Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   CWI20_RS28215 Genome accession   NZ_CP025056
Coordinates   6075033..6075629 (-) Length   198 a.a.
NCBI ID   WP_003095839.1    Uniprot ID   G3XD30
Organism   Pseudomonas aeruginosa strain PB367     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6070033..6080629
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWI20_RS28190 (CWI20_28800) aroB 6070188..6071294 (-) 1107 WP_003095831.1 3-dehydroquinate synthase -
  CWI20_RS28195 (CWI20_28805) aroK 6071344..6071862 (-) 519 WP_003095833.1 shikimate kinase AroK -
  CWI20_RS28200 (CWI20_28810) pilQ 6071874..6074018 (-) 2145 WP_003095835.1 type 4a pilus secretin PilQ Machinery gene
  CWI20_RS28205 (CWI20_28815) pilP 6074072..6074458 (-) 387 Protein_5587 type 4a pilus biogenesis lipoprotein PilP -
  CWI20_RS28210 (CWI20_28820) pilO 6074389..6075036 (-) 648 WP_237047402.1 type 4a pilus biogenesis protein PilO Machinery gene
  CWI20_RS28215 (CWI20_28825) pilN 6075033..6075629 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  CWI20_RS28220 (CWI20_28830) pilM 6075629..6076693 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  CWI20_RS28225 (CWI20_28835) - 6076878..6079349 (+) 2472 WP_003095841.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22213.55 Da        Isoelectric Point: 10.0895

>NTDB_id=217226 CWI20_RS28215 WP_003095839.1 6075033..6075629(-) (pilN) [Pseudomonas aeruginosa strain PB367]
MARINLLPWREELREQRKQQFLVILGGVLVASAALVFLGDQYFTAAIENQNARNDFLRKEIVVLDARIKEISELKSRRQQ
LLERMKIIQDLQGNRPIIGRVFDQLVRTLPDGVYFTDLKMTGKNIAIAGAAESNNRVSNLMRNMDASEWLTAPTLNEVKA
VTQGAVDQANVFQLTVQQTQPGEEDAKAKHGVAQGAKK

Nucleotide


Download         Length: 597 bp        

>NTDB_id=217226 CWI20_RS28215 WP_003095839.1 6075033..6075629(-) (pilN) [Pseudomonas aeruginosa strain PB367]
ATGGCACGGATCAACCTTCTACCCTGGCGCGAAGAGCTGCGCGAGCAGCGCAAGCAGCAGTTCCTGGTGATTCTTGGCGG
CGTCCTGGTGGCATCGGCGGCGCTGGTATTCCTTGGCGACCAGTACTTCACCGCGGCCATCGAGAACCAGAACGCCCGCA
ACGATTTCCTGCGCAAGGAAATCGTCGTACTCGACGCCCGGATCAAGGAAATCAGCGAACTGAAGTCGCGTCGCCAGCAA
TTGCTCGAGCGGATGAAGATCATCCAGGACCTGCAGGGCAACCGTCCCATCATCGGACGGGTGTTCGACCAGCTCGTGCG
GACCTTGCCCGACGGCGTGTATTTCACCGACCTGAAGATGACCGGCAAGAACATTGCCATCGCCGGCGCGGCCGAGTCCA
ACAACCGCGTTTCCAATCTCATGCGCAACATGGACGCGTCCGAGTGGTTGACCGCCCCGACCCTGAACGAGGTCAAGGCG
GTGACCCAGGGCGCGGTGGACCAGGCCAACGTGTTCCAACTGACCGTGCAGCAGACGCAGCCCGGCGAGGAAGACGCCAA
GGCGAAGCATGGGGTTGCGCAAGGAGCCAAGAAATGA

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(100-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 5EOU

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

41.714

88.384

0.369