64_RS04600 sipW 939863..940435 (-) 573 WP_072692741.1 signal peptidase I SipW -
  B9L64_RS04605 tapA 940419..941180 (-) 762 WP_029726724.1 amyloid fiber anchoring/assembly protein TapA -
  B9L64_RS04610 yqzG 941452..941778 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  B9L64_RS04615 spoIITA 941820..941999 (-) 180 WP_029726723.1 YqzE family protein -
  B9L64_RS04620 comGG 942071..942445 (-) 375 WP_029726722.1 ComG operon protein ComGG Machinery gene
  B9L64_RS04625 comGF 942446..942829 (-) 384 WP_029726721.1 ComG operon protein ComGF Machinery gene
  B9L64_RS04630 comGE 942855..943202 (-) 348 WP_014480255.1 ComG operon protein 5 Machinery gene
  B9L64_RS04635 comGD 943186..943617 (-) 432 WP_029726720.1 comG operon protein ComGD Machinery gene
  B9L64_RS04640 comGC 943607..943903 (-) 297 WP_029726719.1 comG operon protein ComGC Machinery gene
  B9L64_RS04645 comGB 943917..944954 (-) 1038 WP_029726718.1 comG operon protein ComGB Machinery gene
  B9L64_RS04650 comGA 944941..946011 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  B9L64_RS04655 - 946224..946421 (-) 198 WP_029726717.1 hypothetical protein -
  B9L64_RS04660 corA 946423..947376 (-) 954 WP_004399136.1 magnesium transporter CorA -
  B9L64_RS04665 yqhB 947519..948847 (+) 1329 WP_029726716.1 hemolysin family protein -
  B9L64_RS04670 rsbRD 948900..949736 (-) 837 WP_003230149.1 RsbT co-antagonist protein RsbRD -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39702.10 Da        Isoelectric Point: 9.6257

>NTDB_id=227064 B9L64_RS04645 WP_029726718.1 943917..944954(-) (comGB) [Bacillus subtilis strain 50-1]
MKKIRKVWSLKDQARLLKRLGEMTAGGYTLLDGLRLMELQMNKRQAADLTDSVTCLREGAPFYQVLKSLSFHKEAVGICY
FAETHGELPASMIQSGELLERKIAQADQLKRVLRYPLFLIFTVAVMFYMLQSIIIPQFSGIYQSMNMETSRSTDILFAFF
QHIDLVIILLVLFTAGIGIYYWLVFKKKSPARQMLICIRIPLVGKLVKLFNSYFFSLQLSSLLKSGLSIYDSLNAFKHQT
FLPFYRCEAEQLIERLKAGESIESAICGSPFYETDLSKVISHGQLSGRLDRELFTYSQFILQRLEHKAQKWTGILQPMIY
GFVAAMILLVYLSMLVPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=227064 B9L64_RS04645 WP_029726718.1 943917..944954(-) (comGB) [Bacillus subtilis strain 50-1]
ATGAAAAAGATTAGAAAAGTCTGGTCGTTAAAGGATCAAGCGAGGTTATTAAAGAGGCTCGGTGAAATGACTGCGGGCGG
ATATACGCTTCTGGATGGATTACGCCTGATGGAACTTCAGATGAATAAGAGGCAGGCGGCTGACTTGACTGATTCGGTCA
CTTGTTTGAGGGAAGGGGCTCCGTTTTATCAAGTACTAAAGAGTTTGTCATTTCATAAGGAAGCCGTAGGTATTTGTTAT
TTTGCTGAAACACATGGTGAACTGCCTGCTTCCATGATCCAGAGCGGAGAGCTGCTGGAACGAAAAATTGCACAGGCAGA
CCAGCTGAAAAGAGTGCTGCGCTATCCGCTTTTCCTCATCTTTACGGTCGCTGTCATGTTTTATATGTTACAGTCCATCA
TCATTCCTCAGTTTTCCGGTATCTATCAATCGATGAATATGGAAACCTCACGTTCAACCGATATACTTTTTGCTTTTTTT
CAGCATATTGATCTTGTGATCATTTTGCTTGTTCTTTTTACAGCAGGTATCGGGATTTATTATTGGCTTGTGTTTAAGAA
AAAATCACCTGCCCGGCAAATGCTGATTTGTATCAGGATTCCTTTGGTTGGAAAGCTTGTCAAGCTGTTTAACAGCTACT
TTTTTTCTTTGCAGCTAAGCAGCCTTTTAAAATCAGGCCTCTCAATTTATGACAGCCTTAATGCATTTAAACATCAAACG
TTTCTCCCTTTCTACCGCTGCGAGGCTGAACAATTGATTGAACGGCTAAAAGCCGGTGAGTCAATTGAATCCGCTATTTG
TGGAAGCCCTTTTTATGAAACTGATTTATCAAAAGTCATATCTCACGGCCAGCTGAGCGGCCGATTGGATCGGGAGCTTT
TCACATACAGCCAATTCATATTACAGCGGCTGGAACACAAAGCGCAAAAATGGACAGGCATCCTTCAGCCAATGATTTAT
GGATTTGTTGCAGCGATGATCTTACTTGTGTATTTATCTATGCTTGTGCCTATGTATCAGATGATGAATCAAATGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(17-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

99.381

93.623

0.93

Multiple sequence alignment