Detailed information NTG0022072

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: XAC_RS16435
• Genome accession: NC_003919
• Coordinates: 3817245..3818981 (-)
• Length: 578 a.a.
• NCBI ID: WP_011052125.1
• Uniprot ID: A0AAI7ZH88
• Organism: Xanthomonas citri pv. citri str. 306
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	3795904..3816403	3817245..3818981	flank	842

Gene organization within MGE regions

Location: 3795904..3818981

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
XAC_RS24040 (XAC3223)	-	3795904..3797071 (+)	1168	Protein_3218	IS3 family transposase	-
XAC_RS16350 (XAC3224)	avrXacE2	3797831..3798901 (-)	1071	WP_011052114.1	type III secretion system effector avirulence protein AvrXacE2	-
XAC_RS16355 (XAC3225)	-	3798986..3800263 (-)	1278	WP_011052115.1	lytic murein transglycosylase	-
XAC_RS16360 (XAC3226)	-	3800396..3803386 (-)	2991	WP_040107658.1	Tn3-like element TnXax1 family transposase	-
XAC_RS16365 (XAC3227)	-	3803395..3804669 (-)	1275	Protein_3222	site-specific integrase	-
XAC_RS16375 (XAC3229)	-	3804862..3805938 (+)	1077	WP_011052118.1	DNA-binding protein	-
XAC_RS16380 (XAC3230)	xopAI	3806072..3806962 (+)	891	WP_011052119.1	type III secretion system effector XopAI	-
XAC_RS16385 (XAC3231)	-	3807382..3808188 (-)	807	WP_015463520.1	hypothetical protein	-
XAC_RS16390	-	3808293..3808577 (+)	285	WP_016849322.1	hypothetical protein	-
XAC_RS16395 (XAC3233)	-	3808805..3809920 (+)	1116	WP_011052122.1	IS1595 family transposase	-
XAC_RS16400 (XAC3234)	-	3809875..3810390 (-)	516	WP_011052123.1	hypothetical protein	-
XAC_RS16405 (XAC3235)	sucD	3810477..3811352 (-)	876	WP_005921370.1	succinate--CoA ligase subunit alpha	-
XAC_RS16410 (XAC3236)	sucC	3811377..3812546 (-)	1170	WP_005915812.1	ADP-forming succinate--CoA ligase subunit beta	-
XAC_RS16415 (XAC3237)	-	3812778..3814391 (+)	1614	WP_003488599.1	sensor histidine kinase	-
XAC_RS16420 (XAC3238)	pilR	3814716..3816110 (+)	1395	WP_015463522.1	sigma-54-dependent transcriptional regulator	Regulator
XAC_RS16425	-	3816333..3816500 (-)	168	WP_015463523.1	hypothetical protein	-
XAC_RS16430	pilB	3816632..3816751 (-)	120	WP_005915819.1	hypothetical protein	Machinery gene
XAC_RS16435 (XAC3239)	pilB	3817245..3818981 (-)	1737	WP_011052125.1	type IV-A pilus assembly ATPase PilB	Machinery gene

Sequence

Protein

• Length: 578 a.a.
• Molecular weight: 62517.63 Da
• Isoelectric Point: 5.4526

>NTDB_id=22072 XAC_RS16435 WP_011052125.1 3817245..3818981(-) (pilB) [Xanthomonas citri pv. citri str. 306]
MNSVVTANLVGITGIARRLVQDGAVEEAVARSAMDQASAAKVPLPQWFAEKKLVTASQLAAANAVEFGMPLLDVSAFDAS
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSALG
DDEEGMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALM
KAANGVTSLAEINRVTKD

Nucleotide

• Length: 1737 bp

>NTDB_id=22072 XAC_RS16435 WP_011052125.1 3817245..3818981(-) (pilB) [Xanthomonas citri pv. citri str. 306]
ATGAATAGCGTCGTGACCGCAAATTTGGTTGGAATAACGGGCATCGCGCGTCGTCTGGTGCAAGACGGTGCGGTCGAAGA
AGCAGTTGCGCGATCTGCGATGGATCAGGCATCTGCGGCAAAGGTTCCTCTGCCGCAATGGTTTGCTGAGAAGAAGTTGG
TCACTGCTTCCCAGCTCGCCGCTGCGAATGCGGTCGAGTTCGGCATGCCACTGTTGGACGTGTCGGCGTTCGACGCCAGC
CAAAACGCGGTCAAGCTGGTAAGTGAGGAGCTACTTCAGAAGCACCAAGTGCTACCGCTGTTCAAGCGCGGCAACCGGCT
GTTCGTAGGGGTGAGCAATCCGACCCAGACCCGGGCGCTGGATGACATCAAGTTTCATACGAACTTGGTAGTCGAGCCCA
TCCTTGTGGATGAAGATCAGATCCGTCGCACCCTGGAGCAATGGCAGGCCAGCAATGCTGCGCTGGGCTCCGCGCTCGGT
GACGACGAGGAGGGCATGGGGGATCTGGACGTCTCGGCCGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTTGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCAGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGATGGCTTGTTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCAGCGCGGTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTCAGCACCTTGCCGACCCTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTACGAGCCGGACCAACAGAAG
CTGTTCCTGGAAGCGATCCACAAGCCCTATGGCATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTT
GTACACCGCGCTGGGAATTCTCAACGACGAGACGCGCAATATCTCCACCGCGGAGGATCCGGTCGAAATCCGCTTGCCTG
GCGTCAATCAGGTGCAGCAGAACAACAAGCGTGGCATGACCTTCGCCGCGGCTTTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGTGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACGGGTCACATGGT
GCTGTCGACGTTGCACACCAACGATGCGCCGCAGACCATCGCACGTCTGATGAACATGGGCATCGCGCCCTACAACATCA
CCTCGTCGGTGACGCTGGTGATCGCGCAGCGGCTGGCGCGGCGCTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCT
GACAACGCATTGCTGGCCGAAGGATTCACGCCTGCCCAGCTTGCCGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
TGAGTGCACCGAAGGCTACAAGGGGCGTACCGGTATCTACCAGGTAATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAAGGCGGCAATGCGATGCAGATCGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCGCTGATG
AAGGCTGCGAATGGGGTGACCAGCCTGGCCGAGATCAATCGTGTGACGAAGGACTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (49-153)

• Type II/IV secretion system protein (T2SSE) (201-468)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	56.514	98.27	0.555
pilB	Acinetobacter baylyi ADP1	55.81	98.27	0.548
pilB	Legionella pneumophila strain ERS1305867	52.021	98.443	0.512
pilB	Vibrio cholerae strain A1552	49.306	99.654	0.491
pilF	Neisseria gonorrhoeae MS11	49.12	98.27	0.483
pilB	Vibrio parahaemolyticus RIMD 2210633	46.964	96.886	0.455
pilB	Vibrio campbellii strain DS40M4	45.518	98.443	0.448