Detailed information NTG0022597

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: SPYM18_RS07080
• Genome accession: NC_003485
• Coordinates: 1363718..1366102 (-)
• Length: 794 a.a.
• NCBI ID: WP_011018057.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes MGAS8232
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1358718..1371102

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPYM18_RS07060 (spyM18_1634)	pknB	1358736..1360634 (-)	1899	WP_011018054.1	Stk1 family PASTA domain-containing Ser/Thr kinase	Regulator
SPYM18_RS07065 (spyM18_1635)	-	1360631..1361371 (-)	741	WP_002983660.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
SPYM18_RS07070 (spyM18_1637)	rsmB	1361409..1362731 (-)	1323	WP_011018055.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
SPYM18_RS07075 (spyM18_1638)	fmt	1362721..1363656 (-)	936	WP_011018056.1	methionyl-tRNA formyltransferase	-
SPYM18_RS07080 (spyM18_1639)	priA	1363718..1366102 (-)	2385	WP_011018057.1	primosomal protein N'	Machinery gene
SPYM18_RS07085 (spyM18_1640)	rpoZ	1366167..1366484 (-)	318	WP_002983650.1	DNA-directed RNA polymerase subunit omega	-
SPYM18_RS07090 (spyM18_1642)	gmk	1366500..1367135 (-)	636	WP_002983649.1	guanylate kinase	-
SPYM18_RS07095 (spyM18_1643)	-	1367245..1368852 (-)	1608	WP_011018058.1	ribonuclease Y	-
SPYM18_RS07100 (spyM18_1644)	-	1368982..1369914 (-)	933	WP_050579051.1	LysR family transcriptional regulator	-

Sequence

Protein

• Length: 794 a.a.
• Molecular weight: 90285.21 Da
• Isoelectric Point: 9.4613

>NTDB_id=22597 SPYM18_RS07080 WP_011018057.1 1363718..1366102(-) (priA) [Streptococcus pyogenes MGAS8232]
MEKVAHVIVDIPLMQTDKPFSYGIPKELVSLVQLGSRVHVPFGKGNRLLQGFIIGFGQEDSSSLKLIQTVLDPEPVLNQE
QLTLADQLRKTVFSYKITLLKAMIPNLLNSNYDKVLRPESGLKKPDRDFLFEGQPSVLYSTLDREKGKIALKGIQAGHIT
VSYLAKDKKNLKTEKYYHVDLDALAVHPISSRAKKRQLLKDYLLTHTKEAKLATLYQAFSRDVVTYFVTNHLIRIDERPI
DRSESYFDQIKPSSFLTLNEQQASAVTEIVEQIGKPSKPFLIEGITGSGKTEVYLHIIEAVLKQDKTAIVLVPEISLTPQ
MTSRFISRFGKQVAIMHSGLSDGEKFDEWRKIKTGQAKVVVGARSAIFSPLERIGAIIIDEEHESTYKQESNPRYHAREV
ALLRAKHHQAVVVMGSATPSIESRARASKGVYHFIQLTQRANPLAKIPEVTIVDFRDYIGQQAVSNFTPYLIDKIKERLV
KKEQVVLMLNRRGYSSFVMCRDCGYVDKCPNCDISLTLHMDTKTMNCHYCGFQKPIPITCPECHSNSIRYYGTGTQKAFD
ELQGVIPEAKILRMDVDTTRKKRSHKTILDSFGRQEADILLGTQMIAKGLDFPNVTLVGVLNADTSLNLPDFRASEKTFQ
LLTQVAGRAGRAHKPGEVLIQTYNPDHYAIQLAKQQDFEAFYRYEMAIRRQMAYPPYYFTVGITLSHRLEASVVKKAYQV
TELLKSHLSNNIKILGPTPKPIARTHNLYHYQILLKYRFEDNLEETLNRILDWSQEADNRHLKLIIDCEPQQFL

Nucleotide

• Length: 2385 bp

>NTDB_id=22597 SPYM18_RS07080 WP_011018057.1 1363718..1366102(-) (priA) [Streptococcus pyogenes MGAS8232]
ATGGAAAAAGTAGCTCATGTGATTGTAGATATTCCATTGATGCAAACGGACAAGCCATTTTCTTATGGTATTCCAAAAGA
GTTAGTTTCTCTCGTGCAGCTTGGTTCTCGTGTGCATGTTCCTTTTGGAAAAGGGAATCGTTTGTTGCAAGGCTTTATTA
TTGGTTTTGGCCAAGAAGATTCAAGCTCTCTTAAACTGATTCAGACCGTTTTAGATCCTGAGCCAGTCCTAAACCAAGAG
CAATTGACCCTTGCTGATCAGCTTCGAAAAACAGTTTTTTCTTATAAAATCACGCTCTTAAAAGCAATGATTCCTAATCT
TCTTAACTCTAACTATGATAAAGTGCTAAGGCCAGAGTCAGGTCTAAAAAAGCCTGATAGAGACTTTCTTTTTGAAGGAC
AACCATCTGTCTTATATTCTACCTTAGACCGCGAGAAGGGAAAAATAGCTCTTAAGGGCATACAAGCTGGGCATATTACT
GTTAGTTATTTAGCCAAAGACAAGAAAAACCTAAAGACAGAAAAATATTATCACGTCGACTTAGACGCCTTAGCGGTACA
TCCCATCTCTAGTCGTGCCAAAAAGCGTCAATTATTAAAAGACTATCTGCTAACCCATACGAAGGAAGCTAAACTTGCAA
CGCTTTATCAAGCCTTTTCTAGAGATGTGGTGACTTACTTTGTGACAAATCACTTAATTCGTATTGACGAACGTCCAATT
GATCGCAGTGAGTCCTATTTTGACCAGATCAAACCATCTTCTTTTTTAACCTTAAATGAACAGCAGGCGTCAGCTGTTAC
TGAAATTGTTGAACAAATTGGGAAACCGAGTAAACCTTTTTTGATTGAAGGGATTACAGGTTCGGGTAAGACAGAAGTTT
ATCTTCATATCATCGAAGCGGTTTTAAAACAGGATAAGACAGCCATTGTTTTAGTGCCAGAAATTTCGTTGACTCCTCAG
ATGACCTCTCGTTTTATTTCCCGTTTCGGAAAGCAAGTGGCTATTATGCATTCAGGCTTATCAGATGGTGAAAAGTTTGA
TGAGTGGCGCAAGATAAAAACAGGACAGGCTAAGGTTGTGGTTGGTGCCCGCTCTGCTATTTTCTCCCCACTAGAGCGGA
TTGGTGCAATTATTATCGATGAGGAGCATGAATCAACCTATAAACAAGAAAGTAATCCACGATACCATGCCAGAGAGGTT
GCCCTGCTCAGAGCTAAACACCATCAAGCAGTTGTGGTTATGGGCTCTGCTACGCCAAGTATTGAAAGCCGAGCAAGGGC
TAGTAAGGGTGTCTATCATTTTATTCAATTAACGCAAAGAGCAAACCCTCTCGCTAAGATTCCAGAAGTAACGATTGTTG
ATTTTCGTGATTATATTGGACAGCAAGCAGTTAGTAATTTTACCCCATATTTGATTGATAAGATTAAAGAACGTTTGGTT
AAAAAAGAACAAGTTGTCTTAATGTTAAATAGACGTGGTTATTCTAGTTTTGTGATGTGTCGGGACTGTGGTTATGTGGA
CAAATGTCCCAACTGTGATATCTCTTTAACCCTTCACATGGATACTAAAACTATGAATTGCCATTACTGTGGGTTTCAAA
AACCTATCCCAATTACTTGTCCTGAGTGTCATAGCAATAGTATCCGCTATTATGGAACAGGGACGCAAAAAGCTTTTGAT
GAATTGCAAGGGGTTATTCCAGAAGCTAAGATCTTGAGAATGGATGTTGACACCACACGAAAAAAAAGAAGTCACAAAAC
CATACTAGATAGTTTTGGTCGCCAGGAAGCAGATATTTTGTTAGGGACGCAAATGATTGCCAAAGGACTGGATTTTCCAA
ATGTGACTTTAGTTGGGGTACTAAATGCAGATACCTCCTTAAATTTGCCAGATTTTCGAGCGTCAGAAAAAACGTTTCAA
TTGTTAACACAGGTTGCTGGTCGGGCAGGCCGTGCCCATAAGCCAGGGGAAGTCTTGATTCAAACTTATAATCCCGATCA
CTATGCAATTCAGTTGGCTAAACAACAAGATTTTGAAGCCTTTTACCGTTATGAAATGGCGATTCGCCGTCAGATGGCTT
ATCCCCCTTACTACTTTACAGTGGGTATTACGCTTTCGCATCGTTTAGAAGCATCGGTTGTAAAAAAAGCTTATCAAGTT
ACAGAACTGCTAAAGAGTCACTTATCGAATAATATTAAAATATTAGGACCGACTCCTAAGCCAATTGCTAGAACACACAA
TTTATACCACTATCAAATTTTGTTAAAATACCGTTTTGAAGATAACCTAGAAGAGACGCTCAATCGTATTCTTGATTGGA
GTCAAGAAGCAGATAATCGTCATTTAAAACTAATTATTGATTGTGAACCGCAACAATTTTTATAA

Domains

Predicted by InterProScan.

• 3DNA-binding domain (3BD) (PriA_3primeBD) (7-105)

• Type III restriction enzyme, res subunit (ResIII) (257-420)

• Helicase conserved C-terminal domain (Helicase_C) (568-652)

• Primosomal protein N C-terminal domain (PriA_C) (696-790)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (509-535)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	46.832	100	0.475