Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   B5D77_RS20230 Genome accession   NZ_CP020664
Coordinates   3971326..3972441 (-) Length   371 a.a.
NCBI ID   WP_008196678.1    Uniprot ID   -
Organism   Microcystis sp. MC19     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3966326..3977441
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B5D77_RS20205 (B5D77_19960) - 3966801..3967028 (+) 228 WP_008196685.1 type II toxin-antitoxin system HicB family antitoxin -
  B5D77_RS20215 (B5D77_19965) - 3967540..3969666 (-) 2127 WP_106909525.1 secretin N-terminal domain-containing protein -
  B5D77_RS20220 (B5D77_19970) - 3969740..3970549 (-) 810 WP_008196681.1 hypothetical protein -
  B5D77_RS20225 (B5D77_19975) - 3970546..3971316 (-) 771 WP_008196679.1 PilN domain-containing protein -
  B5D77_RS20230 (B5D77_19980) pilM 3971326..3972441 (-) 1116 WP_008196678.1 type IV pilus assembly protein PilM Machinery gene
  B5D77_RS20240 (B5D77_19990) ilvC 3973379..3974371 (+) 993 WP_008196674.1 ketol-acid reductoisomerase -
  B5D77_RS20245 (B5D77_19995) - 3974475..3975263 (-) 789 WP_008196672.1 hypothetical protein -
  B5D77_RS20250 (B5D77_20000) - 3975363..3976148 (-) 786 WP_008196670.1 bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG -
  B5D77_RS20255 (B5D77_20005) - 3976271..3976810 (-) 540 WP_008196668.1 pentapeptide repeat-containing protein -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41118.01 Da        Isoelectric Point: 4.3318

>NTDB_id=225348 B5D77_RS20230 WP_008196678.1 3971326..3972441(-) (pilM) [Microcystis sp. MC19]
MVSFLKKLLTKKNQGVGLEITPERINVAQIAKQGQNYQLVKYCWSDIPEGIFEAGKIVDSPALAELIQEVLKENKINSKR
VATGVPMRESIIRIIPIPSELDDQELRDLVLNHEASLYLPYPREEVDLDYQKLGYFQDEDGIEKVQVLLVATRREITDAY
METFQQAGLQVDVLEINSFALIRTIREHLRQFSSNEAAVIVDIEFDNTEIAIVVDGVPQFSRTVPIGTFQLQNALSRAMN
LPTSRSPEVLLGMTIPITAFDSLSSNTGTSGAATNAGMTALMRVLGELTDELRRSINFYLNQSDELEIVQLLLAGPGGGL
AQLDEYFTQRLSVPTMAIDPIASLNLDTTQEIPNTERPGLGTVLGLGLREL

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=225348 B5D77_RS20230 WP_008196678.1 3971326..3972441(-) (pilM) [Microcystis sp. MC19]
ATGGTAAGTTTTTTAAAGAAGCTATTGACAAAAAAAAATCAAGGTGTTGGGTTAGAGATTACCCCAGAACGGATTAACGT
CGCTCAGATCGCCAAACAGGGACAAAATTATCAATTAGTTAAATATTGCTGGTCGGATATCCCGGAAGGCATCTTTGAGG
CGGGAAAAATCGTCGATTCTCCCGCTTTAGCGGAATTAATCCAAGAAGTCCTCAAGGAAAATAAAATTAACAGCAAACGA
GTCGCCACTGGTGTGCCGATGCGCGAGTCAATTATCCGGATTATCCCGATTCCCTCGGAATTGGACGATCAGGAATTGCG
CGATCTGGTCTTAAATCATGAGGCTAGTTTATACCTTCCCTATCCTCGCGAGGAAGTGGATCTCGACTACCAAAAACTGG
GCTACTTTCAGGATGAAGACGGGATCGAAAAAGTGCAGGTTTTGCTGGTAGCCACTCGTCGGGAGATTACCGATGCCTAT
ATGGAAACCTTCCAACAGGCGGGATTACAGGTAGATGTGCTAGAAATTAATAGTTTTGCCCTAATTCGGACGATTCGCGA
ACATTTGCGACAGTTTAGTTCCAATGAAGCTGCCGTTATTGTTGATATAGAATTTGATAACACGGAAATTGCCATCGTGG
TGGACGGCGTTCCCCAATTTTCCCGCACTGTCCCCATTGGCACTTTCCAGCTACAAAATGCCCTCTCTAGGGCGATGAAT
TTGCCCACCTCCCGCAGTCCTGAGGTGCTTTTGGGTATGACAATCCCGATTACTGCCTTTGATAGTCTGAGCAGCAATAC
CGGCACTTCCGGAGCGGCTACTAACGCCGGGATGACGGCACTGATGCGGGTTTTAGGGGAATTAACCGATGAATTGCGGC
GATCGATTAATTTCTATCTTAACCAAAGTGATGAACTGGAAATCGTCCAGTTACTTTTAGCCGGTCCCGGGGGCGGTTTA
GCACAACTAGACGAATATTTTACTCAACGTCTCAGCGTCCCCACCATGGCCATCGATCCGATCGCATCACTGAACTTAGA
CACAACTCAAGAGATTCCTAATACCGAAAGACCAGGATTAGGAACAGTTCTCGGTTTAGGATTACGGGAGTTATAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-241)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

75.405

99.73

0.752

Multiple sequence alignment