Detailed information NTG0223027

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: C2I05_RS19600
• Genome accession: NZ_CP026039
• Coordinates: 3703586..3706003 (+)
• Length: 805 a.a.
• NCBI ID: WP_120028083.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain PK1_2
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 3698586..3711003

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C2I05_RS19575 (C2I05_19550)	yloC	3700238..3701113 (+)	876	WP_009967215.1	YicC/YloC family endoribonuclease	-
C2I05_RS19580 (C2I05_19555)	remA	3701190..3701459 (+)	270	WP_003154355.1	extracellular matrix/biofilm regulator RemA	-
C2I05_RS19585 (C2I05_19560)	gmk	3701467..3702081 (+)	615	WP_003232083.1	guanylate kinase	-
C2I05_RS19590 (C2I05_19565)	rpoZ	3702085..3702288 (+)	204	WP_003221520.1	DNA-directed RNA polymerase subunit omega	-
C2I05_RS19595 (C2I05_19570)	coaBC	3702369..3703589 (+)	1221	WP_015252114.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
C2I05_RS19600 (C2I05_19575)	priA	3703586..3706003 (+)	2418	WP_120028083.1	primosomal protein N'	Machinery gene
C2I05_RS19605 (C2I05_19580)	def	3706030..3706512 (+)	483	WP_015383630.1	peptide deformylase	-
C2I05_RS19610 (C2I05_19585)	fmt	3706517..3707470 (+)	954	WP_120028084.1	methionyl-tRNA formyltransferase	-
C2I05_RS19615 (C2I05_19590)	rsmB	3707457..3708800 (+)	1344	WP_120028085.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
C2I05_RS19620 (C2I05_19595)	rlmN	3708804..3709895 (+)	1092	WP_100506558.1	23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN	-
C2I05_RS19625 (C2I05_19600)	prpC	3709902..3710666 (+)	765	WP_041850843.1	protein-serine/threonine phosphatase PrpC	-

Sequence

Protein

• Length: 805 a.a.
• Molecular weight: 91335.37 Da
• Isoelectric Point: 8.5313

>NTDB_id=223027 C2I05_RS19600 WP_120028083.1 3703586..3706003(+) (priA) [Bacillus subtilis strain PK1_2]
MNFAEVIVDVSTKNIDRPFDYKIPDHLKGMIKTGMRVIVPFGPRKIQGFVTAVKEASDLSGKSVKEVEDLLDLTPVLTEE
LMDLSSWLSNKTLSFKITALQAMLPAALKAKYEKELKIAHGADLPPQVERLFSETKTLLYSDIPDHDTLKLIQRHVQKGA
IDVTYKVAQKTNKKMVRHIQANASKEELAKQTEGLSRQAAKQQAILHFLISEPEGVKIPAAELCKKTDTSSATIKTLIQK
GLLKESYEEVYRDPYHDKMFKKTEPLPLTDEQRAAFEPIRETLDSDEHKVFLLHGVTGSGKTEIYLQSIEKVLAKGKEAI
VLVPEISLTPQMVNRFKGRFGSQVAVMHSGLSTGEKYDEWRKIHRKEVRLVVGARSAIFAPFENLGMIIIDEEHESSYKQ
EEMPRYHAKEVAIKRAEHHSCPVVLGSATPTLESYARAQKGVYELLSLKHRVNHRVMPEVSLVDMREELRNGNRSMFSVE
LMEKLEETIAKGEQAVLFLNKRGYSSFVMCRDCGYVPQCPHCDISMTYHRYGQRLKCHYCGHEEPVPHTCPECASEHIRF
FGTGTQRVEEELTKVLPSARVIRMDVDTTSRKGAHEKLLSAFGEGKADILLGTQMIAKGLDFPNVTLVGVLSADTTLHIP
DFRSAEKTFQLLTQVSGRAGRHEKPGHVIIQTYTPSHYSIQLTKTHDYETFYQHEMAHRREQSYPPYYYLALVTVSHEEV
AKAAVTAEKIAHFLKANCGADTKILGPSASPIARIKDRYRYQCVIKYKQETQLSALLKKILEHYKREIEQKHVMISIDMN
PYMMM

Nucleotide

• Length: 2418 bp

>NTDB_id=223027 C2I05_RS19600 WP_120028083.1 3703586..3706003(+) (priA) [Bacillus subtilis strain PK1_2]
ATGAATTTTGCAGAAGTCATCGTTGATGTCAGCACCAAAAATATAGACAGGCCTTTTGATTATAAAATCCCAGACCATCT
CAAGGGCATGATCAAAACGGGGATGCGGGTCATTGTTCCGTTTGGCCCCCGTAAGATTCAAGGGTTTGTGACAGCAGTCA
AAGAAGCATCTGACCTATCGGGAAAATCTGTCAAGGAAGTAGAGGATTTATTAGATCTTACACCTGTTCTGACAGAGGAG
CTTATGGATCTGTCATCATGGCTGTCAAACAAAACGCTGTCTTTCAAAATAACGGCGCTTCAAGCAATGCTGCCTGCCGC
CTTAAAGGCGAAATATGAAAAAGAATTAAAAATTGCACACGGCGCCGATCTTCCGCCGCAAGTGGAACGGCTGTTTTCTG
AAACAAAGACGCTGCTGTATTCAGATATACCCGATCATGATACGCTGAAACTGATTCAAAGGCATGTTCAAAAAGGGGCC
ATTGACGTGACGTATAAAGTCGCGCAAAAAACCAATAAAAAAATGGTCAGGCATATTCAGGCAAATGCGAGTAAAGAAGA
GCTTGCTAAGCAGACTGAGGGATTGTCCCGACAAGCAGCAAAACAGCAGGCGATTCTTCACTTTCTCATCTCTGAGCCGG
AAGGTGTGAAAATTCCTGCAGCGGAATTGTGCAAAAAAACGGACACGAGCTCTGCAACCATCAAAACCCTTATCCAAAAA
GGGCTTTTGAAGGAAAGCTATGAAGAGGTTTACAGAGATCCCTATCATGACAAAATGTTCAAAAAAACAGAGCCCCTGCC
GCTGACAGACGAACAGAGGGCTGCCTTTGAGCCCATACGCGAGACATTGGACAGCGATGAGCATAAAGTGTTCCTCCTTC
ACGGCGTGACAGGAAGCGGAAAAACCGAAATTTATCTGCAATCAATTGAAAAAGTACTGGCGAAAGGAAAAGAAGCCATC
GTCCTCGTACCGGAAATATCGCTGACGCCGCAAATGGTCAACCGCTTCAAGGGCCGCTTCGGCTCTCAGGTGGCAGTCAT
GCACAGCGGTTTATCCACAGGAGAAAAATATGACGAGTGGCGTAAAATCCACCGGAAGGAAGTACGGCTTGTCGTCGGTG
CCAGATCCGCCATTTTTGCTCCGTTTGAAAATCTCGGAATGATAATCATCGATGAAGAGCACGAGTCTTCCTATAAACAG
GAAGAAATGCCGCGCTATCATGCGAAAGAGGTGGCAATCAAGCGGGCTGAGCATCACAGCTGCCCGGTTGTATTAGGGAG
TGCTACGCCGACATTAGAGTCATATGCTAGAGCGCAAAAGGGCGTATACGAGCTTCTTTCACTGAAGCACCGTGTTAACC
ACCGGGTGATGCCTGAGGTTTCGCTTGTCGATATGAGAGAGGAGCTTAGAAACGGCAATCGCTCGATGTTTTCAGTTGAA
TTGATGGAAAAGCTTGAAGAAACGATTGCCAAGGGTGAGCAGGCGGTGCTGTTTTTAAATAAGAGGGGCTATTCTTCCTT
TGTGATGTGCAGGGATTGCGGCTATGTCCCGCAATGCCCGCACTGCGACATTTCCATGACGTATCATCGTTACGGCCAAA
GGCTGAAATGCCATTATTGCGGACATGAAGAGCCTGTTCCGCACACTTGTCCTGAATGCGCAAGCGAGCATATCCGCTTT
TTCGGAACGGGAACACAGCGAGTGGAGGAAGAGCTGACAAAAGTGCTGCCAAGTGCGAGAGTGATTCGGATGGATGTTGA
CACGACATCACGGAAAGGCGCCCATGAAAAATTACTGTCAGCTTTCGGAGAAGGAAAAGCGGATATTCTTCTCGGAACGC
AAATGATCGCGAAAGGGCTTGATTTTCCGAATGTCACGCTTGTCGGAGTGTTAAGTGCTGATACAACACTTCATATTCCT
GATTTCAGATCGGCTGAAAAAACATTTCAGCTGTTAACGCAAGTCAGCGGCCGGGCAGGCAGGCATGAAAAGCCCGGCCA
TGTCATCATTCAAACGTATACACCGTCTCATTACAGTATCCAGCTGACGAAGACACACGATTATGAAACGTTCTATCAGC
ATGAAATGGCCCACAGGAGAGAGCAATCCTATCCGCCATACTACTATTTGGCTCTTGTGACTGTTTCTCATGAGGAAGTG
GCGAAAGCAGCGGTTACAGCTGAAAAGATCGCTCATTTTTTGAAAGCAAACTGCGGAGCTGACACGAAAATTCTCGGACC
TAGTGCGTCGCCGATAGCCAGGATCAAAGATAGATATCGCTATCAATGCGTGATAAAATACAAACAGGAAACCCAGTTAT
CCGCTTTATTGAAGAAGATACTGGAACATTATAAACGCGAAATTGAACAAAAACATGTAATGATTTCAATTGATATGAAT
CCTTATATGATGATGTAA

Domains

Predicted by InterProScan.

• Type III restriction enzyme, res subunit (ResIII) (267-431)

• Helicase conserved C-terminal domain (Helicase_C) (565-662)

• Primosomal protein N C-terminal domain (PriA_C) (706-801)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (519-545)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-105)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	99.255	100	0.993